Digital PCR on a SlipChip
Open accessAbstract
This paper describes a SlipChip to perform digital PCR in a very simple and inexpensive format. The fluidic path for introducing the sample combined with the PCR mixture was formed using elongated wells in the two plates of the SlipChip designed to overlap during sample loading. This fluidic path was broken up by simple slipping of the two plates that removed the overlap among wells and brought each well in contact with a reservoir preloaded with oil to generate 1280 reaction compartments (2.6 nL each) simultaneously. After thermal cycling, end-point fluorescence intensity was used to detect the presence of nucleic acid. Digital PCR on the SlipChip was tested quantitatively by using Staphylococcus aureus genomic DNA. As the concentration of the template DNA in the reaction mixture was diluted, the fraction of positive wells decreased as expected from the statistical analysis. No cross-contamination was observed during the experiments. At the extremes of the dynamic range of digital PCR the standard confidence interval determined using a normal approximation of the binomial distribution is not satisfactory. Therefore, statistical analysis based on the score method was used to establish these confidence intervals. The SlipChip provides a simple strategy to count nucleic acids by using PCR. It may find applications in research applications such as single cell analysis, prenatal diagnostics, and point-of-care diagnostics. SlipChip would become valuable for diagnostics, including applications in resource-limited areas after integration with isothermal nucleic acid amplification technologies and visual readout.
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SlipChip is especially appropriate for integrated DNA-based data storage because all microdroplets can be moved simultaneously by sliding, so no valves, pumps, or tubes are required.
“As a microfluidic device for handling a large array of microdroplets, SlipChip is a platform that is especially appropriate for the development of an integrated DNA-based data storage system. All the microdroplets can be moved simultaneously on a SlipChip by simply sliding the device, so no valves, pumps, or tubes are required (54–56).”
From Electrochemical DNA synthesis and sequencing on a single electrode with scalability for integrated data storage (2021) · cites this paper as [56]
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- Authors
- Shen, F., Du, W., Kreutz, J. E., Fok, A., & Ismagilov, R. F.
- Affiliations
- University of Chicago (US)
- Corresponding author
- Ismagilov, Rustem F.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2010
- Volume
- 10
- Issue
- 20
- Pages
- 2666
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- PMC
- PMC2948063
- OpenAlex
- W2150204651
- Cited by
- 295
- References
- 59
- Citations by year
- 2026: 14 · 2025: 14 · 2024: 16 · 2023: 14 · 2022: 19 · 2021: 16 · 2020: 24 · 2019: 23 · 2018: 15 · 2017: 17 · 2016: 21 · 2015: 18 · 2014: 20 · 2013: 21 · 2012: 19
- FWCI
- 12.11
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Lab on a Chip (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- Funders
- National Science Foundation
- National Institutes of Health (DP1OD003584)
- Office of Naval Research (N00014)
- National Institute of General Medical Sciences
- Division of Materials Research
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrowetting and Microfluidic Technologies
- Keywords
- digital PCR, SlipChip, microfluidics, droplet formation, fluorescence detection, DNA quantification, scoring methods, confidence intervals, low-cost diagnostics, point-of-care testing, resource-limited settings
- MeSH terms
- Chromosome Mapping, DNA, Bacterial, Equipment Design, Microscopy, Fluorescence, Signal Processing, Computer-Assisted, Staphylococcus aureus, Polymerase Chain Reaction, Equipment Failure Analysis, Microfluidic Analytical Techniques
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