The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification
Open accessAbstract
Droplet digital polymerase chain reaction (ddPCR) is a new technology that was recently commercialized to enable the precise quantification of target nucleic acids in a sample. ddPCR measures absolute quantities by counting nucleic acid molecules encapsulated in discrete, volumetrically defined, water-in-oil droplet partitions. This novel ddPCR format offers a simple workflow capable of generating highly stable partitioning of DNA molecules. In this study, we assessed key performance parameters of the ddPCR system. A linear ddPCR response to DNA concentration was obtained from 0.16% through to 99.6% saturation in a 20,000 droplet assay corresponding to more than 4 orders of magnitude of target DNA copy number per ddPCR. Analysis of simplex and duplex assays targeting two distinct loci in the Lambda DNA genome using the ddPCR platform agreed, within their expanded uncertainties, with values obtained using a lower density microfluidic chamber based digital PCR (cdPCR). A relative expanded uncertainty under 5% was achieved for copy number concentration using ddPCR. This level of uncertainty is much lower than values typically observed for quantification of specific DNA target sequences using currently commercially available real-time and digital cdPCR technologies.
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Cites
High-throughput droplet digital PCR system for absolute quantitation of DNA copy number
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Cited by
Absolute quantification by droplet digital PCR versus analog real-time PCR
Hindson, C. M., Chevillet, J. R., Briggs, H. A., Gallichotte, E. N., Ruf, I. K., Hindson, B. J., Vessella, R. L., & Tewari, M. (2013) · Nature Methods · doi:10.1038/nmeth.2633 · Cited by 1,488
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“This is not currently possible with the dPCR approach used here; however, with the development of higher volume and throughput dPCR methods this option will also be possible (35–37) with comparable accuracy (38).”
MethodChimeric antigen receptor natural killer (CAR-NK) cell design and engineering for cancer therapy
Gong, Y., Klein Wolterink, R. G. J., Wang, J., Bos, G. M. J., & Germeraad, W. T. V. (2021) · Journal of Hematology & Oncology · doi:10.1186/s13045-021-01083-5 · Cited by 379
Metadata
- Authors
- Pinheiro, L. B., Coleman, V. A., Hindson, C. M., Herrmann, J., Hindson, B. J., Bhat, S., & Emslie, K. R.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-12-21
- Volume
- 84
- Issue
- 2
- Pages
- 1003-1011
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2113559596
- Cited by
- 1,055
- References
- 5
- FWCI
- 11.52
- Type
- journal article
- Language
- en
- Open access
- hybrid
- Funders
- National Institute of Biomedical Imaging and Bioengineering
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