The DOI matches its Crossref record; 320 of the 320 references it cites were found in a registry or an index.
Stimulated Emission Depletion Microscopy
Abstract
Despite its short history, diffraction-unlimited fluorescence microscopy techniques have already made a substantial imprint in the biological sciences. In this review, we describe how stimulated emission depletion (STED) imaging originally evolved, how it compares to other optical super-resolution imaging techniques, and what advantages it provides compared to previous golden-standards for biological microscopy, such as diffraction-limited optical microscopy and electron microscopy. We outline the prerequisites for successful STED imaging experiments, emphasizing the equally critical roles of instrumentation, sample preparation, and photophysics, and describe major evolving strategies for how to push the borders of STED imaging even further in life science. Finally, we provide examples of how STED nanoscopy can be applied, within three different fields with particular potential for STED imaging experiments: neuroscience, plasma membrane biophysics, and subcellular clinical diagnostics. In these areas, and in many more, STED imaging can be expected to play an increasingly important role in the future.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
Binnig, G., Quate, C. F., & Gerber, C. (1986) · Physical Review Letters · doi:10.1103/physrevlett.56.930 · Cited by 14,762
Two-photon laser scanning fluorescence microscopy
Denk, W., Strickler, J. H., & Webb, W. W. (1990) · Science · doi:10.1126/science.2321027 · Cited by 9,488
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
Betzig, E., Patterson, G. H., Sougrat, R., Lindwasser, O. W., Olenych, S., Bonifacino, J. S., Davidson, M. W., Lippincott-Schwartz, J., & Hess, H. F. (2006) · Science · doi:10.1126/science.1127344 · Cited by 8,772
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
Rust, M. J., Bates, M., & Zhuang, X. (2006) · Nature Methods · doi:10.1038/nmeth929 · Cited by 8,078
Green fluorescent protein as a marker for gene expression
Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., & Prasher, D. C. (1994) · Science · doi:10.1126/science.8303295 · Cited by 6,802
Communication in the presence of noise
Shannon, C. E. (1949) · Proceedings of the IRE · doi:10.1109/jrproc.1949.232969 · Cited by 6,539
Hell, S. W., & Wichmann, J. (1994) · Optics Letters · doi:10.1364/ol.19.000780 · Cited by 6,065
Lipid rafts as a membrane-organizing principle
Lingwood, D., & Simons, K. (2010) · Science · doi:10.1126/science.1174621 · Cited by 4,417
Nonlinear magic: multiphoton microscopy in the biosciences
Zipfel, W. R., Williams, R. M., & Webb, W. W. (2003) · Nature Biotechnology · doi:10.1038/nbt899 · Cited by 3,893
Gustafsson, M. G. L. (2000) · Journal of Microscopy · doi:10.1046/j.1365-2818.2000.00710.x · Cited by 3,786
Handbook Of Biological Confocal Microscopy
(2006) · Springer eBooks · doi:10.1007/978-0-387-45524-2 · Cited by 3,637
Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy
Hess, S. T., Girirajan, T. P. K., & Mason, M. D. (2006) · Biophysical Journal · doi:10.1529/biophysj.106.091116 · Cited by 3,605
Certain Topics in Telegraph Transmission Theory
Nyquist, H. (1928) · Transactions of the American Institute of Electrical Engineers · doi:10.1109/t-aiee.1928.5055024 · Cited by 3,089
Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
The Fluorescent Toolbox for Assessing Protein Location and Function
Giepmans, B. N. G., Adams, S. R., Ellisman, M. H., & Tsien, R. Y. (2006) · Science · doi:10.1126/science.1124618 · Cited by 2,770
Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung
Abbe, E. (1873) · Archiv für Mikroskopische Anatomie · doi:10.1007/bf02956173 · Cited by 2,724
Time-Correlated Single Photon Counting
(1984) · Elsevier eBooks · doi:10.1016/b978-0-12-524140-3.x5001-1 · Cited by 2,550
Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy
Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J., & Stelzer, E. H. K. (2004) · Science · doi:10.1126/science.1100035 · Cited by 2,355
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Gustafsson, M. G. L. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0406877102 · Cited by 2,224
Primary structure of the Aequorea victoria green-fluorescent protein
Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G., & Cormier, M. J. (1992) · Gene · doi:10.1016/0378-1119(92)90691-h · Cited by 2,177
Optical stethoscopy: Image recording with resolution λ/20
Pohl, D. W., Denk, W., & Lanz, M. (1984) · Applied Physics Letters · doi:10.1063/1.94865 · Cited by 2,040
Nanoscale imaging magnetometry with diamond spins under ambient conditions
Balasubramanian, G., Chan, I. Y., Kolesov, R., Al-Hmoud, M., Tisler, J., Shin, C., Kim, C., Wojcik, A., Hemmer, P. R., Krueger, A., et al. (2008) · Nature · doi:10.1038/nature07278 · Cited by 1,999
Nanoscale magnetic sensing with an individual electronic spin in diamond
