Conjugated Polymer-Based Hybrid Nanoparticles with Two-Photon Excitation and Near-Infrared Emission Features for Fluorescence Bioimaging within the Biological Window
Abstract
Hybrid fluorescent nanoparticles (NPs) capable of fluorescing near-infrared (NIR) light (centered ∼730 nm) upon excitation of 800 nm laser light were constructed. A new type of conjugated polymer with two-photon excited fluorescence (TPEF) feature, P-F8-DPSB, was used as the NIR-light harvesting component and the energy donor while a NIR fluorescent dye, DPA-PR-PDI, was used as the energy acceptor and the NIR-light emitting component for the construction of the fluorescent NPs. The hybrid NPs possess δ value up to 2.3 × 10(6) GM per particle upon excitation of 800 nm pulse laser. The excellent two-photon absorption (TPA) property of the conjugated polymer component, together with its high fluorescence quantum yield (ϕ) up to 45% and the efficient energy transfer from the conjugated polymer to NIR-emitting fluorophore with efficiency up to 90%, imparted the hybrid NPs with TPEF-based NIR-input-NIR-output fluorescence imaging ability with penetration depth up to 1200 μm. The practicability of the hybrid NPs for fluorescence imaging in Hela cells was validated.
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The careful pairing of CP energy donors with suitable energy-acceptor dyes allows one to obtain brighter fluorescence probes due to the signal amplification provided by the energy donor.
“The careful pairing of CP energy donors with suitable energy-acceptor dyes allows one to obtain brighter fluorescence probes due to the signal amplification provided by the energy donor.129,130”
From The Pursuit of Shortwave Infrared-Emitting Nanoparticles with Bright Fluorescence through Molecular Design and Excited-State Engineering of Molecular Aggregates (2022) · cites this paper as [129]
Cites
Principles of Fluorescence Spectroscopy
Lakowicz, J. R. (2006) · Springer eBooks · doi:10.1007/978-0-387-46312-4 · Cited by 19,375 · see citing works
The Fluorescent Toolbox for Assessing Protein Location and Function
Giepmans, B. N. G., Adams, S., Ellisman, M. H., & Tsien, R. Y. (2006) · Science · doi:10.1126/science.1124618 · Cited by 2,782 · see citing works
Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization
Yildiz, A., Forkey, J., McKinney, S., Ha, T., Goldman, Y., & Selvin, P. (2003) · Science · doi:10.1126/science.1084398 · Cited by 1,870 · see citing works
Liu, Q., Guo, B., Rao, Z., Zhang, B., & Gong, J. R. (2013) · Nano Letters · doi:10.1021/nl400368v · Cited by 958 · see citing works
Hong, G., Zou, Y., Antaris, A. L., Diao, S., Wu, D., Cheng, K., Zhang, X., Chen, C., Liu, B., He, Y., et al. (2014) · Nature Communications · doi:10.1038/ncomms5206 · Cited by 563 · see citing works
High-throughput fluorescence microscopy for systems biology
Pepperkok, R., & Ellenberg, J. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1979 · Cited by 412 · see citing works
Novel red-emitting fluorene-based copolymers
Hou, Q., Xu, Y., Yang, W., et al. (2002) · Journal of Materials Chemistry · doi:10.1039/b203862e · Cited by 334 · see citing works
Tao, Z., Hong, G., Shinji, C., Chen, C., Diao, S., Antaris, A. L., Zhang, B., Zou, Y., & Dai, H. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201307346 · Cited by 330 · see citing works
Conjugated Polymer Dots for Multiphoton Fluorescence Imaging
Wu, C., Szymanski, C. J., Cain, Z., & McNeill, J. (2007) · Journal of the American Chemical Society · doi:10.1021/ja074590d · Cited by 323 · see citing works
