Cited by 45Sulfur Compounds in BiologyMolecular Sensors and Ion DetectionLuminescence and Fluorescent Materials

Activatable NIR-II Fluorescence Probe for Highly Sensitive and Selective Visualization of Glutathione In Vivo

Pan, Yuantao, Lei, Shan, Zhang, Jing, Qu, Junle, Huang, Peng, Lin, Jing·2021·Analytical Chemistry

Abstract

Visualization of glutathione (GSH) enables us to understand GSH-related pathophysiological processes in living subjects. Currently, in vivo visualization methods of GSH are based on visible or first near-infrared (NIR-I) window fluorescence (FL) probes, which possess limitations due to their low tissue penetration depth and strong tissue autofluorescence. Herein, we developed a GSH-activatable second near-infrared (NIR-II) window FL probe (denoted as LET-7) for highly sensitive and selective visualization of GSH in vivo . LET-7, composed of an anionic polymethylcyanide skeleton linked with a FL quenching group of 3,5-bis(trifluoromethyl)benzenethiol, can be specifically activated by GSH, thus triggering a significant NIR-II FL emission enhancement with excellent photostability, which enables us to efficiently distinguish GSH from closely related low-molecular-weight biothiols. The limit of detection of LET-7 for GSH was determined to be as low as 85 nM. Most intriguingly, the in vivo studies demonstrated that LET-7 showed high sensitivity and good selectivity toward GSH. Therefore, our study provides a solution to design activatable NIR-II FL probes for in vivo imaging of GSH and other disease-related biomarkers.

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Crossref · 10.1021/acs.analchem.1c04504

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  • Lin et al. pioneered the development of a GSH-activatable NIR-II probe (LET-7), enabling highly sensitive and selective visualization of GSH in vivo.

    “Lin et al. pioneered the development of a GSH-activatable NIR-II probe (LET-7), enabling highly sensitive and selective visualization of GSH in vivo (Fig. 19a)220.”
  • Upon exposure to GSH, LET-7 exhibited a significant fluorescence enhancement at 928 nm, achieving a remarkably low detection limit of 85 nM.

    “Upon exposure to GSH, LET-7 exhibited a significant fluorescence enhancement at 928 nm, achieving a remarkably low detection limit of 85 nM.”

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Authors
Pan, Y., Lei, S., Zhang, J., Qu, J., Huang, P., & Lin, J.
Affiliations
Shenzhen University (CN)
Corresponding author
Lin, Jing
Venue
Analytical Chemistry
Published
2021-12-14
Volume
93
Issue
51
Pages
17103-17109
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
OpenAlex
W4200202181
Cited by
45
References
35
Citations by year
2026: 4 · 2025: 16 · 2024: 8 · 2023: 11 · 2022: 6
FWCI
2.58
Type
journal article
Language
en
Open access
closed
Locations
  • Analytical Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Science and Technology of the People's Republic of China (2018YFA0704000, 2020YFA0908800)
  • Basic Research Program of Shenzhen (JCYJ20180507182413022, JCYJ20200109105620482)
  • Shenzhen Science and Technology Program (KQTD20190929172538530)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
  • Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
  • Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
Keywords
glutathione, activatable fluorescent probes, in vivo imaging, fluorescence quenching, photostability, biothiol selectivity, let-7 microRNA
MeSH terms
Diagnostic Imaging, Fluorescence, Fluorescent Dyes, Glutathione, Humans

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