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A Bacterial Method for the Nitrogen Isotopic Analysis of Nitrate in Seawater and Freshwater
Abstract
We report a new method for measurement of the isotopic composition of nitrate (NO3-) at the natural-abundance level in both seawater and freshwater. The method is based on the isotopic analysis of nitrous oxide (N20) generated from nitrate by denitrifying bacteria that lack N2O-reductase activity. The isotopic composition of both nitrogen and oxygen from nitrate are accessible in this way. In this first of two companion manuscripts, we describe the basic protocol and results for the nitrogen isotopes. The precision of the method is better than 0.2/1000 (1 SD) at concentrations of nitrate down to 1 microM, and the nitrogen isotopic differences among various standards and samples are accurately reproduced. For samples with 1 microM nitrate or more, the blank of the method is less than 10% of the signal size, and various approaches may reduce it further.
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Cited by
Santoro, A. E., Casciotti, K. L., & Francis, C. A. (2010) · Environmental Microbiology · doi:10.1111/j.1462-2920.2010.02205.x · Cited by 398
Self-citationCartigny, P., & Marty, B. (2013) · Elements · doi:10.2113/gselements.9.5.359 · Cited by 192
Nitrogen isotope dynamics of the Cariaco Basin, Venezuela
Thunell, R. C., Sigman, D. M., Muller‐Karger, F., Astor, Y., & Varela, R. (2004) · Global Biogeochemical Cycles · doi:10.1029/2003gb002185 · Cited by 185
Self-citationZhang, Y., Shi, P., Song, J., & Li, Q. (2018) · Applied Sciences · doi:10.3390/app9010018 · Cited by 121
Fate of effluent-borne contaminants beneath septic tank drainfields overlying a karst aquifer
Katz, B. G., Griffin, D. W., McMahon, P. B., Harden, H. S., Wade, E., Hicks, R. W., & Chanton, J. P. (2010) · Journal of Environmental Quality · doi:10.2134/jeq2009.0244 · Cited by 115
Hastings, M. G., Sigman, D. M., & Steig, E. J. (2005) · Global Biogeochemical Cycles · doi:10.1029/2005gb002502 · Cited by 73
Self-citationNitrogen in past marine environments
Galbraith, E. D., Sigman, D. M., Robinson, R. S., & Pedersen, T. F. (2008) · Nitrogen in the Marine Environment · doi:10.1016/b978-0-12-372522-6.00034-7 · Cited by 67
Self-citationTamborski, J. J., Cochran, J. K., & Bokuniewicz, H. J. (2017) · Geochimica et Cosmochimica Acta · doi:10.1016/j.gca.2017.09.003 · Cited by 58
Nitrate-driven anaerobic oxidation of ethane and butane by bacteria
Wu, M., Li, J., Lai, C.-Y., Leu, A. O., Sun, S., Gu, R., Erler, D. V., Liu, L., Li, L., Tyson, G. W., et al. (2024) · The ISME Journal · doi:10.1093/ismejo/wrad011 · Cited by 23
Kealoha, A. K., Shamberger, K. E. F., Shuler, C. K., McClain, S., Callender, T., & Sparks, R. (2025) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2025.118502
Metadata
- Authors
- Sigman, D. M., Casciotti, K. L., Andreani, M., Barford, C., Galanter, M., & Böhlke, J. K.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-07-27
- Volume
- 73
- Issue
- 17
- Pages
- 4145-4153
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2032292978
- Cited by
- 1,956
- References
- 0
- FWCI
- 14.70
- Type
- journal article
- Language
- en
- Open access
- closed
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