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Stimulated Emission Depletion Microscopy

Blom, Hans, Widengren, Jerker·2017·Chemical Reviews

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Abstract

Despite its short history, diffraction-unlimited fluorescence microscopy techniques have already made a substantial imprint in the biological sciences. In this review, we describe how stimulated emission depletion (STED) imaging originally evolved, how it compares to other optical super-resolution imaging techniques, and what advantages it provides compared to previous golden-standards for biological microscopy, such as diffraction-limited optical microscopy and electron microscopy. We outline the prerequisites for successful STED imaging experiments, emphasizing the equally critical roles of instrumentation, sample preparation, and photophysics, and describe major evolving strategies for how to push the borders of STED imaging even further in life science. Finally, we provide examples of how STED nanoscopy can be applied, within three different fields with particular potential for STED imaging experiments: neuroscience, plasma membrane biophysics, and subcellular clinical diagnostics. In these areas, and in many more, STED imaging can be expected to play an increasingly important role in the future.

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Oncogenes induce a vimentin filament collapse mediated by HDAC6 that is linked to cell stiffness
Rathje, L.-S. Z., Nordgren, N., Pettersson, T., Rönnlund, D., Widengren, J., Aspenström, P., & Gad, A. K. B. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1300238111 · Cited by 121
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The development of the electron microscope and of electron microscopy
Ruska, E. (1987) · Bioscience Reports · doi:10.1007/bf01127674 · Cited by 120
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Self-citation
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Hinterdorfer, P., Parajo, M. F. G., & Dufrêne, Y. F. (2011) · Accounts of Chemical Research · doi:10.1021/ar2001167 · Cited by 92
Three-dimensional super-resolution with a 4Pi-confocal microscope using image restoration
Schrader, M., Hell, S. W., & van der Voort, H. T. M. (1998) · Journal of Applied Physics · doi:10.1063/1.368616 · Cited by 92
The theory of the direct‐view confocal microscope
Sheppard, C. J. R., & Wilson, T. (1981) · Journal of Microscopy · doi:10.1111/j.1365-2818.1981.tb00304.x · Cited by 92
3D reconstruction of high‐resolution STED microscope images
Punge, A., Rizzoli, S. O., Jahn, R., Wildanger, D., Meyer, L., Schönle, A., Kastrup, L., & Hell, S. W. (2008) · Microscopy Research and Technique · doi:10.1002/jemt.20602 · Cited by 91
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Becker, W., Su, B., Holub, O., et al. (2010) · Microscopy Research and Technique · doi:10.1002/jemt.20959 · Cited by 90
Light exposure and cell viability in fluorescence microscopy
Schneckenburger, H., Weber, P., Wagner, M., Schickinger, S., Richter, V., Bruns, T., Strauß, W. S. L., & Wittig, R. (2011) · Journal of Microscopy · doi:10.1111/j.1365-2818.2011.03576.x · Cited by 90
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Ringemann, C., Harke, B., von Middendorff, C., Medda, R., Honigmann, A., Wagner, R., Leutenegger, M., Schönle, A., Hell, S. W., & Eggeling, C. (2009) · New Journal of Physics · doi:10.1088/1367-2630/11/10/103054 · Cited by 88
Progress in quantitative single-molecule localization microscopy
Deschout, H. G., Shivanandan, A., Annibale, P., Scarselli, M., & Rađenović, A. (2014) · Histochemistry and Cell Biology · doi:10.1007/s00418-014-1217-y · Cited by 88
