The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Isothermal Amplification of Nucleic Acids
Abstract
Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently accumulates nucleic acid sequences at constant temperature. Since the early 1990s, various isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR). These isothermal amplification methods have been used for biosensing targets such as DNA, RNA, cells, proteins, small molecules, and ions. The applications of these techniques for in situ or intracellular bioimaging and sequencing have been amply demonstrated. Amplicons produced by isothermal amplification methods have also been utilized to construct versatile nucleic acid nanomaterials for promising applications in biomedicine, bioimaging, and biosensing. The integration of isothermal amplification into microsystems or portable devices improves nucleic acid-based on-site assays and confers high sensitivity. Single-cell and single-molecule analyses have also been implemented based on integrated microfluidic systems. In this review, we provide a comprehensive overview of the isothermal amplification of nucleic acids encompassing work published in the past two decades. First, different isothermal amplification techniques are classified into three types based on reaction kinetics. Then, we summarize the applications of isothermal amplification in bioanalysis, diagnostics, nanotechnology, materials science, and device integration. Finally, several challenges and perspectives in the field are discussed.
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Cites
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Genome-Wide Detection of Single-Nucleotide and Copy-Number Variations of a Single Human Cell
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Device integration of electrochemical biosensors
Wu, J., Liu, H., Chen, W., Ma, B., & Ju, H. (2023) · Nature Reviews Bioengineering · doi:10.1038/s44222-023-00032-w · Cited by 895
Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care Diagnosis
Liu, Y., Zhan, L., Qin, Z., Sackrison, J., & Bischof, J. C. (2021) · ACS Nano · doi:10.1021/acsnano.0c10035 · Cited by 626
Point-of-care diagnostics for infectious diseases: From methods to devices
Wang, C., Liu, M., Wang, Z., Li, S., Deng, Y., & He, N. (2021) · Nano Today · doi:10.1016/j.nantod.2021.101092 · Cited by 622
DNA Functional Materials Assembled from Branched DNA: Design, Synthesis, and Applications
Dong, Y., Yao, C., Zhu, Y., Yang, L., Luo, D., & Yang, D. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00294 · Cited by 533
Metadata
- Authors
- Zhao, Y., Chen, F., Li, Q., Wang, L., & Fan, C.
- Venue
- Chemical Reviews
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-11-09
- Volume
- 115
- Issue
- 22
- Pages
- 12491-12545
- ISSN
- 0009-2665 (ISSN-L)
- 1520-6890
- OpenAlex
- W2145303234
- Cited by
- 1,775
- References
- 9
- FWCI
- 7.22
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zhao, Y., Chen, F., Li, Q., Wang, L., & Fan, C. (2015). Isothermal Amplification of Nucleic Acids. Chemical Reviews. https://doi.org/10.1021/acs.chemrev.5b00428
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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