Cited by 120Spectroscopy and Quantum Chemical StudiesPhotosynthetic Processes and MechanismsPhotochemistry and Electron Transfer Studies

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Single-Molecule Fluorescence Spectroscopy of Photosynthetic Systems

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Kondo, Toru, Chen, Wei Jia, Schlau-Cohen, Gabriela S.·2017·Chemical Reviews

DOI 10.1021/acs.chemrev.6b00195

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Abstract

Photosynthesis begins when a network of pigment-protein complexes captures solar energy and transports it to the reaction center, where charge separation occurs. When necessary (under low light conditions), photosynthetic organisms perform this energy transport and charge separation with near unity quantum efficiency. Remarkably, this high efficiency is maintained under physiological conditions, which include thermal fluctuations of the pigment-protein complexes and changing local environments. These conditions introduce multiple types of heterogeneity in the pigment-protein complexes, including structural heterogeneity, energetic heterogeneity, and functional heterogeneity. Understanding how photosynthetic light-harvesting functions in the face of these fluctuations requires understanding this heterogeneity, which, in turn, requires characterization of individual pigment-protein complexes. Single-molecule spectroscopy has the power to probe individual complexes. In this review, we present an overview of the common techniques for single-molecule fluorescence spectroscopy applied to photosynthetic systems and describe selected experiments on these systems. We discuss how these experiments provide a new understanding of the impact of heterogeneity on light harvesting and thus how these systems are optimized to capture sunlight under physiological conditions.

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Authors: Kondo, T., Chen, W. J., & Schlau-Cohen, G. S.
Affiliations: Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge Massachusetts 02139, United States
Massachusetts Institute of Technology (US)
ORCID: Chen, Wei Jia
Schlau-Cohen, Gabriela S.
Venue: Chemical Reviews
Publisher: American Chemical Society (ACS)
Published: 2017-01-10
Volume: 117
Issue: 2
Pages: 860-898
ISSN: 0009-2665 (ISSN-L)
1520-6890
DOI: 10.1021/acs.chemrev.6b00195
OpenAlex: W2568620643
Cited by: 120
References: 320
Citations by year: 2026: 6 · 2025: 13 · 2024: 16 · 2023: 10 · 2022: 16 · 2021: 11 · 2020: 13 · 2019: 10 · 2018: 16 · 2017: 9
FWCI: 0.87
Type: journal article
Language: en
Open access: green
www.osti.gov
Locations: Chemical Reviews (publishedVersion)
PubMed (publishedVersion)
OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
Funders: Richard and Susan Smith Family Foundation (Excellence in Biomedical Research Award)
Basic Energy Sciences (Center for Excitonics/DE-SC0001088)
Topics: Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Photochemistry and Electron Transfer Studies
Keywords: photosynthesis, light-harvesting complexes, single-molecule fluorescence spectroscopy, pigment-protein complexes, structural heterogeneity, energetic heterogeneity, functional heterogeneity, thermal fluctuations, energy transport, charge separation, reaction center, quantum efficiency, low-light adaptation
MeSH terms: Photosynthesis, Pigments, Biological, Proteins, Spectrometry, Fluorescence

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