The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Effect of reducing disulfide-containing proteins on electrospray ionization mass spectra
Abstract
Electrospray ionization produces multiply charged molecular ions for biomolecules with molecular weights in excess of 100,000. This allows mass spectrometers with limited mass-to-charge range to extend their molecular weight range by a factor equal to the number of charges. The maximum number of observed charges for peptides and smaller proteins correlates well with the number of basic amino acid residues (Arg, Lys, His), except for disulfide-containing molecules, such as lysozyme and bovine albumin. However, reduction of disulfide linkages with 1,4-dithiothreitol (Cleland's reagent) may allow the protein to be in an extended conformation and make "buried" basic residues available for protonation to yield higher charged molecular ions by the electrospray ionization process. For larger proteins reduction of disulfide bridges greatly increases the maximum charge state, but charging of basic amino acid residues remains less efficient than for smaller proteins.
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Citations
Cites
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Electrospray ion source. Another variation on the free-jet theme
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Cited by
New developments in biochemical mass spectrometry: electrospray ionization
Smith, R. D., Loo, J. A., Edmonds, C. G., Barinaga, C. J., & Udseth, H. R. (1990) · Analytical Chemistry · doi:10.1021/ac00208a002 · Cited by 1,051
Self-citationProtein synthesis by native chemical ligation: Expanded scope by using straightforward methodology
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Self-citationProtein Complexes in the Gas Phase: Technology for Structural Genomics and Proteomics
Benesch, J. L. P., Ruotolo, B. T., Simmons, D. A., & Robinson, C. V. (2007) · Chemical Reviews · doi:10.1021/cr068289b · Cited by 408
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Self-citation
Metadata
- Authors
- Loo, J. A., Edmonds, C. G., Udseth, H. R., & Smith, R. D.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1990-04-01
- Volume
- 62
- Issue
- 7
- Pages
- 693-698
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2033210824
- Cited by
- 274
- References
- 32
- FWCI
- 11.35
- Type
- journal article
- Language
- en
- Open access
- closed
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- Loo, J. A., Edmonds, C. G., Udseth, H. R., & Smith, R. D. (1990). Effect of reducing disulfide-containing proteins on electrospray ionization mass spectra. Analytical Chemistry. https://doi.org/10.1021/ac00206a009
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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