Maze, J. R., Stanwix, P. L., Hodges, J. S., Hong, S., Taylor, J. M., Cappellaro, P., Jiang, L., Dutt, M. V. G., Togan, E., Zibrov, A. S., et al. (2008) · Nature · doi:10.1038/nature07279 · Cited by 1,984
Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
Chen, B.-C., Legant, W. R., Wang, K., Shao, L., Milkie, D. E., Davidson, M. W., Janetopoulos, C., Wu, X. S., Hammer, J. A., Liu, Z., et al. (2014) · Science · doi:10.1126/science.1257998 · Cited by 1,919
Nanomechanical analysis of cells from cancer patients
Cross, S. E., Jin, Y.-S., Rao, J., & Gimzewski, J. K. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.388 · Cited by 1,908
Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission
Klar, T. A., Jakobs, S., Dyba, M., Egner, A., & Hell, S. W. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.15.8206 · Cited by 1,869
Subdiffraction‐Resolution Fluorescence Imaging with Conventional Fluorescent Probes
Heilemann, M., van de Linde, S., Schüttpelz, M., Kasper, R., Seefeldt, B., Mukherjee, A., Tinnefeld, P., & Sauer, M. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200802376 · Cited by 1,866
Handbook of Biological Confocal Microscopy
(1995) · Springer eBooks · doi:10.1007/978-1-4757-5348-6 · Cited by 1,848
Near-Field Optics: Microscopy, Spectroscopy, and Surface Modification Beyond the Diffraction Limit
Betzig, E., & Trautman, J. K. (1992) · Science · doi:10.1126/science.257.5067.189 · Cited by 1,808
Thermodynamic Fluctuations in a Reacting System—Measurement by Fluorescence Correlation Spectroscopy
Magde, D., Elson, E., & Webb, W. W. (1972) · Physical Review Letters · doi:10.1103/physrevlett.29.705 · Cited by 1,753
A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells
Patterson, G. H., & Lippincott-Schwartz, J. (2002) · Science · doi:10.1126/science.1074952 · Cited by 1,612
Nanoscale chemical analysis by tip-enhanced Raman spectroscopy
Stöckle, R. M., Suh, Y. D., Deckert, V., & Zenobi, R. (2000) · Chemical Physics Letters · doi:10.1016/s0009-2614(99)01451-7 · Cited by 1,606
Gustafsson, M. G. L., Shao, L., Carlton, P. M., Wang, C. J. R., Golubovskaya, I. N., Cande, W. Z., Agard, D. A., & Sedat, J. W. (2008) · Biophysical Journal · doi:10.1529/biophysj.107.120345 · Cited by 1,557
Hu, C.-D., Chinenov, Y., & Kerppola, T. K. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00496-3 · Cited by 1,549
Direct observation of the nanoscale dynamics of membrane lipids in a living cell
Eggeling, C., Ringemann, C., Medda, R., Schwarzmann, G., Sandhoff, K., Polyakova, S., Belov, V. N., Hein, B., von Middendorff, C., Schönle, A., et al. (2009) · Nature · doi:10.1038/nature07596 · Cited by 1,520
Chen, F., Tillberg, P. W., & Boyden, E. S. (2015) · Science · doi:10.1126/science.1260088 · Cited by 1,501
Heim, R., & Tsien, R. Y. (1996) · Current Biology · doi:10.1016/s0960-9822(02)00450-5 · Cited by 1,480
Zero-Mode Waveguides for Single-Molecule Analysis at High Concentrations
Levene, M. J., Korlach, J., Turner, S. W., Foquet, M., Craighead, H. G., & Webb, W. W. (2003) · Science · doi:10.1126/science.1079700 · Cited by 1,378
On/off blinking and switching behaviour of single molecules of green fluorescent protein
Dickson, R. M., Cubitt, A. B., Tsien, R. Y., & Moerner, W. E. (1997) · Nature · doi:10.1038/41048 · Cited by 1,342
Fluorescent probes for super-resolution imaging in living cells
Fernández-Suárez, M., & Ting, A. Y. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2531 · Cited by 1,306
Novel optical approach to atomic force microscopy
Meyer, G., & Amer, N. M. (1988) · Applied Physics Letters · doi:10.1063/1.100061 · Cited by 1,290
Optical detection and spectroscopy of single molecules in a solid
Moerner, W. E., & Kador, L. (1989) · Physical Review Letters · doi:10.1103/physrevlett.62.2535 · Cited by 1,271
Actin, Spectrin, and Associated Proteins Form a Periodic Cytoskeletal Structure in Axons
Xu, K., Zhong, G., & Zhuang, X. (2013) · Science · doi:10.1126/science.1232251 · Cited by 1,246
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis
Willig, K. I., Rizzoli, S. O., Westphal, V., Jahn, R., & Hell, S. W. (2006) · Nature · doi:10.1038/nature04592 · Cited by 1,225
High-density mapping of single-molecule trajectories with photoactivated localization microscopy
Manley, S., Gillette, J. M., Patterson, G. H., Shroff, H., Hess, H. F., Betzig, E., & Lippincott-Schwartz, J. (2008) · Nature Methods · doi:10.1038/nmeth.1176 · Cited by 1,199
Scanning electrochemical microscopy. Introduction and principles
Bard, A. J., Fan, F. R. F., Kwak, J., & Lev, O. (1989) · Analytical Chemistry · doi:10.1021/ac00177a011 · Cited by 1,151
Wide-field subdiffraction imaging by accumulated binding of diffusing probes
Sharonov, A., & Hochstrasser, R. M. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0609643104 · Cited by 1,151
Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI)
Dertinger, T., Colyer, R., Iyer, G., Weiss, S., & Enderlein, J. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0907866106 · Cited by 1,130
