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Bioimaging of Nitric Oxide with Fluorescent Indicators Based on the Rhodamine Chromophore
Abstract
Diaminofluoresceins are widely used for detection and imaging of nitric oxide (NO), but for biological applications, they have the disadvantages that the fluorescence of the fluorescein chromophore is pH-sensitive and overlaps the autofluorescence of cells. We have developed a membrane-permeable fluorescent indicator for NO based on the rhodamine chromophore, DAR-4M AM, which can be excited with 550-nm light. The fluorescence quantum yield of the product after reaction with NO is 840 times higher than that of DAR-4M. The detection limit of NO was 7 nM, and the fluorescence showed no pH dependency above pH 4. DAR-4M AM was successfully applied to practical bioimaging of NO produced in bovine aortic endothelial cells.
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Cited by
Methods to detect nitric oxide and its metabolites in biological samples
Bryan, N. S., & Grisham, M. B. (2007) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2007.04.026 · Cited by 1,005
Fluorescent and luminescent probes for detection of reactive oxygen and nitrogen species
Chen, X., Tian, X., Shin, I., & Yoon, J. (2011) · Chemical Society Reviews · doi:10.1039/c1cs15037e · Cited by 980
Tsikas, D. (2007) · Journal of Chromatography B · doi:10.1016/j.jchromb.2006.07.054 · Cited by 893
Tarpey, M. M., Wink, D. A., & Grisham, M. B. (2004) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00361.2003 · Cited by 673
Nagano, T., & Yoshimura, T. (2002) · Chemical Reviews · doi:10.1021/cr010152s · Cited by 414
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Metadata
- Authors
- Kojima, H., Hirotani, M., Nakatsubo, N., Kikuchi, K., Urano, Y., Higuchi, T., Hirata, Y., & Nagano, T.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-03-24
- Volume
- 73
- Issue
- 9
- Pages
- 1967-1973
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1980039414
- Cited by
- 292
- References
- 0
- FWCI
- 4.52
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kojima, H., Hirotani, M., Nakatsubo, N., Kikuchi, K., Urano, Y., Higuchi, T., Hirata, Y., & Nagano, T. (2001). Bioimaging of Nitric Oxide with Fluorescent Indicators Based on the Rhodamine Chromophore. Analytical Chemistry. https://doi.org/10.1021/ac001136i
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