Green Tea Catechin Prevents Hypoxia/Reperfusion-Evoked Oxidative Stress-Regulated Autophagy-Activated Apoptosis and Cell Death in Microglial Cells
Abstract
Defective activation and proliferation in microglial cells has been suggested to be associated with the increase of cerebral ischemia/reperfusion injury. We investigated the protection and molecular mechanism of green tea catechin on hypoxia/reperfusion-induced microglial cell injury in vitro. Microglial cells were cultured in hypoxia condition (O2 < 1%) and then re-incubated to the complete normal culture medium (reperfusion). Hypoxia/reperfusion obviously decreased cell viability and induced apoptosis in microglial cells, but not in neuronal cells. Catechin significantly inhibited the hypoxia/reperfusion-induced decreased cell viability and increased reactive oxygen species (ROS) and apoptosis in microglia. The administration of both PI3K/Akt inhibitor LY294002 and mTOR inhibitor rapamycin demonstrated that Akt/mTOR-regulated autophagy was involved in the hypoxia/reperfusion-induced microglia apoptosis/death. Catechin up-regulated the Akt and mTOR phosphorylation and inhibited the hypoxia/reperfusion-induced autophagy in microglia. These results suggest that hypoxia/reperfusion can evoke autophagy-activated microglia apoptosis/death via an ROS-regulated Akt/mTOR signaling pathway, which can be reversed by catechin.
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The greatest negative impact of reactive oxygen species is formed during oxidative phosphorylation in the mitochondrial crests at the end of the aerobic respiration process.
“The greatest negative impact is formed during oxidative phosphorylation in the mitochondrial crests at the end of the aerobic respiration process [111,112,113,114].”
From Multi-Target Mechanisms of Phytochemicals in Alzheimer’s Disease: Effects on Oxidative Stress, Neuroinflammation and Protein Aggregation (2022) · cites this paper as [114]
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- Authors
- Chen, C.-M., Wu, C.-T., Yang, T.-H., Chang, Y.-A., Sheu, M.-L., & Liu, S. H.
- Affiliations
- National Taiwan University Hospital (TW)
- National Taiwan University (TW)
- National Chung Hsing University (TW)
- Corresponding author
- Liu, Shing Hwa
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2016-05-10
- Volume
- 64
- Issue
- 20
- Pages
- 4078-4085
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2346106513
- Cited by
- 50
- References
- 44
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 5 · 2023: 6 · 2022: 5 · 2021: 5 · 2020: 7 · 2019: 4 · 2018: 6 · 2017: 7 · 2016: 1
- FWCI
- 5.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Council of Taiwan (NSC 100-2314-B-002-084)
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Tea Polyphenols and Effects
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- microglia, hypoxia/reoxygenation injury, cerebral ischemia-reperfusion injury, oxidative stress, reactive oxygen species, autophagy, apoptosis, Akt/mTOR signaling, PI3K/Akt, rapamycin, LY294002, neuroprotection
- MeSH terms
- Hypoxia, Autophagy, Catechin, Cell Survival, Humans, Phosphorylation, Plant Extracts, Signal Transduction, Apoptosis, Reactive Oxygen Species, Microglia, Oxidative Stress, Phosphatidylinositol 3-Kinases, Camellia sinensis, Proto-Oncogene Proteins c-akt, TOR Serine-Threonine Kinases
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