Dual color localization microscopy of cellular nanostructures
Gunkel, M., Erdel, F., Rippe, K., Lemmer, P., Kaufmann, R., Hörmann, C., Amberger, R., & Cremer, C. G. (2009) · Biotechnology Journal · doi:10.1002/biot.200900005 · Cited by 86
Challenges in quantitative single molecule localization microscopy
Shivanandan, A., Deschout, H. G., Scarselli, M., & Rađenović, A. (2014) · FEBS Letters · doi:10.1016/j.febslet.2014.06.014 · Cited by 86
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Hansson, M., Ringdahl, J., Robert, A., Power, U. F., Goetsch, L., Nguyen, T. N., Uhlén, M., Ståhl, S., & Nygren, P. (1999) · Immunotechnology · doi:10.1016/s1380-2933(98)00026-8 · Cited by 85
STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins
Fitzpatrick, J. A. J., Yan, Q., Sieber, J. J., Dyba, M., Schwarz, U., Szent-Györgyi, C., Woolford, C. A., Berget, P. B., Waggoner, A. S., & Bruchez, M. P. (2009) · Bioconjugate Chemistry · doi:10.1021/bc900249e · Cited by 85
Probing cytoskeletal structures by coupling optical superresolution and AFM techniques for a correlative approach
Chacko, J. V., Zanacchi, F. C., & Diaspro, A. (2013) · Cytoskeleton · doi:10.1002/cm.21139 · Cited by 84
Super-resolution imaging in live cells
Cox, S. (2014) · Developmental Biology · doi:10.1016/j.ydbio.2014.11.025 · Cited by 84
Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT
Hoyer, P., de Medeiros, G., Balázs, B., Norlin, N., Besir, C., Hanne, J., Kräusslich, H., Engelhardt, J., Sahl, S. J., Hell, S. W., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1522292113 · Cited by 84
Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability
Bossi, M. L., Fölling, J., Dyba, M., Westphal, V., & Hell, S. W. (2006) · New Journal of Physics · doi:10.1088/1367-2630/8/11/275 · Cited by 83
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Autofluorescence generation and elimination: a lesson from glutaraldehyde
Lee, K., Choi, S., Yang, C. Z., Wu, H., & Yu, J. (2013) · Chemical Communications · doi:10.1039/c3cc40799c · Cited by 79
Correlative Photoactivated Localization and Scanning Electron Microscopy
Kopek, B. G., Shtengel, G. E., Grimm, J. B., Clayton, D. A., & Hess, H. F. (2013) · PLoS ONE · doi:10.1371/journal.pone.0077209 · Cited by 74
Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS)
Leutenegger, M., Ringemann, C., Lasser, T., Hell, S. W., & Eggeling, C. (2012) · Optics Express · doi:10.1364/oe.20.005243 · Cited by 73
Multiparameter fluorescence imagespectroscopy to study molecular interactions
Weidtkamp‐Peters, S., Felekyan, S., Bleckmann, A., Simon, R. G., Becker, W. H., Kühnemuth, R., & Seidel, C. A. M. (2009) · Photochemical & Photobiological Sciences · doi:10.1039/b903245m · Cited by 71
Laser-diode-stimulated emission depletion microscopy
Westphal, V., Blanca, C. M., Dyba, M., Kastrup, L., & Hell, S. W. (2003) · Applied Physics Letters · doi:10.1063/1.1571656 · Cited by 71
Dual-Label STED Nanoscopy of Living Cells Using Photochromism
Willig, K. I., Stiel, A. C., Brakemann, T., Jakobs, S., & Hell, S. W. (2011) · Nano Letters · doi:10.1021/nl202290w · Cited by 68
Mechanism of Intramolecular Photostabilization in Self‐Healing Cyanine Fluorophores
van der Velde, J. H. M., Ploetz, E., Hiermaier, M., Oelerich, J., de Vries, J. W., Roelfes, G., & Cordes, T. (2013) · ChemPhysChem · doi:10.1002/cphc.201300785 · Cited by 68
Light Dose is a Limiting Factor to Maintain Cell Viability in Fluorescence Microscopy and Single Molecule Detection
Wagner, M., Weber, P., Bruns, T., Strauß, W. S. L., Wittig, R., & Schneckenburger, H. (2010) · International Journal of Molecular Sciences · doi:10.3390/ijms11030956 · Cited by 67
LOTOS-based two-photon calcium imaging of dendritic spines in vivo
