The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
In Situ Surface Plasmon Resonance Imaging Detection of DNA Hybridization to Oligonucleotide Arrays on Gold Surfaces
Abstract
A new method for constructing oligonucleotide arrays on gold surfaces has been developed, and these arrays have been used in DNA hybridization experiments with in situ surface plasmon resonance (SPR) imaging detection. The detection technique was able to differentiate between single- and double-stranded DNA regions on the gold surface. The hybridization of both oligonucleotides and PCR-amplified DNA fragments was detectable, with the latter exhibiting slower hybridization kinetics. Temperature control of the in situ SPR cell was used to discriminate between perfectly matched duplexes and single-base-mismatched duplexes. The SPR detection technique requires no label on the DNA, but fluorescently labeled targets were also tested and detected by fluorescence imaging as an independent verification of the hybridization behavior of these DNA arrays. The in situ SPR imaging method for detection of DNA hybridization is expected to complement other existing methods for study of DNA interactions and might find future uses in mutation screening assays and DNA resequencing.
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Cites
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Cited by
A Survey of Structure−Property Relationships of Surfaces that Resist the Adsorption of Protein
Ostuni, E., Chapman, R. G., Holmlin, R. E., Takayama, S., & Whitesides, G. M. (2001) · Langmuir · doi:10.1021/la010384m · Cited by 1,799
Colloidal Au-Enhanced Surface Plasmon Resonance for Ultrasensitive Detection of DNA Hybridization
He, L., Musick, M. D., Nicewarner, S. R., Salinas, F. G., Benkovic, S. J., Natan, M. J., & Keating, C. D. (2000) · Journal of the American Chemical Society · doi:10.1021/ja001215b · Cited by 932
Quantitative multispectral biosensing and 1D imaging using quasi-3D plasmonic crystals
Stewart, M. E., Mack, N. H., Malyarchuk, V., Soares, J. A. N. T., Lee, T.-W., Gray, S. K., Nuzzo, R. G., & Rogers, J. A. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0606216103 · Cited by 340
Adsorption of Proteins to Hydrophobic Sites on Mixed Self-Assembled Monolayers
Ostuni, E., Grzybowski, B. A., Mrksich, M., Roberts, C. S., & Whitesides, G. M. (2003) · Langmuir · doi:10.1021/la020649c · Cited by 261
CHORLEY, B., WANG, X., CAMPBELL, M., PITTMAN, G., NOUREDDINE, M., & BELL, D. (2008) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2008.05.001 · Cited by 177
Metadata
- Authors
- Thiel, A. J., Frutos, A. G., Jordan, C. E., Corn, R. M., & Smith, L. M.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1997-12-01
- Volume
- 69
- Issue
- 24
- Pages
- 4948-4956
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2054386813
- Cited by
- 269
- References
- 31
- FWCI
- 2.70
- Type
- journal article
- Language
- en
- Open access
- closed
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