Schermelleh, L., Carlton, P. M., Haase, S., Shao, L., Winoto, L., Kner, P., Burke, B., Cardoso, M. C., Agard, D. A., Gustafsson, M. G. L., et al. (2008) · Science · doi:10.1126/science.1156947 · Cited by 1,123
Kusumi, A., Nakada, C., Ritchie, K., Murase, K., Suzuki, K., Murakoshi, H., Kasai, R. S., Kondo, J., & Fujiwara, T. (2005) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.34.040204.144637 · Cited by 1,122
The Physical Principles of the Quantum Theory
Compton, A. H., & Heisenberg, W. (1984) · Scientific Review Papers, Talks, and Books Wissenschaftliche Übersichtsartikel, Vorträge und Bücher · doi:10.1007/978-3-642-61742-3_10 · Cited by 1,103
Kusumi, A., Sako, Y., & Yamamoto, M. (1993) · Biophysical Journal · doi:10.1016/s0006-3495(93)81253-0 · Cited by 1,053
Single pentacene molecules detected by fluorescence excitation in a p-terphenyl crystal
Orrit, M., & Bernard, J. (1990) · Physical Review Letters · doi:10.1103/physrevlett.65.2716 · Cited by 1,042
Imaging Crystals, Polymers, and Processes in Water with the Atomic Force Microscope
Drake, B., Prater, C. B., Weisenhorn, A. L., Gould, S. A. C., Albrecht, T. R., Quate, C. F., Cannell, D. S., Hansma, H. G., & Hansma, P. K. (1989) · Science · doi:10.1126/science.2928794 · Cited by 1,033
Rigler, R., Mets, �., Widengren, J., & Kask, P. (1993) · European Biophysics Journal · doi:10.1007/bf00185777 · Cited by 1,002
Self-citationBinnig, G., & Rohrer, H. (1983) · Surface Science · doi:10.1016/0039-6028(83)90716-1 · Cited by 979
Super-resolution Aperture Scanning Microscope
ASH, E. A., & NICHOLLS, G. (1972) · Nature · doi:10.1038/237510a0 · Cited by 953
Single defect centres in diamond: A review
Jelezko, F., & Wrachtrup, J. (2006) · physica status solidi (a) · doi:10.1002/pssa.200671403 · Cited by 930
Multicolor and Electron Microscopic Imaging of Connexin Trafficking
Gaietta, G., Deerinck, T. J., Adams, S. R., Bouwer, J., Tour, O., Laird, D. W., Sosinsky, G. E., Tsien, R. Y., & Ellisman, M. H. (2002) · Science · doi:10.1126/science.1068793 · Cited by 907
A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
Lukinavičius, G., Umezawa, K., Olivier, N., Honigmann, A., Yang, G., Plass, T., Mueller, V., Reymond, L., Corrêa Jr, I. R., Luo, Z.-G., et al. (2013) · Nature Chemistry · doi:10.1038/nchem.1546 · Cited by 893
Fluorescence lifetime imaging – techniques and applications
BECKER, W. (2012) · Journal of Microscopy · doi:10.1111/j.1365-2818.2012.03618.x · Cited by 890
Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure
Shtengel, G., Galbraith, J. A., Galbraith, C. G., Lippincott-Schwartz, J., Gillette, J. M., Manley, S., Sougrat, R., Waterman, C. M., Kanchanawong, P., Davidson, M. W., et al. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0813131106 · Cited by 888
Methods of single-molecule fluorescence spectroscopy and microscopy
Moerner, W. E., & Fromm, D. P. (2003) · Review of Scientific Instruments · doi:10.1063/1.1589587 · Cited by 881
Subdiffraction resolution in far-field fluorescence microscopy
Klar, T. A., & Hell, S. W. (1999) · Optics Letters · doi:10.1364/ol.24.000954 · Cited by 864
Triplet–Triplet Absorption Spectra of Organic Molecules in Condensed Phases
Carmichael, I., & Hug, G. L. (1986) · Journal of Physical and Chemical Reference Data · doi:10.1063/1.555770 · Cited by 849
Hofmann, M., Eggeling, C., Jakobs, S., & Hell, S. W. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506010102 · Cited by 844
XXXVIII.A suggested method for extending microscopic resolution into the ultra-microscopic region
Synge, E. H. (1928) · The London, Edinburgh, and Dublin Philosophical Magazine and Journal of Science · doi:10.1080/14786440808564615 · Cited by 843
Cell-substrate contacts illuminated by total internal reflection fluorescence.
Axelrod, D. (1981) · The Journal of cell biology · doi:10.1083/jcb.89.1.141 · Cited by 832
Fluorogenic probes for live-cell imaging of the cytoskeleton
Lukinavičius, G., Reymond, L., D'Este, E., Masharina, A., Göttfert, F., Ta, H., Güther, A., Fournier, M., Rizzo, S., Waldmann, H., et al. (2014) · Nature Methods · doi:10.1038/nmeth.2972 · Cited by 820
STED microscopy reveals crystal colour centres with nanometric resolution
Rittweger, E., Han, K. Y., Irvine, S. E., Eggeling, C., & Hell, S. W. (2009) · Nature Photonics · doi:10.1038/nphoton.2009.2 · Cited by 818
Video-Rate Far-Field Optical Nanoscopy Dissects Synaptic Vesicle Movement
Westphal, V., Rizzoli, S. O., Lauterbach, M. A., Kamin, D., Jahn, R., & Hell, S. W. (2008) · Science · doi:10.1126/science.1154228 · Cited by 788
Bruchpilot Promotes Active Zone Assembly, Ca 2+ Channel Clustering, and Vesicle Release
Kittel, R. J., Wichmann, C., Rasse, T. M., Fouquet, W., Schmidt, M., Schmid, A., Wagh, D. A., Pawlu, C., Kellner, R. R., Willig, K. I., et al. (2006) · Science · doi:10.1126/science.1126308 · Cited by 772
Theory and Practice of Scanning Optical Microscopy
Dainty, J. C. (1985) · Optica Acta: International Journal of Optics · doi:10.1080/713821694 · Cited by 766
Fluorescence nanoscopy by ground-state depletion and single-molecule return