Benzothiadiazole Derivatives as Fluorescence Imaging Probes: Beyond Classical Scaffolds
Neto, B. A. D., Carvalho, P. H. P. R., & Corrêa, J. R. (2015) · Accounts of Chemical Research · doi:10.1021/ar500468p · Cited by 278 · see citing works
High-resolution three-photon biomedical imaging using doped ZnS nanocrystals
Yu, J. H., Kwon, S., Petrášek, Z., et al. (2013) · Nature Materials · doi:10.1038/nmat3565 · Cited by 276 · see citing works
Wu, C., Zheng, Y., Szymanski, C. J., et al. (2008) · The Journal of Physical Chemistry C · doi:10.1021/jp074149+ · Cited by 257 · see citing works
Electron-Donating Perylene Tetracarboxylic Acids for Dye-Sensitized Solar Cells
Shibano, Y., Umeyama, T., Matano, Y., et al. (2007) · Organic Letters · doi:10.1021/ol070556s · Cited by 253 · see citing works
Tao, Z., Hong, G., Shinji, C., Chen, C., Diao, S., Antaris, A. L., Zhang, B., Zou, Y., & Dai, H. (2013) · Angewandte Chemie · doi:10.1002/ange.201307346 · Cited by 239 · see citing works
Near-Infrared Fluorescent Dye-Doped Semiconducting Polymer Dots
Jin, Y., Ye, F., Zeigler, M., Wu, C., & Chiu, D. T. (2011) · ACS Nano · doi:10.1021/nn103304m · Cited by 211 · see citing works
Fluorescent Hybridization Probes for Sensitive and Selective DNA and RNA Detection
Martı́, A. A., Jockusch, S., Stevens, N., et al. (2007) · Accounts of Chemical Research · doi:10.1021/ar600013q · Cited by 181 · see citing works
Folic acid-functionalized two-photon absorbing nanoparticles for targeted MCF-7 cancer cell imaging
Li, K., Jiang, Y., Ding, D., Zhang, X., Liu, Y., Hua, J., Feng, S., & Liu, B. (2011) · Chemical Communications · doi:10.1039/c1cc10739a · Cited by 152 · see citing works
Ling, D., Xia, H., Park, W., Hackett, M., Song, C., Na, K., Hui, K. M., & Hyeon, T. (2014) · ACS Nano · doi:10.1021/nn502074x · Cited by 135 · see citing works
High‐Order Non‐Linear Optical Effects in Organic Luminogens with Aggregation‐Induced Emission
Qian, J., Zhu, Z., Qin, A., Qin, W., Chu, L., Cai, F., Zhang, H., Wu, Q., Hu, R., Tang, B. Z., et al. (2015) · Advanced Materials · doi:10.1002/adma.201500141 · Cited by 108 · see citing works
Two-Photon Graphene Oxide/Aptamer Nanosensing Conjugate for In Vitro or In Vivo Molecular Probing
Yi, M., Yang, S., Peng, Z., et al. (2014) · Analytical Chemistry · doi:10.1021/ac5000015 · Cited by 106 · see citing works
Maestro, L. M., Ramírez-Hernández, J. E., Bogdan, N., Capobianco, J. A., Vetrone, F., Solé, J. G., & Jaque, D. (2011) · Nanoscale · doi:10.1039/c1nr11285f · Cited by 97 · see citing works
Lv, P., Ma, Y., Yu, R., et al. (2012) · Molecular Pharmaceutics · doi:10.1021/mp300051h · Cited by 77 · see citing works
Geng, J., Goh, C. C., Tomczak, N., Liu, J., Liu, R., Ma, L., Ng, L. G., Gurzadyan, G. G., & Liu, B. (2014) · Chemistry of Materials · doi:10.1021/cm4040374 · Cited by 71 · see citing works
Stanciu, S. G., Xu, S., Peng, Q., Yan, J., Stanciu, G. A., Welsch, R. E., So, P. T. C., Csúcs, G., & Yu, H. (2014) · Scientific Reports · doi:10.1038/srep04636 · Cited by 66 · see citing works
Zhang, H., Fan, J., Wang, K., et al. (2014) · Analytical Chemistry · doi:10.1021/ac501944y · Cited by 66 · see citing works
Deep tissue fluorescence imaging and in vivo biological applications
Crosignani, V., Dvornikov, A. S., Aguilar, J. S., et al. (2012) · Journal of Biomedical Optics · doi:10.1117/1.jbo.17.11.116023 · Cited by 65 · see citing works
Gu, M., Gan, X. S., Kisteman, A., & Xu, M. G. (2000) · Applied Physics Letters · doi:10.1063/1.1308059 · Cited by 59 · see citing works
