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Cited by 168

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Multiplex Detection of Protease Activity with Quantum Dot Nanosensors Prepared by Intein-Mediated Specific Bioconjugation

Open access
Xia, Zuyong, Xing, Yun, So, Min-Kyung, Koh, Ai Leen, Sinclair, Robert, Rao, Jianghong·2008·Analytical Chemistry
DOI 10.1021/ac801562f
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Abstract

We report here a protease sensing nanoplatform based on semiconductor nanocrystals or quantum dots (QDs) and bioluminescence resonance energy transfer (QD-BRET) to detect the protease activity in complex biological samples. These nanosensors consist of bioluminescent proteins as the BRET donor, quantum dots as the BRET acceptor, and protease substrates sandwiched between the two as a sensing group. An intein-mediated conjugation strategy was developed for site-specific conjugation of proteins to QDs in preparing these QD nanosensors. In this traceless ligation, the intein itself is spliced out and excluded from the final conjugation product. With this method, we have synthesized a series of QD nanosensors for highly sensitive detection of an important class of protease matrix metalloproteinase (MMP) activity. We demonstrated that these nanosensors can detect the MMP activity in buffers and in mouse serum with the sensitivity to a few nanograms per milliliter and secreted proteases by tumor cells. The suitability of these nanosensors for a multiplex protease assay has also been shown.

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Research works printed but not linked: 44