Fölling, J., Bossi, M., Bock, H., Medda, R., Wurm, C. A., Hein, B., Jakobs, S., Eggeling, C., & Hell, S. W. (2008) · Nature Methods · doi:10.1038/nmeth.1257 · Cited by 758
Development of a 500 Å spatial resolution light microscope
Lewis, A., Isaacson, M., Harootunian, A., & Muray, A. (1984) · Ultramicroscopy · doi:10.1016/0304-3991(84)90201-8 · Cited by 757
Metallized tip amplification of near-field Raman scattering
Hayazawa, N., Inouye, Y., Sekkat, Z., & Kawata, S. (2000) · Optics Communications · doi:10.1016/s0030-4018(00)00894-4 · Cited by 730
Eggeling, C., Widengren, J., Rigler, R., & Seidel, C. A. M. (1998) · Analytical Chemistry · doi:10.1021/ac980027p · Cited by 718
Self-citationLocally enhanced Raman spectroscopy with an atomic force microscope
Anderson, M. S. (2000) · Applied Physics Letters · doi:10.1063/1.126546 · Cited by 718
The Photon Counting Histogram in Fluorescence Fluctuation Spectroscopy
Chen, Y., Müller, J. D., So, P. T. C., & Gratton, E. (1999) · Biophysical Journal · doi:10.1016/s0006-3495(99)76912-2 · Cited by 682
The Demonstration of Pneumococcal Antigen in Tissues by the Use of Fluorescent Antibody
Coons, A. H., Creech, H. J., Jones, R. N., & Berliner, E. (1942) · The Journal of Immunology · doi:10.4049/jimmunol.45.3.159 · Cited by 673
Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm
Albota, M. A., Xu, C., & Webb, W. W. (1998) · Applied Optics · doi:10.1364/ao.37.007352 · Cited by 672
Photophysics of Fluorescent Probes for Single-Molecule Biophysics and Super-Resolution Imaging
Ha, T., & Tinnefeld, P. (2012) · Annual Review of Physical Chemistry · doi:10.1146/annurev-physchem-032210-103340 · Cited by 672
Fluorescent indicators for cytosolic sodium
Minta, A., & Tsien, R. Y. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)47321-3 · Cited by 658
Nitrogen-vacancy center in diamond: Model of the electronic structure and associated dynamics
Manson, N. B., Harrison, J. P., & Sellars, M. J. (2006) · Physical Review B · doi:10.1103/physrevb.74.104303 · Cited by 650
Kapanidis, A. N., Lee, N. K., Laurence, T. A., Doose, S., Margeat, E., & Weiss, S. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0401690101 · Cited by 630
A Reducing and Oxidizing System Minimizes Photobleaching and Blinking of Fluorescent Dyes
Vogelsang, J., Kasper, R., Steinhauer, C., Person, B., Heilemann, M., Sauer, M., & Tinnefeld, P. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200801518 · Cited by 618
Membrane protein sequestering by ionic protein–lipid interactions
van den Bogaart, G., Meyenberg, K., Risselada, H. J., Amin, H., Willig, K. I., Hubrich, B. E., Dier, M., Hell, S. W., Grubmüller, H., Diederichsen, U., et al. (2011) · Nature · doi:10.1038/nature10545 · Cited by 617
Nair, D., Hosy, E., Petersen, J. D., Constals, A., Giannone, G., Choquet, D., & Sibarita, J.-B. (2013) · Journal of Neuroscience · doi:10.1523/jneurosci.2381-12.2013 · Cited by 617
Laterally modulated excitation microscopy: improvement of resolution by using a diffraction grating:
Heintzmann, R., & Cremer, C. G. (1999) · SPIE Proceedings · doi:10.1117/12.336833 · Cited by 589
Localization Accuracy in Single-Molecule Microscopy
Ober, R. J., Ram, S., & Ward, E. S. (2004) · Biophysical Journal · doi:10.1016/s0006-3495(04)74193-4 · Cited by 587
Müller, B. K., Zaychikov, E., Bräuchle, C., & Lamb, D. C. (2005) · Biophysical Journal · doi:10.1529/biophysj.105.064766 · Cited by 568
Saturated patterned excitation microscopy—a concept for optical resolution improvement
Heintzmann, R., Jovin, T. M., & Cremer, C. (2002) · Journal of the Optical Society of America A · doi:10.1364/josaa.19.001599 · Cited by 566
Properties of a 4Pi confocal fluorescence microscope
Hell, S., & Stelzer, E. H. K. (1992) · Journal of the Optical Society of America A · doi:10.1364/josaa.9.002159 · Cited by 560
XXX. On the change of refrangibility of light
Stokes, G. G. (1852) · Philosophical Transactions of the Royal Society of London · doi:10.1098/rstl.1852.0022 · Cited by 541
Single-molecule microscopy on model membranes reveals anomalous diffusion
Schütz, G. J., Schindler, H., & Schmidt, T. (1997) · Biophysical Journal · doi:10.1016/s0006-3495(97)78139-6 · Cited by 540
Hell, S. W., & Kroug, M. (1995) · Applied Physics B Lasers and Optics · doi:10.1007/bf01081333 · Cited by 532
Photobleaching in Two-Photon Excitation Microscopy
Patterson, G. H., & Piston, D. W. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76762-2 · Cited by 527
Macromolecular-scale resolution in biological fluorescence microscopy
Donnert, G., Keller, J., Medda, R., Andrei, M. A., Rizzoli, S. O., Lührmann, R., Jahn, R., Eggeling, C., & Hell, S. W. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604965103 · Cited by 527
Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms
Huang, F., Hartwich, T. M. P., Rivera-Molina, F. E., Lin, Y., Duim, W. C., Long, J. J., Uchil, P. D., Myers, J. R., Baird, M. A., Mothes, W., et al. (2013) · Nature Methods · doi:10.1038/nmeth.2488 · Cited by 526
STED microscopy with continuous wave beams