Chen, X., Leischner, U., Varga, Z., Jia, H., Deca, D., Rochefort, N. L., & Konnerth, A. (2012) · Nature Protocols · doi:10.1038/nprot.2012.106 · Cited by 66
Delayed emergence of subdiffraction-sized mutant huntingtin fibrils following inclusion body formation
Sahl, S. J., Lau, L., Vonk, W. I. M., Weiss, L. E., Frydman, J., & Moerner, W. E. (2015) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583515000219 · Cited by 65
Finding, defining and breaking the diffraction barrier in microscopy – a historical perspective
Lauterbach, M. A. (2012) · Optical Nanoscopy · doi:10.1186/2192-2853-1-8 · Cited by 64
Triplet-relaxation microscopy with bunched pulsed excitation
Donnert, G., Eggeling, C., & Hell, S. W. (2009) · Photochemical & Photobiological Sciences · doi:10.1039/b903357m · Cited by 62
The Probe Rules in Single Particle Tracking
Clausen, M. P., & Lagerholm, B. C. (2011) · Current Protein and Peptide Science · doi:10.2174/138920311798841672 · Cited by 60
Highly Sensitive FRET-FCS Detects Amyloid β-Peptide Oligomers in Solution at Physiological Concentrations
Wennmalm, S., Chmyrov, V., Widengren, J., & Tjernberg, L. O. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b02630 · Cited by 60
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Tian, X. Y., Pahlavan, S., Oleinikow, K., Jung, J., Ruppenthal, S., Scholz, A., Schumann, C., Kraegeloh, A., Oberhofer, M., Lipp, P., et al. (2011) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2011.09.001 · Cited by 57
4Pi-RESOLFT nanoscopy
Boehm, U., Hell, S. W., & Schmidt, R. (2016) · Nature Communications · doi:10.1038/ncomms10504 · Cited by 57
Intra-molecular triplet energy transfer is a general approach to improve organic fluorophore photostability
Zheng, Q., Jockusch, S., Rodríguez-Calero, G. G., Zhou, Z., Zhao, H. C., Altman, R. B., Abruña, H. D., & Blanchard, S. C. (2015) · Photochemical & Photobiological Sciences · doi:10.1039/c5pp00400d · Cited by 56
STED microscopy for nanoscale imaging in living brain slices
Chéreau, R., Tønnesen, J., & Nägerl, U. V. (2015) · Methods · doi:10.1016/j.ymeth.2015.06.006 · Cited by 53
isoSTED nanoscopy with intrinsic beam alignment
Curdt, F., Herr, S. J., Lutz, T., Schmidt, R., Engelhardt, J., Sahl, S. J., & Hell, S. W. (2015) · Optics Express · doi:10.1364/oe.23.030891 · Cited by 50
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Ilgen, P., Stoldt, S., Conradi, L., Wurm, C. A., Rüschoff, J., Ghadimi, Β. M., Liersch, T., & Jakobs, S. (2014) · PLoS ONE · doi:10.1371/journal.pone.0101563 · Cited by 49
Diffraction-unlimited imaging: from pretty pictures to hard numbers
Vandenberg, W., Leutenegger, M., Lasser, T., Hofkens, J., & Dedecker, P. (2015) · Cell and Tissue Research · doi:10.1007/s00441-014-2109-0 · Cited by 49
STED microscopy: increased resolution for medical research?
Blom, H., & Brismar, H. (2014) · Journal of Internal Medicine · doi:10.1111/joim.12278 · Cited by 48
Self-citation
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Schröder, J., Benink, H. A., Dyba, M., & Los, G. V. (2009) · Biophysical Journal · doi:10.1016/j.bpj.2008.09.032 · Cited by 48
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See all 339

Authors: Blom, H., & Widengren, J.
Venue: Chemical Reviews
Publisher: American Chemical Society (ACS)
Published: 2017-03-06
Volume: 117
Issue: 11
Pages: 7377-7427
ISSN: 0009-2665 (ISSN-L)
1520-6890
DOI: 10.1021/acs.chemrev.6b00653
OpenAlex: W2594822719
Cited by: 339
References: 320
FWCI: 2.30
Type: journal article
Language: en
Open access: closed

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Stimulated Emission Depletion Microscopy

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