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Amino Acid Analysis by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
Abstract
A method for the determination of underivatized amino acids based on capillary electrophoresis coupled to electrospray ionization mass spectrometry (CE-ESI-MS) is described. To analyze free amino acids simultaneously a low acidic pH condition was used to confer positive charge on whole amino acids. The choice of the electrolyte and its concentration influenced resolution and peak shape of the amino acids, and 1 M formic acid was selected as the optimal electrolyte. Meanwhile, the sheath liquid composition had a significant effect on sensitivity and the highest sensitivity was obtained when 5 mM ammonium acetate in 50% (v/v) methanol-water was used. Protonated amino acids were roughly separated by CE and selectively detected by a quadrupole mass spectrometer with a sheath flow electrospray ionization interface. Under the optimized conditions, 19 free amino acids normally found in proteins and several physiological amino acids were well determined in less than 17 min. The detection limits for basic amino acids were between 0.3 and 1.1 mumol/L and for acidic and low molecular weight amino acids were less than 6.0 mumol/L with pressure injection of 50 mbar for 3 s (3 nL) at a signal-to-noise ratio of 3. This method is simple, rapid, and selective compared with conventional techniques and could be readily applied to the analysis of free amino acids in soy sauce.
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Cited by
Hirayama, A., Kami, K., Sugimoto, M., Sugawara, M., Toki, N., Onozuka, H., Kinoshita, T., Saito, N., Ochiai, A., Tomita, M., et al. (2009) · Cancer Research · doi:10.1158/0008-5472.can-08-4806 · Cited by 956
Self-citationSoga, T., Ueno, Y., Naraoka, H., Ohashi, Y., Tomita, M., & Nishioka, T. (2002) · Analytical Chemistry · doi:10.1021/ac020064n · Cited by 472
Self-citationMetabolome analysis by capillary electrophoresis–mass spectrometry
Monton, M. R. N., & Soga, T. (2007) · Journal of Chromatography A · doi:10.1016/j.chroma.2007.02.065 · Cited by 318
Self-citationCapillary electrophoresis – mass spectrometry: 15 years of developments and applications
Schmitt‐Kopplin, P., & Frommberger, M. (2003) · ELECTROPHORESIS · doi:10.1002/elps.200305659 · Cited by 289
Sato, S., Soga, T., Nishioka, T., & Tomita, M. (2004) · The Plant Journal · doi:10.1111/j.1365-313x.2004.02187.x · Cited by 259
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“The analytical conditions for cationic and anionic metabolite analyses were as described previously (Soga and Heiger, 2000; Hasunuma et al., 2011), with minor modifications as described below.”
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Metadata
- Authors
- Soga, T., & Heiger, D. N.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-02-18
- Volume
- 72
- Issue
- 6
- Pages
- 1236-1241
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1995888001
- Cited by
- 519
- References
- 0
- FWCI
- 12.13
- Type
- journal article
- Language
- en
- Open access
- closed
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