Willig, K. I., Harke, B., Medda, R., & Hell, S. W. (2007) · Nature Methods · doi:10.1038/nmeth1108 · Cited by 523
Müller, C. B., & Enderlein, J. (2010) · Physical Review Letters · doi:10.1103/physrevlett.104.198101 · Cited by 521
Nanoscale Resolution in the Focal Plane of an Optical Microscope
Westphal, V., & Hell, S. W. (2005) · Physical Review Letters · doi:10.1103/physrevlett.94.143903 · Cited by 502
Diffraction-unlimited all-optical imaging and writing with a photochromic GFP
Grotjohann, T., Testa, I., Leutenegger, M., Bock, H., Urban, N. T., Lavoie-Cardinal, F., Willig, K. I., Eggeling, C., Jakobs, S., & Hell, S. W. (2011) · Nature · doi:10.1038/nature10497 · Cited by 501
Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes
Schwille, P., Korlach, J., & Webb, W. W. (1999) · Cytometry · doi:10.1002/(sici)1097-0320(19990701)36:3<176::aid-cyto5>3.0.co;2-f · Cited by 487
Detection of single fluorescent molecules
Brooks Shera, E., Seitzinger, N. K., Davis, L. M., Keller, R. A., & Soper, S. A. (1990) · Chemical Physics Letters · doi:10.1016/0009-2614(90)85485-u · Cited by 477
VOIE, A. H., BURNS, D. H., & SPELMAN, F. A. (1993) · Journal of Microscopy · doi:10.1111/j.1365-2818.1993.tb03346.x · Cited by 477
Hein, B., Willig, K. I., & Hell, S. W. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0807705105 · Cited by 475
Anatomy and Dynamics of a Supramolecular Membrane Protein Cluster
Sieber, J. J., Willig, K. I., Kutzner, C., Gerding-Reimers, C., Harke, B., Donnert, G., Rammner, B., Eggeling, C., Hell, S. W., Grubmüller, H., et al. (2007) · Science · doi:10.1126/science.1141727 · Cited by 461
Kask, P., Palo, K., Ullmann, D., & Gall, K. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.24.13756 · Cited by 455
Proposed method for molecular optical imaging
Betzig, E. (1995) · Optics Letters · doi:10.1364/ol.20.000237 · Cited by 444
Resolution scaling in STED microscopy
Harke, B., Keller, J., Ullal, C. K., Westphal, V., Schönle, A., & Hell, S. W. (2008) · Optics Express · doi:10.1364/oe.16.004154 · Cited by 438
Hoebe, R. A., Van Oven, C. H., Gadella, T. W. J., Dhonukshe, P. B., Van Noorden, C. J. F., & Manders, E. M. M. (2007) · Nature Biotechnology · doi:10.1038/nbt1278 · Cited by 435
Wawrezinieck, L., Rigneault, H., Marguet, D., & Lenne, P.-F. (2005) · Biophysical Journal · doi:10.1529/biophysj.105.067959 · Cited by 431
Spherical nanosized focal spot unravels the interior of cells
Schmidt, R., Wurm, C. A., Jakobs, S., Engelhardt, J., Egner, A., & Hell, S. W. (2008) · Nature Methods · doi:10.1038/nmeth.1214 · Cited by 428
Sharper low-power STED nanoscopy by time gating
Vicidomini, G., Moneron, G., Han, K. Y., Westphal, V., Ta, H., Reuss, M., Engelhardt, J., Eggeling, C., & Hell, S. W. (2011) · Nature Methods · doi:10.1038/nmeth.1624 · Cited by 423
Maturation of active zone assembly by Drosophila Bruchpilot
Fouquet, W., Owald, D., Wichmann, C., Mertel, S., Depner, H., Dyba, M., Hallermann, S., Kittel, R. J., Eimer, S., & Sigrist, S. J. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200812150 · Cited by 406
Cellular Traction Stresses Increase with Increasing Metastatic Potential
Kraning-Rush, C. M., Califano, J. P., & Reinhart-King, C. A. (2012) · PLoS ONE · doi:10.1371/journal.pone.0032572 · Cited by 400
Filming Biomolecular Processes by High-Speed Atomic Force Microscopy
Ando, T., Uchihashi, T., & Scheuring, S. (2014) · Chemical Reviews · doi:10.1021/cr4003837 · Cited by 392
XV. On the theory of optical images, with special reference to the microscope
Rayleigh (1896) · The London Edinburgh and Dublin Philosophical Magazine and Journal of Science · doi:10.1080/14786449608620902 · Cited by 386
Live-cell 3D super-resolution imaging in thick biological samples
Cella Zanacchi, F., Lavagnino, Z., Perrone Donnorso, M., Del Bue, A., Furia, L., Faretta, M., & Diaspro, A. (2011) · Nature Methods · doi:10.1038/nmeth.1744 · Cited by 385
Lee, S.-H., Shin, J. Y., Lee, A., & Bustamante, C. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1215175109 · Cited by 379
Petersen, N. O., Höddelius, P., Wiseman, P. W., Seger, O., & Magnusson, K. (1993) · Biophysical Journal · doi:10.1016/s0006-3495(93)81173-1 · Cited by 377
Nanoscale resolution in GFP-based microscopy
Willig, K. I., Kellner, R. R., Medda, R., Hein, B., Jakobs, S., & Hell, S. W. (2006) · Nature Methods · doi:10.1038/nmeth922 · Cited by 372
Single-molecule high-resolution imaging with photobleaching
Gordon, M. P., Ha, T., & Selvin, P. R. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0401638101 · Cited by 372
Immunolabelling for electron microscopy
(1983) · Regulatory Peptides · doi:10.1016/0167-0115(83)90146-5 · Cited by 370
Rotational brownian motion and fluorescence intensify fluctuations
Ehrenberg, M., & Rigler, R. (1974) · Chemical Physics · doi:10.1016/0301-0104(74)85005-6 · Cited by 366
Hsiao, W. W.-W., Hui, Y. Y., Tsai, P.-C., & Chang, H.-C. (2016) · Accounts of Chemical Research · doi:10.1021/acs.accounts.5b00484 · Cited by 365
