Cited by 7,483Advanced Nanomaterials in CatalysisEnzyme Catalysis and ImmobilizationCharacterization and Applications of Magnetic Nanoparticles

Intrinsic peroxidase-like activity of ferromagnetic nanoparticles

Gao, Lizeng, Zhuang, Jie, Nie, Leng, Zhang, Jinbin, Zhang, Yu, Gu, Ning, Wang, Taihong, Feng, Jing +3 more·2007·Nature Nanotechnology

Abstract

Nanoparticles containing magnetic materials, such as magnetite (Fe3O4), are particularly useful for imaging and separation techniques. As these nanoparticles are generally considered to be biologically and chemically inert, they are typically coated with metal catalysts, antibodies or enzymes to increase their functionality as separation agents. Here, we report that magnetite nanoparticles in fact possess an intrinsic enzyme mimetic activity similar to that found in natural peroxidases, which are widely used to oxidize organic substrates in the treatment of wastewater or as detection tools. Based on this finding, we have developed a novel immunoassay in which antibody-modified magnetite nanoparticles provide three functions: capture, separation and detection. The stability, ease of production and versatility of these nanoparticles makes them a powerful tool for a wide range of potential applications in medicine, biotechnology and environmental chemistry.

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Crossref · 10.1038/nnano.2007.260

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Authors
Gao, L., Zhuang, J., Nie, L., Zhang, J., Zhang, Y., Gu, N., Wang, T., Feng, J., Yang, D., Perrett, S., & Yan, X.
Affiliations
  • Chinese Academy of Sciences (CN)
  • State Key Laboratory of Digital Medical Engineering (CN)
Corresponding author
  • Perrett, Sarah
  • Yan, Xiyun
Venue
Nature Nanotechnology
Published
2007-08-26
Volume
2
Issue
9
Pages
577-583
ISSN
  • 1748-3387 (ISSN-L)
  • 1748-3395
OpenAlex
W2170913114
Cited by
7,483
References
37
Citations by year
2026: 763 · 2025: 898 · 2024: 877 · 2023: 835 · 2022: 757 · 2021: 650 · 2020: 564 · 2019: 467 · 2018: 348 · 2017: 289 · 2016: 229 · 2015: 197 · 2014: 167 · 2013: 132 · 2012: 118
FWCI
17.69
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Nanotechnology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China
  • Chinese Academy of Sciences
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Advanced Nanomaterials in Catalysis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
  • Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
Keywords
Fe3O4 nanoparticles, peroxidase-like activity, enzyme mimics, immunoassay, magnetic nanoparticles, nanoparticle separation, wastewater treatment, organic substrate oxidation
MeSH terms
Immunoassay, Peroxidase, Biosensing Techniques, Nanotechnology, Biomimetic Materials, Ferrosoferric Oxide

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