Reaction-Activated Disassembly of the NIR-II Probe Enables Fast Detection and Ratiometric Photoacoustic Imaging of Glutathione In Vivo

Zhao, Yixing; Li, Lingyun; Ye, Qiaozhen; Gong, Yijun; Yang, Ronghua; Liu, Hongwen

doi:10.1021/acs.analchem.3c02664

Cited by 47Sulfur Compounds in BiologyMolecular Sensors and Ion DetectionNanoplatforms for cancer theranostics

Reaction-Activated Disassembly of the NIR-II Probe Enables Fast Detection and Ratiometric Photoacoustic Imaging of Glutathione In Vivo

Zhao, Yixing, Li, Lingyun, Ye, Qiaozhen, Gong, Yijun, Yang, Ronghua, Liu, Hongwen·2023·Analytical Chemistry

DOI 10.1021/acs.analchem.3c02664

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Abstract

Glutathione (GSH), the most abundant nonprotein biothiol, is a significant endogenous molecule that plays a key role in redox equilibrium in vivo and is regarded as a critical biomarker of cancer. Currently, various fluorescent probes have been designed and synthesized for imaging GSH at the cellular level in the visible range and the first near-infrared window (NIR-I, 750–900 nm). However, the application of these fluorescent probes for bioimaging and biosensing in vivo has been extremely hindered by the high biobackground and low tissue penetration. Herein, based on the self-assembly and disassembly of J-aggregation, we designed and synthesized a GSH-activatable probe MC-PSE for second near-infrared window (NIR-II) fluorescence and ratiometric photoacoustic imaging of GSH in vivo. The anionic cyanine-based MC-PSE tends to form stable J-aggregates in an aqueous solution. Upon the reaction with GSH, the J-aggregates of MC-PSE disassembled, the emission peak intensity of MC-PSE at 940 nm significantly increased by about 20 times, and the PA 900 /PA 980 ratio increased by 4 times within 15 min in vitro. Notably, we used MC-PSE to visualize GSH in tumor-bearing mice and to distinguish normal and tumor areas successfully by virtue of NIR-II FL and PA dual-modal imaging. The design strategy of MC-PSE provides a novel method for ratiometric photoacoustic imaging, and MC-PSE is expected to be a powerful tool for the accurate detection of GSH in cancer diagnosis.

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Liu et al. developed a GSH-responsive NIR-II fluorescence/PA dual-mode probe (MC-PSE).
“Subsequently, Liu et al. developed a GSH-responsive NIR-II fluorescence/PA dual-mode probe (MC-PSE) (Fig. 19b)221.”
From Organic small-molecule NIR-II fluorophores for tumor phototheranostics (2026) · cites this paper as [221]
MC-PSE tended to form stable J-aggregates in aqueous solution, and the presence of GSH triggered the disassembly of these J-aggregates, leading to the enhancement of fluorescent signals at 940 nm and the attenuation of PA signals at 980 nm.
“It tended to form stable J-aggregates in aqueous solution, and the presence of GSH triggered the disassembly of these J-aggregates, leading to the enhancement of fluorescent signals at 940 nm and the attenuation of PA signals at 980 nm.”
From Organic small-molecule NIR-II fluorophores for tumor phototheranostics (2026) · cites this paper as [221]

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Authors: Zhao, Y., Li, L., Ye, Q., Gong, Y., Yang, R., & Liu, H.
Affiliations: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410082, P. R. China
Hunan Normal University (CN)
Henan Normal University (CN)
Corresponding author: Gong, Yijun
ORCID: Li, Lingyun
Gong, Yijun
Yang, Ronghua
Liu, Hongwen
Venue: Analytical Chemistry
Publisher: American Chemical Society (ACS)
Published: 2023-09-07
Volume: 95
Issue: 37
Pages: 14043-14051
ISSN: 0003-2700 (ISSN-L)
1520-6882
DOI: 10.1021/acs.analchem.3c02664
OpenAlex: W4386509903
Cited by: 47
References: 46
Citations by year: 2026: 15 · 2025: 19 · 2024: 13
FWCI: 4.71
Type: journal article
Language: en
Open access: closed
Licence: doi.org/10.15223/policy-029 (stm-asf)
doi.org/10.15223/policy-037 (stm-asf)
doi.org/10.15223/policy-045 (stm-asf)
Locations: Analytical Chemistry (publishedVersion)
PubMed (publishedVersion)
Funders: State Key Laboratory of Chemo/Biosensing and Chemometrics (202201)
Scientific Research Foundation of Hunan Provincial Education Department (22A0027)
National Natural Science Foundation of China (21735001, 22104036)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
Keywords: glutathione detection, NIR-II fluorescence, cyanine dyes, self-assembly, dual-modality imaging, tumor imaging, tumor markers, biothiols
MeSH terms: Animals, Diagnostic Imaging, Fluorescent Dyes, Glutathione, Quinolines, Mice, Photoacoustic Techniques

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Reaction-Activated Disassembly of the NIR-II Probe Enables Fast Detection and Ratiometric Photoacoustic Imaging of Glutathione In Vivo

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