  1. Fingleton B., 2006, Front. Biosci., 11, 479, 10.2741/1811 · DOI · Paper page
  2. Brinckerhoff C. E., 2002, Nat. Rev. Mol. Cell. Biol., 3, 207, 10.1038/nrm763 · DOI · Paper page
  3. Bachmeier B. E., 2005, Expert Rev. Anticancer Ther., 5, 149, 10.1586/14737140.5.1.149 · DOI
  4. Overall C. M., 2006, Nat. Rev. Cancer, 6, 227, 10.1038/nrc1821 · DOI · Paper page
  5. Murphy G., 1994, J. Biol. Chem., 269, 6632, 10.1016/S0021-9258(17)37419-7 · DOI · Paper page
  6. Knight C. G., 1992, FEBS Lett., 296, 263, 10.1016/0014-5793(92)80300-6 · DOI · Paper page
  7. Pham W., 2004, Bioconjugate Chem., 15, 1403, 10.1021/bc049924s · DOI
  8. McIntyre J. O., 2004, Biochem. J., 377, 617, 10.1042/bj20030582 · DOI · Paper page
  9. Bremer C., 2001, Nat. Med., 7, 743, 10.1038/89126 · DOI · Paper page
  10. Zhao M., 2003, Angew. Chem., Int. Ed., 42, 1375, 10.1002/anie.200390352 · DOI · Paper page
  11. Harris T. J., 2006, Angew. Chem., Int. Ed., 45, 3161, 10.1002/anie.200600259 · DOI
  12. Lepage M., 2007, Mol. Imaging, 6, 393, 10.2310/7290.2007.00035 · doi:10.2310/7290.2007.00035
  13. Bruchez M., 1998, Science, 281, 2013, 10.1126/science.281.5385.2013 · DOI · Paper page
  14. Michalet X., 2005, Science, 307, 538, 10.1126/science.1104274 · DOI · Paper page
  15. Medintz I. L., 2005, Nat. Mater., 4, 435, 10.1038/nmat1390 · DOI · Paper page
  16. Katz E., 2004, Angew. Chem., Int. Ed., 43, 6042, 10.1002/anie.200400651 · DOI · Paper page
  17. Daniel M.-C., 2004, Chem. Rev., 104, 293, 10.1021/cr030698+ · DOI · Paper page
  18. Alivisatos P., 2004, Nat. Biotechnol., 22, 47, 10.1038/nbt927 · DOI · Paper page
  19. Medintz I. L., 2004, Proc. Natl. Acad. Sci. U.S.A., 101, 9612, 10.1073/pnas.0403343101 · DOI · Paper page
  20. Medinitz I. L., 2003, Nat. Mater., 2, 630, 10.1038/nmat961 · DOI · Paper page
  21. Somers R. C., 2007, Chem. Soc. Rev., 36, 579, 10.1039/b517613c · DOI · Paper page
  22. Gao X., 2005, Curr. Opin. Biotechnol., 16, 63, 10.1016/j.copbio.2004.11.003 · DOI · Paper page
  23. Rosenthal S. J., 2008, J. Am. Chem. Soc., 130, 5720, 10.1021/ja710870e · DOI · Paper page
  24. Suzuki M., 2008, J. Am. Chem. Soc., 130, 5720, 10.1021/ja710870e · DOI · Paper page
  25. Shi L., 2006, J. Am. Chem. Soc., 128, 10378, 10.1021/ja063509o · DOI · Paper page
  26. Kim Y.-P., 2008, Anal. Chem., 80, 4634, 10.1021/ac702416e · doi:10.1021/ac702416e
  27. Troy T., 2004, Mol. Imaging, 3, 9, 10.1162/153535004773861688 · DOI · Paper page
  28. Contag P. R., 1998, Nat. Med., 4, 245, 10.1038/nm0298-245 · DOI · Paper page
  29. Contag C. H., 2002, Annu. Rev. Biomed. Eng., 4, 35, 10.1146/annurev.bioeng.4.111901.093336 · DOI · Paper page
  30. So M.-K., 2006, Nat. Biotechnol., 24, 339, 10.1038/nbt1188 · DOI · Paper page
  31. So M.-K., 2006, Nat. Protocols, 1, 1160, 10.1038/nprot.2006.162 · DOI
  32. Evans T. C., 2002, Chem. Rev., 102, 4869, 10.1021/cr9601369 · DOI
  33. Muralidharan V., 2006, Nat. Methods, 3, 429, 10.1038/nmeth886 · DOI · Paper page
  34. Muir T. W., 2003, Annu. Rev. Biochem., 72, 249, 10.1146/annurev.biochem.72.121801.161900 · DOI · Paper page
  35. Liu X. Q., 2000, Annu. Rev. Genetics, 34, 61, 10.1146/annurev.genet.34.1.61 · DOI
  36. Loening A. M, 2006, Protein Eng., Des. Sel., 19, 391, 10.1093/protein/gzl023 · DOI · Paper page
  37. Turk B. E., 2001, Nat. Biotechnol., 19, 661, 10.1038/90273 · DOI · Paper page
  38. Telenti A., 1997, J. Bacteriol., 179, 6378, 10.1128/jb.179.20.6378-6382.1997 · doi:10.1128/jb.179.20.6378-6382.1997
  39. Kalia J., 2006, ChemBioChem, 7, 1375, 10.1002/cbic.200600150 · DOI
  40. Law B., 2004, Chem. Biol., 11, 99, 10.1016/j.chembiol.2003.12.017 · DOI
  41. Giambernardi T. A., 1998, Matrix Biol., 16, 483, 10.1016/S0945-053X(98)90019-1 · DOI · Paper page
  42. Stahtea X. N., 2007, Int. J. Cancer, 121, 2808, 10.1002/ijc.23029 · DOI
  43. Zhang Y., 2006, Nano Lett., 6, 1988, 10.1021/nl0611586 · DOI
  44. Zhang Y., 2006, Angew. Chem., Int. Ed., 45, 4936, 10.1002/anie.200601197 · DOI · Paper page

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Metadata

Authors
Xia, Z., Xing, Y., So, M.-K., Koh, A. L., Sinclair, R., & Rao, J.
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
2008-10-16
Volume
80
Issue
22
Pages
8649-8655
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
DOI
10.1021/ac801562f
OpenAlex
W2015859382
Cited by
168
References
2
FWCI
7.58
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Multiplex Detection of Protease Activity with Quantum Dot Nanosensors Prepared by Intein-Mediated Specific Bioconjugation. https://app.cochat.ai/papers/10.1021/ac801562f
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Xia, Z., Xing, Y., So, M.-K., Koh, A. L., Sinclair, R., & Rao, J. (2008). Multiplex Detection of Protease Activity with Quantum Dot Nanosensors Prepared by Intein-Mediated Specific Bioconjugation. Analytical Chemistry. https://doi.org/10.1021/ac801562f

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