Focal Spots of Size λ / 23 Open Up Far-Field Florescence Microscopy at 33 nm Axial Resolution
Dyba, M., & Hell, S. W. (2002) · Physical Review Letters · doi:10.1103/physrevlett.88.163901 · Cited by 364
Nanoscopy in a living mouse brain
Berning, S., Willig, K. I., Steffens, H., Dibaj, P., & Hell, S. W. (2012) · Science · doi:10.1126/science.1215369 · Cited by 363
Protein localization in electron micrographs using fluorescence nanoscopy
Watanabe, S., Punge, A., Hollopeter, G., Willig, K. I., Hobson, R. J., Davis, M. W., Hell, S. W., & Jorgensen, E. M. (2011) · Nature Methods · doi:10.1038/nmeth.1537 · Cited by 351
Cyanine fluorophore derivatives with enhanced photostability
Altman, R. B., Terry, D. S., Zhou, Z., Zheng, Q., Geggier, P., Kolster, R. A., Zhao, Y., Javitch, J. A., Warren, J. D., & Blanchard, S. C. (2012) · Nature Methods · doi:10.1038/nmeth.1774 · Cited by 339
Imaging mass spectrometry in microbiology
Watrous, J. D., & Dorrestein, P. C. (2011) · Nature Reviews Microbiology · doi:10.1038/nrmicro2634 · Cited by 338
Fluorescent proteins for live-cell imaging with super-resolution
Nienhaus, K., & Ulrich Nienhaus, G. (2014) · Chem. Soc. Rev. · doi:10.1039/c3cs60171d · Cited by 337
Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP
Biteen, J. S., Thompson, M. A., Tselentis, N. K., Bowman, G. R., Shapiro, L., & Moerner, W. E. (2008) · Nature Methods · doi:10.1038/nmeth.1258 · Cited by 337
The 2015 super-resolution microscopy roadmap
Hell, S. W., Sahl, S. J., Bates, M., Zhuang, X., Heintzmann, R., Booth, M. J., Bewersdorf, J., Shtengel, G., Hess, H., Tinnefeld, P., et al. (2015) · Journal of Physics D: Applied Physics · doi:10.1088/0022-3727/48/44/443001 · Cited by 325
Nanoscale Chemical Imaging Using Tip‐Enhanced Raman Spectroscopy: A Critical Review
Schmid, T., Opilik, L., Blum, C., & Zenobi, R. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201203849 · Cited by 323
Photophysical processes in single molecule organic fluorescent probes
Stennett, E. M. S., Ciuba, M. A., & Levitus, M. (2014) · Chem. Soc. Rev. · doi:10.1039/c3cs60211g · Cited by 323
Šišková, Z., Justus, D., Kaneko, H., Friedrichs, D., Henneberg, N., Beutel, T., Pitsch, J., Schoch, S., Becker, A., von der Kammer, H., et al. (2014) · Neuron · doi:10.1016/j.neuron.2014.10.024 · Cited by 320
STED Nanoscopy Reveals the Ubiquity of Subcortical Cytoskeleton Periodicity in Living Neurons
D’Este, E., Kamin, D., Göttfert, F., El-Hady, A., & Hell, S. W. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.02.007 · Cited by 316
Adaptive optics enables 3D STED microscopy in aberrating specimens
Gould, T. J., Burke, D., Bewersdorf, J., & Booth, M. J. (2012) · Optics Express · doi:10.1364/oe.20.020998 · Cited by 315
Membrane molecules mobile even after chemical fixation
Tanaka, K. A. K., Suzuki, K. G. N., Shirai, Y. M., Shibutani, S. T., Miyahara, M. S. H., Tsuboi, H., Yahara, M., Yoshimura, A., Mayor, S., Fujiwara, T. K., et al. (2010) · Nature Methods · doi:10.1038/nmeth.f.314 · Cited by 315
RIM-Binding Protein, a Central Part of the Active Zone, Is Essential for Neurotransmitter Release
Liu, K. S. Y., Siebert, M., Mertel, S., Knoche, E., Wegener, S., Wichmann, C., Matkovic, T., Muhammad, K., Depner, H., Mettke, C., et al. (2011) · Science · doi:10.1126/science.1212991 · Cited by 306
Coaligned Dual-Channel STED Nanoscopy and Molecular Diffusion Analysis at 20 nm Resolution
Göttfert, F., Wurm, C. A., Mueller, V., Berning, S., Cordes, V. C., Honigmann, A., & Hell, S. W. (2013) · Biophysical Journal · doi:10.1016/j.bpj.2013.05.029 · Cited by 298
STED Nanoscopy of Actin Dynamics in Synapses Deep Inside Living Brain Slices
Urban, N. T., Willig, K. I., Hell, S. W., & Nägerl, U. V. (2011) · Biophysical Journal · doi:10.1016/j.bpj.2011.07.027 · Cited by 297
A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching
Brakemann, T., Stiel, A. C., Weber, G., Andresen, M., Testa, I., Grotjohann, T., Leutenegger, M., Plessmann, U., Urlaub, H., Eggeling, C., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1952 · Cited by 297
Honigmann, A., Mueller, V., Ta, H., Schoenle, A., Sezgin, E., Hell, S. W., & Eggeling, C. (2014) · Nature Communications · doi:10.1038/ncomms6412 · Cited by 291
2,2′‐Thiodiethanol: A new water soluble mounting medium for high resolution optical microscopy
Staudt, T., Lang, M. C., Medda, R., Engelhardt, J., & Hell, S. W. (2007) · Microscopy Research and Technique · doi:10.1002/jemt.20396 · Cited by 291
Major signal increase in fluorescence microscopy through dark-state relaxation
Donnert, G., Eggeling, C., & Hell, S. W. (2007) · Nature Methods · doi:10.1038/nmeth986 · Cited by 291
Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
Egner, A., Geisler, C., von Middendorff, C., Bock, H., Wenzel, D., Medda, R., Andresen, M., Stiel, A. C., Jakobs, S., Eggeling, C., et al. (2007) · Biophysical Journal · doi:10.1529/biophysj.107.112201 · Cited by 284
Nanoclustering as a dominant feature of plasma membrane organization
