Flavonoids from Whole-Grain Oat Alleviated High-Fat Diet-Induced Hyperlipidemia via Regulating Bile Acid Metabolism and Gut Microbiota in Mice
Abstract
A high-fat diet (HFD) causes hyperlipidemia, which worsens disturbances in bile acid (BA) metabolism and gut microbiota. This study aimed to investigate the regulation of flavonoids from whole-grain oat (FO) on BA metabolism and gut microbiota in HFD-induced hyperlipidemic mice. The experiment results showed that FO improved serum lipid profiles and decreased body weight and lipid deposition in HFD-fed mice. Through real-time qualitative polymerase chain reaction (RT-qPCR) and Western blot assays, by up-regulating the expression of PPARα, CPT-1, CYP7A1, FXR, TGR5, NTCP, and BSTP, and down-regulating those of SREBP-1c, FAS, and ASBT, FO suppressed lipogenesis, promoted lipolysis and BA synthesis, and efflux to faeces via the FXR pathway. 16s rRNA sequencing revealed that FO significantly increased Akkermansia and significantly decreased Lachnoclostridium, Blautia, Colidextribacter, and Desulfovibrio . Spearman’s correlation analysis showed that these bacteria were strongly correlated with hyperlipidemia-related parameters. Therefore, our results indicated that FO possessed an antihyperlipidemic effect via regulating the gut–liver axis, i.e., BA metabolism and gut microbiota.
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Blautia has been found to decrease the elevated cholesterol levels caused by a high-fat diet in a mouse model with high cholesterol levels by regulating the metabolism of bile acids.
“Blautia has been found to decrease the elevated cholesterol levels caused by a high-fat diet in a mouse model with high cholesterol levels by regulating the metabolism of bile acids (Duan et al., 2021).”
From Lactiplantibacillus plantarum N4 ameliorates lipid metabolism and gut microbiota structure in high fat diet-fed rats (2024) · cites this paper as Duan et al., 2021
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- Authors
- Duan, R., Guan, X., Huang, K., Zhang, Y., Li, S., Xia, J., & Shen, M.
- Affiliations
- University of Shanghai for Science and Technology (CN)
- School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
- Corresponding author
- Guan, Xiao
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2021-07-02
- Volume
- 69
- Issue
- 27
- Pages
- 7629-7640
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W3173638043
- Cited by
- 198
- References
- 52
- Citations by year
- 2026: 19 · 2025: 38 · 2024: 60 · 2023: 39 · 2022: 38 · 2021: 4
- FWCI
- 11.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Capacity-Building Project of Local Universities of SSTC (20060502100)
- National Natural Science Foundation of China (31901609)
- Dawn Program of Shanghai Education Commission (19SG45)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
- Health Sciences › Medicine › Surgery › Natural Products and Biological Research
- Keywords
- whole grain oats, flavonoids, hyperlipidemia, high-fat diet, bile acid metabolism, gut microbiota, gut-liver axis, FXR signaling, Akkermansia, lipogenesis, lipolysis, lipid profile, hypolipidemic effect
- MeSH terms
- Gastrointestinal Microbiome, Animals, Bile Acids and Salts, Flavonoids, Hyperlipidemias, Mice, Inbred C57BL, RNA, Ribosomal, 16S, Avena, Lipid Metabolism, Mice, Diet, High-Fat
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