Intrinsic peroxidase-like activity of ferromagnetic nanoparticles
Abstract
Nanoparticles containing magnetic materials, such as magnetite (Fe3O4), are particularly useful for imaging and separation techniques. As these nanoparticles are generally considered to be biologically and chemically inert, they are typically coated with metal catalysts, antibodies or enzymes to increase their functionality as separation agents. Here, we report that magnetite nanoparticles in fact possess an intrinsic enzyme mimetic activity similar to that found in natural peroxidases, which are widely used to oxidize organic substrates in the treatment of wastewater or as detection tools. Based on this finding, we have developed a novel immunoassay in which antibody-modified magnetite nanoparticles provide three functions: capture, separation and detection. The stability, ease of production and versatility of these nanoparticles makes them a powerful tool for a wide range of potential applications in medicine, biotechnology and environmental chemistry.
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Acid-activatable metal-based nanozymes (e.g., Fe3O4) exhibit peroxidase-like activity within the acidic (pH 5.5-6.5), H2O2-rich microenvironment of infected bone.
“Among these, acid-activatable metal-based nanozymes (e.g., Fe3O4) hold particular promise, exhibiting peroxidase-like activity within the acidic (pH 5.5–6.5), H2O2-rich microenvironment of infected bone [[12], [13], [14]].”
From Nanocatalytic magnesium osteoimplants with biodegradable self-adaptive interfaces for therapeutic repair of infected bone defects (2026) · cites this paper as [14]
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“Among these, acid-activatable metal-based nanozymes (e.g., Fe 3 O 4 ) hold particular promise, exhibiting peroxidase-like activity within the acidic (pH 5.5 – 6.5), H 2 O 2 -rich microenvironment of infected bone [12 – 14].”
InfluentialSelf-citation
- Authors
- Gao, L., Zhuang, J., Nie, L., Zhang, J., Zhang, Y., Gu, N., Wang, T., