The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Single-Cell Enzyme Concentrations, Kinetics, and Inhibition Analysis Using High-Density Hydrodynamic Cell Isolation Arrays
Abstract
High-quality single-cell data are required for a quantitative systems biology description of cellular function. However, data of this type are difficult and time-consuming to collect using traditional techniques. We present a robust and simple microfluidic method for trapping single cells in large arrays to address this problem. Ordered single-cell isolation arrays allow for high-density microscopic analysis with simplified image processing. Moreover, for fluorescent assays, on-chip sample preparation (e.g., fluorescent labeling, washing) can be performed, as opposed to manual intensive operations of incubation, centrifugation, and resuspension in previous techniques-saving time and reagents. This technology was applied to determine novel single-cell enzyme kinetics for three different cell types (HeLa, 293T, Jurkat). A kinetic model of this process predicted this varied response was due to variation in the concentration of carboxylesterase between cell types. Nordihydroguaiaretic acid (NDGA) was also characterized as an inhibitor of carboxylesterases. For HeLa cells, 20 nM of the 50 nM total carboxylesterases was unaffected by NDGA. This type of analysis could be directly applied to quantify a variety of intracellular enzymes with available fluorogenic substrates.
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Wolfe, A. J. (2005) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.69.1.12-50.2005 · Cited by 1,233
THE MAMMALIAN CARBOXYLESTERASES: From Molecules to Functions
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Characterization of Photodamage to Escherichia coli in Optical Traps
Neuman, K. C., Chadd, E. H., Liou, G. F., Bergman, K., & Block, S. M. (1999) · Biophysical Journal · doi:10.1016/s0006-3495(99)77117-1 · Cited by 687
Evolution of Fluorescein as a Platform for Finely Tunable Fluorescence Probes
Urano, Y., Kamiya, M., Kanda, K., Ueno, T., Hirose, K., & Nagano, T. (2005) · Journal of the American Chemical Society · doi:10.1021/ja043919h · Cited by 683
Microfluidic Device for Single-Cell Analysis
Wheeler, A. R., Throndset, W. R., Whelan, R. J., Leach, A. M., Zare, R. N., Liao, Y. H., Farrell, K., Manger, I. D., & Daridon, A. (2003) · Analytical Chemistry · doi:10.1021/ac0340758 · Cited by 600
Large-Scale Single-Cell Trapping And Imaging Using Microwell Arrays
Rettig, J. R., & Folch, A. (2005) · Analytical Chemistry · doi:10.1021/ac0505977 · Cited by 502
A Microfabrication-Based Dynamic Array Cytometer
Voldman, J., Gray, M. L., Toner, M., & Schmidt, M. A. (2002) · Analytical Chemistry · doi:10.1021/ac0256235 · Cited by 328
Revzin, A., Sekine, K., Sin, A., Tompkins, R. G., & Toner, M. (2005) · Lab on a Chip · doi:10.1039/b405557h · Cited by 175
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Structural insights into CPT-11 activation by mammalian carboxylesterases
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Aung, A., Kumar, V., Theprungsirikul, J., Davey, S. K., & Varghese, S. (2020) · Cancer Research · doi:10.1158/0008-5472.can-19-0342 · Cited by 146
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(2022) · Progress in Molecular Biology and Translational Science · doi:10.1016/bs.pmbts.2021.07.022 · Cited by 23
Metadata
- Authors
- Di Carlo, D., Aghdam, N., & Lee, L. P.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-05-25
- Volume
- 78
- Issue
- 14
- Pages
- 4925-4930
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2010477093
- Cited by
- 458
- References
- 14
- FWCI
- 20.96
- Type
- journal article
- Language
- en
- Open access
- closed
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- Di Carlo, D., Aghdam, N., & Lee, L. P. (2006). Single-Cell Enzyme Concentrations, Kinetics, and Inhibition Analysis Using High-Density Hydrodynamic Cell Isolation Arrays. Analytical Chemistry. https://doi.org/10.1021/ac060541s
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