The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Droplet-Based Microfluidic Systems for High-Throughput Single DNA Molecule Isothermal Amplification and Analysis
Abstract
We have developed a method for high-throughput isothermal amplification of single DNA molecules in a droplet-based microfluidic system. DNA amplification in droplets was analyzed using an intercalating fluorochrome, allowing fast and accurate "digital" quantification of the template DNA based on the Poisson distribution of DNA molecules in droplets. The clonal amplified DNA in each 2 pL droplet was further analyzed by measuring the enzymatic activity of the encoded proteins after fusion with a 15 pL droplet containing an in vitro translation system.
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Citations
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Cited by
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Transient compartmentalization of RNA replicators prevents extinction due to parasites
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Self-citation
Metadata
- Authors
- Mazutis, L., Araghi, A. F., Miller, O. J., Baret, J.-C., Frenz, L., Janoshazi, A., Taly, V., Miller, B. J., Hutchison, J. B., Link, D., Griffiths, A. D., & Ryckelynck, M.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-05-18
- Volume
- 81
- Issue
- 12
- Pages
- 4813-4821
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2074905581
- Cited by
- 263
- References
- 26
- FWCI
- 12.23
- Type
- journal article
- Language
- en
- Open access
- closed
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