Ultrafast energy relaxation in single light-harvesting complexes
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Significance: The excitation energy transfer in light-harvesting complexes is usually studied either by ultrafast bulk spectroscopy or by single-molecule spectroscopy. These methods are to a high degree complementary: Bulk spectroscopy measures ultrafast processes averaged over thousands of complexes, whereas single-molecule spectroscopy observes much slower dynamics in individual complexes. In this work, we combine these approaches using a recently developed ultrafast single-molecule spectroscopy technique. This enables us, for the first time (to our knowledge), to observe ultrafast energy relaxation with a rate around 100 fs in individual light-harvesting complexes. We determine the distribution of the relaxation times, observe changes of the relaxation time in one complex, and find how the relaxation depends on excitation wavelength.
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Developments in experimental techniques such as ultrafast spectroscopy, single molecule spectroscopy, time-resolved microscopy and single particle imaging have enabled study of biological dynamics on increasingly small length and time scales, revealing processes necessary for living function that depend on a delicate interplay between quantum and classical physical effects.
“Recently, developments in experimental techniques such as ultrafast spectroscopy [6], single molecule spectroscopy [7 -11], timeresolved microscopy [12 -14] and single particle imaging [15 -18] have enabled us to study biological dynamics on increasingly small length and time scales, revealing a variety of processes necessary for the function of the living system that depend on a delicate interpl”
From The future of quantum biology (2018) · cites this paper as [11]
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- Authors
- Malý, P., Gruber, J. M., Cogdell, R. J., Mančal, T., & van Grondelle, R.
- Affiliations
- Charles University (CZ)
- Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit Amsterdam, 1081HV Amsterdam, The Netherlands;
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2016-02-22
- Volume
- 113
- Issue
- 11
- Pages
- 2934-2939
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4801264
- OpenAlex
- W2280431368
- Cited by
- 46
- References
- 34
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 5 · 2023: 7 · 2022: 5 · 2021: 6 · 2020: 4 · 2019: 2 · 2018: 6 · 2017: 5 · 2016: 1
- FWCI
- 2.21
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- VU Research Portal (publishedVersion, other-oa)
- arXiv (Cornell University) (submittedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- arXiv (Cornell University)
- Funders
- EC | European Research Council (267333)
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (700.58.305)
- Seventh Framework Programme (GA 323901)
- Czech Science Foundation (14-25752S)
- U.S. Department of Energy (DE-SC0001035)
- Topics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
- Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Photochemistry and Electron Transfer Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- light-harvesting complexes, LH2, purple bacteria, single-molecule fluorescence spectroscopy, pump-probe spectroscopy, distribution of relaxation times, Redfield theory, Gaussian disorder, ergodicity, excited states
- MeSH terms
- Bacteriochlorophylls, Energy Transfer, Lasers, Light, Rhodopseudomonas, Spectrometry, Fluorescence, Time, Normal Distribution, Microscopy, Confocal, Statistics, Nonparametric, Light-Harvesting Protein Complexes
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