Cited by 50Tea Polyphenols and EffectsAutophagy in Disease and TherapyNeuroinflammation and Neurodegeneration Mechanisms

Green Tea Catechin Prevents Hypoxia/Reperfusion-Evoked Oxidative Stress-Regulated Autophagy-Activated Apoptosis and Cell Death in Microglial Cells

Chen, Chang-Mu, Wu, Cheng-Tien, Yang, Ting-Hua, Chang, Ya-An, Sheu, Meei-Ling, Liu, Shing Hwa·2016·Journal of Agricultural and Food Chemistry

Abstract

Defective activation and proliferation in microglial cells has been suggested to be associated with the increase of cerebral ischemia/reperfusion injury. We investigated the protection and molecular mechanism of green tea catechin on hypoxia/reperfusion-induced microglial cell injury in vitro. Microglial cells were cultured in hypoxia condition (O2 < 1%) and then re-incubated to the complete normal culture medium (reperfusion). Hypoxia/reperfusion obviously decreased cell viability and induced apoptosis in microglial cells, but not in neuronal cells. Catechin significantly inhibited the hypoxia/reperfusion-induced decreased cell viability and increased reactive oxygen species (ROS) and apoptosis in microglia. The administration of both PI3K/Akt inhibitor LY294002 and mTOR inhibitor rapamycin demonstrated that Akt/mTOR-regulated autophagy was involved in the hypoxia/reperfusion-induced microglia apoptosis/death. Catechin up-regulated the Akt and mTOR phosphorylation and inhibited the hypoxia/reperfusion-induced autophagy in microglia. These results suggest that hypoxia/reperfusion can evoke autophagy-activated microglia apoptosis/death via an ROS-regulated Akt/mTOR signaling pathway, which can be reversed by catechin.

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Crossref · 10.1021/acs.jafc.6b01513

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Authors
Chen, C.-M., Wu, C.-T., Yang, T.-H., Chang, Y.-A., Sheu, M.-L., & Liu, S. H.
Affiliations
  • National Taiwan University Hospital (TW)
  • National Taiwan University (TW)
  • National Chung Hsing University (TW)
Corresponding author
Liu, Shing Hwa
Venue
Journal of Agricultural and Food Chemistry
Published
2016-05-10
Volume
64
Issue
20
Pages
4078-4085
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2346106513
Cited by
50
References
44
Citations by year
2026: 1 · 2025: 3 · 2024: 5 · 2023: 6 · 2022: 5 · 2021: 5 · 2020: 7 · 2019: 4 · 2018: 6 · 2017: 7 · 2016: 1
FWCI
5.44
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Science Council of Taiwan (NSC 100-2314-B-002-084)
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Tea Polyphenols and Effects
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
microglia, hypoxia/reoxygenation injury, cerebral ischemia-reperfusion injury, oxidative stress, reactive oxygen species, autophagy, apoptosis, Akt/mTOR signaling, PI3K/Akt, rapamycin, LY294002, neuroprotection
MeSH terms
Hypoxia, Autophagy, Catechin, Cell Survival, Humans, Phosphorylation, Plant Extracts, Signal Transduction, Apoptosis, Reactive Oxygen Species, Microglia, Oxidative Stress, Phosphatidylinositol 3-Kinases, Camellia sinensis, Proto-Oncogene Proteins c-akt, TOR Serine-Threonine Kinases

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