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Cited by 61Photonic and Optical DevicesAdvanced Biosensing Techniques and ApplicationsMicrofluidic and Capillary Electrophoresis Applications

The DOI matches its Crossref record; 315 of the 315 references it cites were found in a registry or an index.

Biofunctionalization of Multiplexed Silicon Photonic Biosensors

Open access
Puumala, Lauren S., Grist, Samantha M., Morales, Jennifer M., Bickford, Justin R., Chrostowski, Lukas, Shekhar, Sudip, Cheung, Karen C.·2022·Biosensors
DOI 10.3390/bios13010053PMC PMC9855810
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Abstract

Silicon photonic (SiP) sensors offer a promising platform for robust and low-cost decentralized diagnostics due to their high scalability, low limit of detection, and ability to integrate multiple sensors for multiplexed analyte detection. Their CMOS-compatible fabrication enables chip-scale miniaturization, high scalability, and low-cost mass production. Sensitive, specific detection with silicon photonic sensors is afforded through biofunctionalization of the sensor surface; consequently, this functionalization chemistry is inextricably linked to sensor performance. In this review, we first highlight the biofunctionalization needs for SiP biosensors, including sensitivity, specificity, cost, shelf-stability, and replicability and establish a set of performance criteria. We then benchmark biofunctionalization strategies for SiP biosensors against these criteria, organizing the review around three key aspects: bioreceptor selection, immobilization strategies, and patterning techniques. First, we evaluate bioreceptors, including antibodies, aptamers, nucleic acid probes, molecularly imprinted polymers, peptides, glycans, and lectins. We then compare adsorption, bioaffinity, and covalent chemistries for immobilizing bioreceptors on SiP surfaces. Finally, we compare biopatterning techniques for spatially controlling and multiplexing the biofunctionalization of SiP sensors, including microcontact printing, pin- and pipette-based spotting, microfluidic patterning in channels, inkjet printing, and microfluidic probes.

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Crossref · 10.3390/bios13010053

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  • After the oxide-open step, functionalization of the resonator surface significantly depends on waveguide design and affinity.

    “After the oxide-open step, functionalization of the resonator surface significantly depends on waveguide design and affinity156.”

    From Roadmapping the next generation of silicon photonics (2024) · cites this paper as [156]

    The Introduction says biofunctionalization strategies should suit the sensor architecture and that bioreceptors should have high affinity for the target analyte. The covalent-immobilization section also describes chemistry that selectively functionalizes silicon nitride waveguides rather than surrounding oxide. Our copy does not establish the claimed sequence after an “oxide-open step,” and its discussion of affinity concerns bioreceptor–analyte binding, not affinity of the resonator surface. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

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Citations

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Research works printed but not linked: 5

  1. Kumar, D., Prasad, M., and Mohan, H. (2022). Biological recognition elements. Electrochemical Sensors, Elsevier.
  2. Abbas MBBS, A.K., Lichtman MD PhD, A.H., and Pillai MBBS PhD, S. (2021). Cellular and Molecular Immunology, Elsevier. [10th ed.].
  3. Varki, A., Cummings, R.D., Esko, J.D., Stanley, P., Hart, G.W., Aebi, M., Darvill, A.G., Kinoshita, T., Packer, N.H., and Prestegard, J.H. (2015). Chapter 53: Chemical synthesis of glycans and glycoconjugates. Essentials of Glycobiology, Cold Spring Harbor Laboratory Press.
  4. Varki, A., Cummings, R.D., Esko, J.D., Stanley, P., Hart, G.W., Aebi, M., Darvill, A.G., Kinoshita, T., Packer, N.H., and Prestegard, J.H. (2015). Structural analysis of glycans. Essentials of Glycobiology, Cold Spring Harbor Laboratory Press.
  5. Varki, A., Cummings, R.D., Esko, J.D., Stanley, P., Hart, G.W., Aebi, M., Darvill, A.G., Kinoshita, T., Packer, N.H., and Prestegard, J.H. (2015). Chapter 54: Chemoenzymatic synthesis of glycans and glycoconjugates. Essentials of Glycobiology, Cold Spring Harbor Laboratory Press.

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Metadata

Authors
Puumala, L. S., Grist, S. M., Morales, J. M., Bickford, J. R., Chrostowski, L., Shekhar, S., & Cheung, K. C.
Affiliations
  • University of British Columbia (CA)
  • DEVCOM Army Research Laboratory (US)
Corresponding author
  • Puumala, Lauren S.
  • Cheung, Karen C.
ORCID
Venue
Biosensors
Publisher
MDPI AG
Published
2022-12-29
Volume
13
Issue
1
Pages
53
ISSN
2079-6374 (ISSN-L)
DOI
10.3390/bios13010053
PMC
PMC9855810
OpenAlex
W4313362517
Cited by
55
References
315
Citations by year
2026: 16 · 2025: 16 · 2024: 16 · 2023: 7
FWCI
0.64
Type
journal article
Language
en
Open access
Licence
Locations
  • Biosensors (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • cIRcle (University of British Columbia) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Canada’s Digital Technology Supercluster (IT25501)
  • Schmidt Futures program (IT25501)
  • MITACS, Inc./Elevate Postdoctoral Fellowship Program (IT25501)
Topics
  • Physical Sciences › Engineering › Electrical and Electronic Engineering › Photonic and Optical Devices
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Keywords
biofunctionalization, bioreceptor immobilization, aptamers, immunoglobulins, nucleic acid probes, molecularly imprinted polymers, lectins, covalent immobilization, adsorption, microcontact printing, inkjet printing, microfluidic patterning, decentralized diagnostics, CMOS-compatible photonics
MeSH terms
Antibodies, Silicon, Biosensing Techniques, Lectins, Optics and Photonics

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