Methods for detection of reactive metabolites of oxygen and nitrogen: In vitro and in vivo considerations
Abstract
Facile detection of reactive oxygen and nitrogen species in biologic systems is often problematic. This is a result of the numerous cellular mechanisms, both enzymatic and nonenzymatic involved in their catabolism/decomposition, the complex and overlapping nature of their reactivities, as well as the often limited intracellular access of detector systems. This review describes approaches to the direct and indirect measurement of different reactive metabolites of oxygen and nitrogen. Particular attention to a method's applicability for in vivo determinations will be addressed.
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Some chemiluminescent probes show improved sensitivity but still have problems with interference.
“Some of the chemiluminescent probes show improved sensitivity [156] but there are still problems with interference [157].”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [156]
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Ingress and reactive chemistry of nitroxyl-derived species within human cells
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Self-citationS-Nitrosothiol Formation in Blood of Lipopolysaccharide-Treated Rats
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Self-citationOverstimation of verestimation of NADH-driven vascular oxidase activity due to lucigenin artifacts
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[21] Detection of S-nitrosothiols by fluorometric and colorimetric methods
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In vivo imaging of free radicals: Applications from mouse to man
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Coelenterazine Analogs as Chemiluminescent Probe for Superoxide Anion
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Myoglobin-Catalyzed Tyrosine Nitration: No Need for Peroxynitrite
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[20] Assay for superoxide dismutase based on chemiluminescence of luciferin analog
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Antioxidants and antioxidant methods: an updated overview
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Selective fluorescent imaging of superoxide <i>in vivo</i> using ethidium-based probes
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Mitochondrial dysfunction in cardiovascular disease
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Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity
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- Authors
- Tarpey, M. M., Wink, D. A., & Grisham, M. B.
- Affiliations
- University of Alabama at Birmingham (US)
- Venue
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Publisher
- American Physiological Society
- Published
- 2004-03
- Volume
- 286
- Issue
- 3
- Pages
- R431-R444
- ISSN
- 0363-6119 (ISSN-L)
- 1522-1490
- OpenAlex
- W2148883753
- Cited by
- 673
- References
- 153
- Citations by year
- 2026: 7 · 2025: 5 · 2024: 33 · 2023: 19 · 2022: 16 · 2021: 24 · 2020: 27 · 2019: 23 · 2018: 23 · 2017: 22 · 2016: 45 · 2015: 25 · 2014: 40 · 2013: 42 · 2012: 49
- FWCI
- 2.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hl066110-04)
- National Heart, Lung, and Blood Institute (HL-66110)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK64023, R01DK064023, P01DK043785, DK-43785)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Keywords
- reactive oxygen species, reactive nitrogen species, reactive metabolites, detection methods, in vivo measurement, in vitro measurement, antioxidant enzymes
- MeSH terms
- Algorithms, Animals, Humans, Hydrogen Peroxide, Nitric Oxide, Superoxides, Tyrosine, Oxidants, Reactive Oxygen Species, Oxidative Stress, Reactive Nitrogen Species, S-Nitrosothiols, Peroxynitrous Acid
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