The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Cantilever-Based Optical Deflection Assay for Discrimination of DNA Single-Nucleotide Mismatches
Abstract
Characterization of single-nucleotide polymorphisms is a major focus of current genomics research. We demonstrate the discrimination of DNA mismatches using an elegantly simple microcantilever-based optical deflection assay, without the need for external labeling. Gold-coated silicon AFM cantilevers were functionalized with thiolated 20- or 25-mer probe DNA oligonucleotides and exposed to target oligonucleotides of varying sequence in static and flow conditions. Hybridization of 10-mer complementary target oligonucleotides resulted in net positive deflection, while hybridization with targets containing one or two internal mismatches resulted in net negative deflection. Mismatched targets produced a stable and measurable signal when only a four-base pair stretch was complementary to the probe sequence. This technique is readily adaptable to a high-throughput array format and provides a distinct positive/negative signal for easy interpretation of oligonucleotide hybridization.
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Metadata
- Authors
- Hansen, K. M., Ji, H.-F., Wu, G., Datar, R., Cote, R., Majumdar, A., & Thundat, T.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-03-02
- Volume
- 73
- Issue
- 7
- Pages
- 1567-1571
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2038637564
- Cited by
- 337
- References
- 12
- FWCI
- 6.62
- Type
- journal article
- Language
- en
- Open access
- closed
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- Hansen, K. M., Ji, H.-F., Wu, G., Datar, R., Cote, R., Majumdar, A., & Thundat, T. (2001). Cantilever-Based Optical Deflection Assay for Discrimination of DNA Single-Nucleotide Mismatches. Analytical Chemistry. https://doi.org/10.1021/ac0012748
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