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A Screen for Membrane Fission Catalysts Identifies the ATPase EHD1

Kamerkar, Sukrut C., Roy, Krishnendu, Bhattacharyya, Soumya, Pucadyil, Thomas J.·2019·Biochemistry
DOI 10.1021/acs.biochem.8b00925PMC PMC6327249
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Abstract

Membrane fission manifests during cell division, synaptic transmission, vesicular transport, and organelle biogenesis, yet identifying proteins that catalyze fission remains a challenge. Using a facile and robust assay system of supported membrane tubes in a microscopic screen that directly monitors membrane tube scission, we detect robust GTP- and ATP-dependent as well as nucleotide-independent fission activity in the brain cytosol. Using previously established interacting partner proteins as bait for pulldowns, we attribute the GTP-dependent fission activity to dynamin. Biochemical fractionation followed by mass spectrometric analyses identifies the Eps15-homology domain-containing protein1 (EHD1) as a novel ATP-dependent membrane fission catalyst. Together, our approach establishes an experimental workflow for the discovery of novel membrane fission catalysts.

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Metadata

Authors
Kamerkar, S. C., Roy, K., Bhattacharyya, S., & Pucadyil, T. J.
Affiliations
Indian Institute of Science Education and Research , Dr. Homi Bhabha Road , Pashan, , ,
ORCID
Pucadyil, Thomas J.
Venue
Biochemistry
Publisher
American Chemical Society (ACS)
Published
2018-11-07
Volume
58
Issue
1
Pages
65-71
ISSN
  • 0006-2960
  • 1520-4995
DOI
10.1021/acs.biochem.8b00925
PMC
PMC6327249
Cited by
18
References
1
Language
en
Licence
pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html (vor, 9 d)
Funders
  • Howard Hughes Medical Institute (NA)
  • Wellcome Trust (IA/S/16/2/502708)
  • Department of Biotechnology , Ministry of Science and Technology (IA/S/16/2/502708)
  • Council of Scientific and Industrial Research (NA)
  • Indian Institute of Science Education and Research Pune (NA)

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