The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Single-Exosome-Counting Immunoassays for Cancer Diagnostics
Abstract
Exosomes shed by tumor cells have been recognized as promising biomarkers for cancer diagnostics due to their unique composition and functions. Quantification of low concentrations of specific exosomes present in very small volumes of clinical samples may be used for noninvasive cancer diagnosis and prognosis. We developed an immunosorbent assay for digital qualification of target exosomes using droplet microfluidics. The exosomes were immobilized on magnetic microbeads through sandwich ELISA complexes tagged with an enzymatic reporter that produces a fluorescent signal. The constructed beads were further isolated and encapsulated into a sufficient number of droplets to ensure only a single bead was encapsulated in a droplet. Our droplet-based single-exosome-counting enzyme-linked immunoassay (droplet digital ExoELISA) approach enables absolute counting of cancer-specific exosomes to achieve unprecedented accuracy. We were able to achieve a limit of detection (LOD) down to 10 enzyme-labeled exosome complexes per microliter (∼10 –17 M). We demonstrated the application of the droplet digital ExoELISA platform in quantitative detection of exosomes in plasma samples directly from breast cancer patients. We believe our approach may have the potential for early diagnosis of cancer and accelerate the discovery of cancer exosomal biomarkers for clinical diagnosis.
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Cites
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Cited by
Exosomes as a new frontier of cancer liquid biopsy
Yu, D., Li, Y., Wang, M., Gu, J., Xu, W., Cai, H., Fang, X., & Zhang, X. (2022) · Molecular Cancer · doi:10.1186/s12943-022-01509-9 · Cited by 855
Using single-vesicle technologies to unravel the heterogeneity of extracellular vesicles
Bordanaba-Florit, G., Royo, F., Kruglik, S. G., & Falcón-Pérez, J. M. (2021) · Nature Protocols · doi:10.1038/s41596-021-00551-z · Cited by 325
Exosomal PD‐L1: New Insights Into Tumor Immune Escape Mechanisms and Therapeutic Strategies
Zhou, K., Guo, S., Li, F., Sun, Q., & Liang, G. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.569219 · Cited by 99
Yang, L., Patel, K. D., Rathnam, C., Thangam, R., Hou, Y., Kang, H., & Lee, K. (2022) · Small · doi:10.1002/smll.202104783 · Cited by 81
Analysis of extracellular vesicles as emerging theranostic nanoplatforms
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Metadata
- Authors
- Liu, C., Xu, X., Li, B., Situ, B., Pan, W., Hu, Y., An, T., Yao, S., & Zheng, L.
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-06-11
- Volume
- 18
- Issue
- 7
- Pages
- 4226-4232
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- OpenAlex
- W2807797873
- Cited by
- 406
- References
- 52
- FWCI
- 11.61
- Type
- journal article
- Language
- en
- Open access
- closed
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