Garcia-Parajo, M. F., Cambi, A., Torreno-Pina, J. A., Thompson, N., & Jacobson, K. (2014) · Journal of Cell Science · doi:10.1242/jcs.146340 · Cited by 284
STED microscopy with a supercontinuum laser source
Wildanger, D., Rittweger, E., Kastrup, L., & Hell, S. W. (2008) · Optics Express · doi:10.1364/oe.16.009614 · Cited by 277
A compact STED microscope providing 3D nanoscale resolution
Wildanger, D., Medda, R., Kastrup, L., & Hell, S. W. (2009) · Journal of Microscopy · doi:10.1111/j.1365-2818.2009.03188.x · Cited by 276
Nanoscopy with more than 100,000 'doughnuts'
Chmyrov, A., Keller, J., Grotjohann, T., Ratz, M., d'Este, E., Jakobs, S., Eggeling, C., & Hell, S. W. (2013) · Nature Methods · doi:10.1038/nmeth.2556 · Cited by 271
Stadler, C., Rexhepaj, E., Singan, V. R., Murphy, R. F., Pepperkok, R., Uhlén, M., Simpson, J. C., & Lundberg, E. (2013) · Nature Methods · doi:10.1038/nmeth.2377 · Cited by 265
Brakenhoff, G. J., van der Voort, H. T. M., van Spronsen, E. A., Linnemans, W. A. M., & Nanninga, N. (1985) · Nature · doi:10.1038/317748a0 · Cited by 256
Two-Color Far-Field Fluorescence Nanoscopy
Donnert, G., Keller, J., Wurm, C. A., Rizzoli, S. O., Westphal, V., Schönle, A., Jahn, R., Jakobs, S., Eggeling, C., & Hell, S. W. (2007) · Biophysical Journal · doi:10.1529/biophysj.107.104497 · Cited by 256
Stiel, A. C., Trowitzsch, S., Weber, G., Andresen, M., Eggeling, C., Hell, S. W., Jakobs, S., & Wahl, M. C. (2007) · Biochemical Journal · doi:10.1042/bj20061401 · Cited by 256
Mueller, V., Ringemann, C., Honigmann, A., Schwarzmann, G., Medda, R., Leutenegger, M., Polyakova, S., Belov, V. N., Hell, S. W., & Eggeling, C. (2011) · Biophysical Journal · doi:10.1016/j.bpj.2011.09.006 · Cited by 253
Super-resolution Microscopy Approaches for Live Cell Imaging
Godin, A. G., Lounis, B., & Cognet, L. (2014) · Biophysical Journal · doi:10.1016/j.bpj.2014.08.028 · Cited by 247
Multi-target spectrally resolved fluorescence lifetime imaging microscopy
Niehörster, T., Löschberger, A., Gregor, I., Krämer, B., Rahn, H.-J., Patting, M., Koberling, F., Enderlein, J., & Sauer, M. (2016) · Nature Methods · doi:10.1038/nmeth.3740 · Cited by 243
Yuste, R. (2010) · doi:10.7551/mitpress/9780262013505.001.0001 · Cited by 241
True optical resolution beyond the Rayleigh limit achieved by standing wave illumination
Frohn, J., Knapp, H. F., & Stemmer, A. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.130181797 · Cited by 240
Two-photon excitation STED microscopy
Moneron, G., & Hell, S. W. (2009) · Optics Express · doi:10.1364/oe.17.014567 · Cited by 238
Kopek, B. G., Shtengel, G., Xu, C. S., Clayton, D. A., & Hess, H. F. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1121558109 · Cited by 238
Strategies to Improve Photostabilities in Ultrasensitive Fluorescence Spectroscopy
Widengren, J., Chmyrov, A., Eggeling, C., Löfdahl, P.-Å., & Seidel, C. A. M. (2007) · The Journal of Physical Chemistry A · doi:10.1021/jp0646325 · Cited by 236
Self-citationSuperresolution by image scanning microscopy using pixel reassignment
Sheppard, C. J. R., Mehta, S. B., & Heintzmann, R. (2013) · Optics Letters · doi:10.1364/ol.38.002889 · Cited by 234
Fluorescence Fluctuation Spectroscopy in Subdiffraction Focal Volumes
Kastrup, L., Blom, H., Eggeling, C., & Hell, S. W. (2005) · Physical Review Letters · doi:10.1103/physrevlett.94.178104 · Cited by 229
Self-citationPhotocontrollable Fluorescent Proteins for Superresolution Imaging
Shcherbakova, D. M., Sengupta, P., Lippincott-Schwartz, J., & Verkhusha, V. V. (2014) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-051013-022836 · Cited by 228
Sednev, M. V., Belov, V. N., & Hell, S. W. (2015) · Methods and Applications in Fluorescence · doi:10.1088/2050-6120/3/4/042004 · Cited by 226
Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses
Bückers, J., Wildanger, D., Vicidomini, G., Kastrup, L., & Hell, S. W. (2011) · Optics Express · doi:10.1364/oe.19.003130 · Cited by 225
Dittrich, P. S., & Schwille, P. (2001) · Applied Physics B · doi:10.1007/s003400100737 · Cited by 223
Digman, M. A., Sengupta, P., Wiseman, P. W., Brown, C. M., Horwitz, A. R., & Gratton, E. (2005) · Biophysical Journal · doi:10.1529/biophysj.105.061788 · Cited by 221
Two-colour live-cell nanoscale imaging of intracellular targets
Bottanelli, F., Kromann, E. B., Allgeyer, E. S., Erdmann, R. S., Wood Baguley, S., Sirinakis, G., Schepartz, A., Baddeley, D., Toomre, D. K., Rothman, J. E., et al. (2016) · Nature Communications · doi:10.1038/ncomms10778 · Cited by 221
Widengren, J., Kudryavtsev, V., Antonik, M., Berger, S., Gerken, M., & Seidel, C. A. M. (2006) · Analytical Chemistry · doi:10.1021/ac0522759 · Cited by 217
Self-citationFixation-resistant photoactivatable fluorescent proteins for CLEM
Paez-Segala, M. G., Sun, M. G., Shtengel, G., Viswanathan, S., Baird, M. A., Macklin, J. J., Patel, R., Allen, J. R., Howe, E. S., Piszczek, G., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3225 · Cited by 212
STED nanoscopy with fluorescent quantum dots
