The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Anti-DNA:RNA Antibodies and Silicon Photonic Microring Resonators: Increased Sensitivity for Multiplexed microRNA Detection
Open accessAbstract
In this paper, we present a method for the sensitive detection of microRNAs (miRNAs) utilizing an antibody that specifically recognizes DNA:RNA heteroduplexes and a silicon photonic microring resonator array transduction platform. Microring resonator arrays are covalently functionalized with DNA capture probes that are complementary to solution phase miRNA targets. Following hybridization on the sensor, the anti-DNA:RNA antibody is introduced and binds selectively to the heteroduplexes, giving a larger signal than the original miRNA hybridization due to the increased mass of the antibody, as compared to the 22-mer oligoribonucleotide. Furthermore, the secondary recognition step is performed in neat buffer solution and at relatively higher antibody concentrations, facilitating the detection of miRNAs of interest. The intrinsic sensitivity of the microring resonator platform coupled with the amplification provided by the anti-DNA:RNA antibodies allows for the detection of microRNAs at concentrations as low as 10 pM (350 amol). The simplicity and sequence generality of this amplification method position it as a promising tool for high-throughput, multiplexed miRNA analysis as well as a range of other RNA based detection applications.
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Cites
Most mammalian mRNAs are conserved targets of microRNAs
Friedman, R. C., Farh, K. K.-H., Burge, C. B., & Bartel, D. P. (2009) · Genome Research · doi:10.1101/gr.082701.108 · Cited by 8,462
A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing
Landgraf, P., Rusu, M., Sheridan, R., Sewer, A., Iovino, N., Aravin, A., Pfeffer, S., Rice, A., Kamphorst, A. O., Landthaler, M., et al. (2007) · Cell · doi:10.1016/j.cell.2007.04.040 · Cited by 3,626
A pancreatic islet-specific microRNA regulates insulin secretion
Poy, M. N., Eliasson, L., Krutzfeldt, J., Kuwajima, S., Ma, X., MacDonald, P. E., Pfeffer, S., Tuschl, T., Rajewsky, N., Rorsman, P., et al. (2004) · Nature · doi:10.1038/nature03076 · Cited by 2,102
A brain-specific microRNA regulates dendritic spine development
Schratt, G. M., Tuebing, F., Nigh, E. A., Kane, C. G., Sabatini, M. E., Kiebler, M., & Greenberg, M. E. (2006) · Nature · doi:10.1038/nature04367 · Cited by 1,786
Sempere, L. F., Freemantle, S., Pitha-Rowe, I., Moss, E., Dmitrovsky, E., & Ambros, V. (2004) · Genome Biology · doi:10.1186/gb-2004-5-3-r13 · Cited by 1,578
Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
Chiang, H. R., Schoenfeld, L. W., Ruby, J. G., Auyeung, V. C., Spies, N., Baek, D., Johnston, W. K., Russ, C., Luo, S., Babiarz, J. E., et al. (2010) · Genes & Development · doi:10.1101/gad.1884710 · Cited by 825
Probing microRNAs with microarrays: tissue specificity and functional inference
BABAK, T., ZHANG, W., MORRIS, Q., BLENCOWE, B. J., & HUGHES, T. R. (2004) · RNA · doi:10.1261/rna.7119904 · Cited by 385
Cited by
MicroRNA profiling: approaches and considerations
Pritchard, C. C., Cheng, H. H., & Tewari, M. (2012) · Nature Reviews Genetics · doi:10.1038/nrg3198 · Cited by 1,765
Jiang, X., Qavi, A. J., Huang, S. H., & Yang, L. (2020) · Matter · doi:10.1016/j.matt.2020.07.008 · Cited by 353
Self-citationKilic, T., Erdem, A., Ozsoz, M., & Carrara, S. (2018) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2017.08.007 · Cited by 299
High-Q Optical Sensors for Chemical and Biological Analysis
Luchansky, M. S., & Bailey, R. C. (2012) · Analytical Chemistry · doi:10.1021/ac2029024 · Cited by 288
Self-citationBiofunctionalization of Multiplexed Silicon Photonic Biosensors
Puumala, L. S., Grist, S. M., Morales, J. M., Bickford, J. R., Chrostowski, L., Shekhar, S., & Cheung, K. C. (2022) · Biosensors · doi:10.3390/bios13010053 · Cited by 55
Metadata
- Authors
- Qavi, A. J., Kindt, J. T., Gleeson, M. A., & Bailey, R. C.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-06-29
- Volume
- 83
- Issue
- 15
- Pages
- 5949-5956
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1984303885
- Cited by
- 158
- References
- 7
- FWCI
- 5.29
- Type
- journal article
- Language
- en
- Open access
- green
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