The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Correlation of Relative Abundance Ratios Derived from Peptide Ion Chromatograms and Spectrum Counting for Quantitative Proteomic Analysis Using Stable Isotope Labeling
Abstract
In this study, S. cerevisiae crude membrane fractions were prepared using the acid-labile detergent RapiGest from cells grown under rich and minimal media conditions using 14N and 15N ammonium sulfate as the sole nitrogen source. Four independent MudPIT analyses of 1:1 mixtures of sample were prepared and analyzed via quantitative multidimensional protein identification technology on a two-dimensional ion trap mass spectrometer. Using the method described in this study, low-abundance integral membrane proteins with up to 14 transmembrane domains were identified and their protein expression determined when sufficient spectrum counting and ion chromatogram information was generated. We demonstrate that spectrum counting and mass spectrometry derived ion chromatograms strongly correlate for determining quantitative changes in protein expression. Spectrum counting proved more reproducible and has a wider dynamic range contributing to the deviation of the two quantitative approaches from a perfect positive correlation.
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Large-scale analysis of the yeast proteome by multidimensional protein identification technology
Washburn, M. P., Wolters, D., & Yates, J. R. (2001) · Nature Biotechnology · doi:10.1038/85686 · Cited by 4,752
Self-citationMicroarray data normalization and transformation
Quackenbush, J. (2002) · Nature Genetics · doi:10.1038/ng1032 · Cited by 1,975
An Automated Multidimensional Protein Identification Technology for Shotgun Proteomics
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Self-citationAndersen, J. S., Lam, Y. W., Leung, A. K. L., Ong, S.-E., Lyon, C. E., Lamond, A. I., & Mann, M. (2005) · Nature · doi:10.1038/nature03207 · Cited by 1,149
Automated approach for quantitative analysis of complex peptide mixtures from tandem mass spectra
Venable, J. D., Dong, M.-Q., Wohlschlegel, J., Dillin, A., & Yates, J. R. (2004) · Nature Methods · doi:10.1038/nmeth705 · Cited by 794
Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics
Blagoev, B., Ong, S.-E., Kratchmarova, I., & Mann, M. (2004) · Nature Biotechnology · doi:10.1038/nbt1005 · Cited by 681
Quantitative Proteomic Analysis by Accurate Mass Retention Time Pairs
Silva, J. C., Denny, R., Dorschel, C. A., Gorenstein, M., Kass, I. J., Li, G.-Z., McKenna, T., Nold, M. J., Richardson, K., Young, P., et al. (2005) · Analytical Chemistry · doi:10.1021/ac048455k · Cited by 639
Metabolic labeling of C. elegans and D. melanogaster for quantitative proteomics
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Proteogenomic characterization of human colon and rectal cancer
CPTAC, T. N., Zhang, B., Wang, J., Wang, X., Zhu, J., Liu, Q., Shi, Z., Chambers, M. C., Zimmerman, L. J., Shaddox, K. F., et al. (2014) · Nature · doi:10.1038/nature13438 · Cited by 1,462
Proteomics by Mass Spectrometry: Approaches, Advances, and Applications
Yates, J. R., Ruse, C. I., & Nakorchevsky, A. (2009) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-061008-124934 · Cited by 1,078
Mass Spectrometry-Based Label-Free Quantitative Proteomics
Zhu, W., Smith, J. W., & Huang, C.-M. (2010) · Journal of Biomedicine and Biotechnology · doi:10.1155/2010/840518 · Cited by 629
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Metadata
- Authors
- Zybailov, B., Coleman, M. K., Florens, L., & Washburn, M. P.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-08-31
- Volume
- 77
- Issue
- 19
- Pages
- 6218-6224
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2065436349
- Cited by
- 349
- References
- 23
- FWCI
- 10.55
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zybailov, B., Coleman, M. K., Florens, L., & Washburn, M. P. (2005). Correlation of Relative Abundance Ratios Derived from Peptide Ion Chromatograms and Spectrum Counting for Quantitative Proteomic Analysis Using Stable Isotope Labeling. Analytical Chemistry. https://doi.org/10.1021/ac050846r
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