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Ultrasmall Pt Nanoclusters as Robust Peroxidase Mimics for Colorimetric Detection of Glucose in Human Serum
Abstract
In this work, a new type of ultrasmall Pt nanoclusters (Pt NCs) was prepared via a facile one-pot approach by using yeast extract as the reductant and stabilizer. Besides their excellent water solubility, these yeast extract-stabilized Pt NCs also possess attractive peroxidase mimicking property. They can efficiently catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the coexistence of hydrogen peroxide (H 2 O 2 ). Catalytic mechanism analysis suggested that the peroxidase mimicking activity of these Pt NCs might originate from their characteristic of accelerating electron transfer between TMB and H 2 O 2, and their enzymatic kinetics followed typical Michaelis–Menten theory. On the basis of these findings, we developed a new highly sensitive colorimetric method for glucose detection, and the limit of detection was calculated as low as 0.28 μM (S/N = 3). Further application of the present system for glucose detection in human serum has been successfully demonstrated, suggesting its promising utilization as robust peroxidase mimics in the clinical diagnosis, pharmaceutical, and environmental chemistry fields.
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Metadata
- Authors
- Jin, L., Meng, Z., Zhang, Y., Cai, S., Zhang, Z., Li, C., Shang, L., & Shen, Y.
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-03-08
- Volume
- 9
- Issue
- 11
- Pages
- 10027-10033
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2591961418
- Cited by
- 339
- References
- 45
- FWCI
- 17.50
- Type
- journal article
- Language
- en
- Open access
- closed
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