The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/Gravitational Field-Flow Fractionation
Abstract
Dielectrophoretic/gravitational field-flow fractionation (DEP/G-FFF) was used to separate cultured human breast cancer MDA-435 cells from normal blood cells mixed together in a sucrose/dextrose medium. An array of microfabricated, interdigitated electrodes of 50 microns widths and spacings, and lining the bottom surface of a thin chamber (0.42 mm H x 25 mm W x 300 mm L), was used to generate DEP forces that levitated the cells. A 10-microL cell mixture sample containing approximately 50,000 cells was introduced into the chamber, and cancerous and normal blood cells were levitated to different heights according to the balance of DEP and gravitational forces. The cells at different heights were transported at different velocities under the influence of a parabolic flow profile that was established in the chamber and were thereby separated. Separation performance depended on the frequency and voltage of the applied DEP field and the fluid-flow rate. It took as little as 5 min to achieve cell separation. An analysis of the DEP/G-FFF results revealed that the separation exploited the difference in dielectric and density properties between cell populations. The DEP/G-FFF technique is potentially applicable to many biological and biomedical problems, especially those related to microfluidic systems.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Goycoolea, A. (1997) · Cited by 3,615
Jones, T. B. (1995) · doi:10.1017/cbo9780511574498 · Cited by 2,786
Pethig, R., & Kell, D. B. (1987) · Physics in Medicine and Biology · doi:10.1088/0031-9155/32/8/001 · Cited by 796
Long-term human breast carcinoma cell lines of metastatic origin: Preliminary characterization
Cailleau, R., Olivé, M., & Cruciger, Q. V. J. (1978) · In Vitro · doi:10.1007/bf02616120 · Cited by 679
Flow Cytometry: A Practical Approach
Ormerod, M. G. (1994) · Cited by 452
Dielectrophoretic Sorting of Particles and Cells in a Microsystem
Fiedler, S., Shirley, S. G., Schnelle, T., & Fuhr, G. (1998) · Analytical Chemistry · doi:10.1021/ac971063b · Cited by 432
Microsystem Technology in Chemistry and Life Science
(1998) · Topics in Current Chemistry · doi:10.1007/3-540-69544-3 · Cited by 347
Cheng, J., Sheldon, E. L., Wu, L., Uribe, A., Gerrue, L. O., Carrino, J., Heller, M. J., & O'Connell, J. P. (1998) · Nature Biotechnology · doi:10.1038/nbt0698-541 · Cited by 347
Pethig, R., Huang, Y., Wang, X.-B., & Burt, J. P. H. (1992) · Journal of Physics D: Applied Physics · doi:10.1088/0022-3727/25/5/022 · Cited by 312
Self-citationIntroducing dielectrophoresis as a new force field for field-flow fractionation
Huang, Y., Wang, X. B., Becker, F. F., & Gascoyne, P. R. (1997) · Biophysical Journal · doi:10.1016/s0006-3495(97)78144-x · Cited by 302
Self-citation
Cited by
Toner, M., & Irimia, D. (2005) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.7.011205.135108 · Cited by 623
Adams, A. A., Okagbare, P. I., Feng, J., Hupert, M. L., Patterson, D., Göttert, J., McCarley, R. L., Nikitopoulos, D., Murphy, M. C., & Soper, S. A. (2008) · Journal of the American Chemical Society · doi:10.1021/ja8015022 · Cited by 521
Lab-on-a-chip for drug development
Weigl, B. H., Bardell, R. L., & Cabrera, C. R. (2003) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(02)00223-5 · Cited by 481
Microfluidics for flow cytometric analysis of cells and particles
Huh, D., Gu, W., Kamotani, Y., Grotberg, J. B., & Takayama, S. (2005) · Physiological Measurement · doi:10.1088/0967-3334/26/3/r02 · Cited by 396
Microfluidic approaches for cancer cell detection, characterization, and separation
Chen, J., Li, J., & Sun, Y. (2012) · Lab on a Chip · doi:10.1039/c2lc21273k · Cited by 301
Metadata
- Authors
- Yang, J., Huang, Y., Wang, X.-B., Becker, F. F., & Gascoyne, P. R. C.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-01-28
- Volume
- 71
- Issue
- 5
- Pages
- 911-918
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2087096498
- Cited by
- 310
- References
- 17
- FWCI
- 8.76
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/Gravitational Field-Flow Fractionation. https://app.cochat.ai/papers/10.1021/ac981250p
- The paper
- Yang, J., Huang, Y., Wang, X.-B., Becker, F. F., & Gascoyne, P. R. C. (1999). Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/Gravitational Field-Flow Fractionation. Analytical Chemistry. https://doi.org/10.1021/ac981250p
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