Hanne, J., Falk, H. J., Görlitz, F., Hoyer, P., Engelhardt, J., Sahl, S. J., & Hell, S. W. (2015) · Nature Communications · doi:10.1038/ncomms8127 · Cited by 211
Mitigating Unwanted Photophysical Processes for Improved Single-Molecule Fluorescence Imaging
Dave, R., Terry, D. S., Munro, J. B., & Blanchard, S. C. (2009) · Biophysical Journal · doi:10.1016/j.bpj.2008.11.061 · Cited by 210
Schneider, A., Rajendran, L., Honsho, M., Gralle, M., Donnert, G., Wouters, F., Hell, S. W., & Simons, M. (2008) · The Journal of Neuroscience · doi:10.1523/jneurosci.5345-07.2008 · Cited by 210
Simultaneous, accurate measurement of the 3D position and orientation of single molecules
Backlund, M. P., Lew, M. D., Backer, A. S., Sahl, S. J., Grover, G., Agrawal, A., Piestun, R., & Moerner, W. E. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1216687109 · Cited by 203
Hell, S. W., Jakobs, S., & Kastrup, L. (2003) · Applied Physics A: Materials Science & Processing · doi:10.1007/s00339-003-2292-4 · Cited by 201
Superresolution Microscopy on the Basis of Engineered Dark States
Steinhauer, C., Forthmann, C., Vogelsang, J., & Tinnefeld, P. (2008) · Journal of the American Chemical Society · doi:10.1021/ja806590m · Cited by 200
3-Dimensional super-resolution by spectrally selective imaging
van Oijen, A. M., Köhler, J., Schmidt, J., Müller, M., & Brakenhoff, G. J. (1998) · Chemical Physics Letters · doi:10.1016/s0009-2614(98)00673-3 · Cited by 200
In Situ Cryo-Electron Tomography: A Post-Reductionist Approach to Structural Biology
Asano, S., Engel, B. D., & Baumeister, W. (2016) · Journal of Molecular Biology · doi:10.1016/j.jmb.2015.09.030 · Cited by 198
Wagner, E., Lauterbach, M. A., Kohl, T., Westphal, V., Williams, G. S. B., Steinbrecher, J. H., Streich, J.-H., Korff, B., Tuan, H.-T. M., Hagen, B., et al. (2012) · Circulation Research · doi:10.1161/circresaha.112.274530 · Cited by 196
Polarization effect on position accuracy of fluorophore localization
Enderlein, J., Toprak, E., & Selvin, P. R. (2006) · Optics Express · doi:10.1364/oe.14.008111 · Cited by 195
Three-Dimensional Nanoscopy of Colloidal Crystals
Harke, B., Ullal, C. K., Keller, J., & Hell, S. W. (2008) · Nano Letters · doi:10.1021/nl073164n · Cited by 195
Fujiwara, T. K., Iwasawa, K., Kalay, Z., Tsunoyama, T. A., Watanabe, Y., Umemura, Y. M., Murakoshi, H., Suzuki, K. G. N., Nemoto, Y. L., Morone, N., et al. (2016) · Molecular Biology of the Cell · doi:10.1091/mbc.e15-04-0186 · Cited by 195
The SNARE Motif Is Essential for the Formation of Syntaxin Clusters in the Plasma Membrane
Sieber, J. J., Willig, K. I., Heintzmann, R., Hell, S. W., & Lang, T. (2006) · Biophysical Journal · doi:10.1529/biophysj.105.079574 · Cited by 193
Auksorius, E., Boruah, B. R., Dunsby, C., Lanigan, P. M. P., Kennedy, G., Neil, M. A. A., & French, P. M. W. (2008) · Optics Letters · doi:10.1364/ol.33.000113 · Cited by 190
Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids
Sahl, S. J., Leutenegger, M., Hilbert, M., Hell, S. W., & Eggeling, C. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0912894107 · Cited by 187
Aptamers as potential tools for super-resolution microscopy
Opazo, F., Levy, M., Byrom, M., Schäfer, C., Geisler, C., Groemer, T. W., Ellington, A. D., & Rizzoli, S. O. (2012) · Nature Methods · doi:10.1038/nmeth.2179 · Cited by 182
A readily retrievable pool of synaptic vesicles
Hua, Y., Sinha, R., Thiel, C. S., Schmidt, R., Hüve, J., Martens, H., Hell, S. W., Egner, A., & Klingauf, J. (2011) · Nature Neuroscience · doi:10.1038/nn.2838 · Cited by 180
Ding, J. B., Takasaki, K. T., & Sabatini, B. L. (2009) · Neuron · doi:10.1016/j.neuron.2009.07.011 · Cited by 177
Strategy for far-field optical imaging and writing without diffraction limit
Hell, S. W. (2004) · Physics Letters A · doi:10.1016/j.physleta.2004.03.082 · Cited by 177
Puchner, E. M., Walter, J. M., Kasper, R., Huang, B., & Lim, W. A. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1309676110 · Cited by 177
Fluorescence Lifetime Correlation Spectroscopy
Kapusta, P., Wahl, M., Benda, A., Hof, M., & Enderlein, J. (2006) · Journal of Fluorescence · doi:10.1007/s10895-006-0145-1 · Cited by 176
SPDM: light microscopy with single-molecule resolution at the nanoscale
Lemmer, P., Gunkel, M., Baddeley, D., Kaufmann, R., Urich, A., Weiland, Y., Reymann, J., Müller, P., Hausmann, M., & Cremer, C. (2008) · Applied Physics B · doi:10.1007/s00340-008-3152-x · Cited by 174
Bodzon‐Kulakowska, A., & Suder, P. (2016) · Mass Spectrometry Reviews · doi:10.1002/mas.21468 · Cited by 173
Cited by
Present Application and Perspectives of Organoid Imaging Technology
Fei, K., Zhang, J., Yuan, J., & Xiao, P. (2022) · Bioengineering · doi:10.3390/bioengineering9030121 · Cited by 65
Metadata
- Authors
- Blom, H., & Widengren, J.
- Venue
- Chemical Reviews
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-03-06
- Volume
- 117
- Issue
- 11
- Pages
- 7377-7427
- ISSN
- 0009-2665 (ISSN-L)
- 1520-6890
- OpenAlex
- W2594822719
- Cited by
- 339
- References
- 320
- FWCI
- 2.30
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