Xu, Z., Liao, Q., Wu, Y., Ren, W., Li, W., Liu, L., Wang, S., Gu, Z., Zhang, H., & Fu, H. (2012) · Journal of Materials Chemistry · doi:10.1039/c2jm33081d · Cited by 56 · see citing works
Self-citationLiu, P., Li, S., Jin, Y., Qian, L., Gao, N., Yao, S. Q., Huang, F., Xu, Q., & Cao, Y. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b00223 · Cited by 54 · see citing works
Self-citationLanger, G., Bouchal, K.-D., Grün, H., et al. (2013) · Optics Express · doi:10.1364/oe.21.022410 · Cited by 47 · see citing works
Aparicio‐Ixta, L., Ramos‐Ortíz, G., Pichardo-Molina, J. L., et al. (2012) · Nanoscale · doi:10.1039/c2nr31925j · Cited by 39 · see citing works
Two-photon probes for biomedical applications
Lim, C. S., & Cho, B. R. (2013) · BMB Reports · doi:10.5483/bmbrep.2013.46.4.045 · Cited by 36 · see citing works
Tian, Z., Yu, J., Wang, X., et al. (2012) · The Journal of Physical Chemistry B · doi:10.1021/jp3086254 · Cited by 33 · see citing works
Self-citationEvanko, D. S. (2005) · Nature Methods · doi:10.1038/nmeth1205-901 · Cited by 22 · see citing works
Deep tissue imaging by enhanced photon collection
Crosignani, V., Jahid, S., Dvornikov, A. S., & Gratton, E. (2014) · Journal of Innovative Optical Health Sciences · doi:10.1142/s1793545814500345 · Cited by 7 · see citing works
Biophotonics: Harnessing Light for Biology and Medicine
Ohulchanskyy, T. Y., Pliss, A., & Prasad, P. N. (2010) · NATO science for peace and security series. B, Physics and biophysics · doi:10.1007/978-90-481-9977-8_1 · Cited by 3 · see citing works
Ultra-deep imaging of turbid samples by enhanced photon harvesting
Crosignani, V., Dvornikov, A. S., & Gratton, E. (2013) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.2002101 · Cited by 3 · see citing works
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Piwoński, H., Nozue, S., & Habuchi, S. (2022) · ACS Nanoscience Au · doi:10.1021/acsnanoscienceau.1c00038 · Cited by 39 · see citing works
- Authors
- Lv, Y., Liu, P., Ding, H., Wu, Y., Yan, Y., Liu, H., Wang, X., Huang, F., Zhao, Y., & Tian, Z.
- Affiliations
- University of Chinese Academy of Sciences (CN)
- State Key Laboratory of Luminescent Materials and Devices (CN)
- Chinese Academy of Sciences (CN)
- Beijing National Laboratory for Molecular Science (BNLMS) and Key Laboratory for Photochemistry, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, China
- Corresponding author
- Huang, Fei
- Tian, Zhiyuan
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-09-15
- Volume
- 7
- Issue
- 37
- Pages
- 20640-20648
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2423896238
- Cited by
- 56
- References
- 37
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 3 · 2022: 7 · 2021: 7 · 2020: 3 · 2019: 7 · 2018: 6 · 2017: 12 · 2016: 9
- FWCI
- 3.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Chinese Academy of Sciences
- National Natural Science Foundation of China (21173262, 21373218, 91233107)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
- Physical Sciences › Engineering › Biomedical Engineering › Nonlinear Optical Materials Studies
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Keywords
- conjugated polymers, hybrid nanostructures, two-photon excitation, near-infrared fluorescence, two-photon absorption, fluorescence resonance energy transfer, fluorescence imaging, biological window, deep tissue penetration, HeLa cells
- MeSH terms
- Dynamic Light Scattering, Cell Survival, Diagnostic Imaging, Fluorescent Dyes, HeLa Cells, Humans, Polymers, Spectrometry, Fluorescence, Photons, Spectroscopy, Near-Infrared, Nanoparticles, Absorption, Radiation
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