Cited by 673Redox biology and oxidative stressAntioxidant Activity and Oxidative StressNitric Oxide and Endothelin Effects

Methods for detection of reactive metabolites of oxygen and nitrogen: In vitro and in vivo considerations

Tarpey, Margaret M., Wink, David A., Grisham, Matthew B.·2004·American Journal of Physiology-Regulatory, Integrative and Comparative Physiology

Abstract

Facile detection of reactive oxygen and nitrogen species in biologic systems is often problematic. This is a result of the numerous cellular mechanisms, both enzymatic and nonenzymatic involved in their catabolism/decomposition, the complex and overlapping nature of their reactivities, as well as the often limited intracellular access of detector systems. This review describes approaches to the direct and indirect measurement of different reactive metabolites of oxygen and nitrogen. Particular attention to a method's applicability for in vivo determinations will be addressed.

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Crossref · 10.1152/ajpregu.00361.2003

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153 of 153 references verified0 citation errors · 0 not checkable

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Authors
Tarpey, M. M., Wink, D. A., & Grisham, M. B.
Affiliations
University of Alabama at Birmingham (US)
Venue
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
Published
2004-03
Volume
286
Issue
3
Pages
R431-R444
ISSN
  • 0363-6119 (ISSN-L)
  • 1522-1490
OpenAlex
W2148883753
Cited by
673
References
153
Citations by year
2026: 7 · 2025: 5 · 2024: 33 · 2023: 19 · 2022: 16 · 2021: 24 · 2020: 27 · 2019: 23 · 2018: 23 · 2017: 22 · 2016: 45 · 2015: 25 · 2014: 40 · 2013: 42 · 2012: 49
FWCI
2.78
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01hl066110-04)
  • National Heart, Lung, and Blood Institute (HL-66110)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK64023, R01DK064023, P01DK043785, DK-43785)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Keywords
reactive oxygen species, reactive nitrogen species, reactive metabolites, detection methods, in vivo measurement, in vitro measurement, antioxidant enzymes
MeSH terms
Algorithms, Animals, Humans, Hydrogen Peroxide, Nitric Oxide, Superoxides, Tyrosine, Oxidants, Reactive Oxygen Species, Oxidative Stress, Reactive Nitrogen Species, S-Nitrosothiols, Peroxynitrous Acid

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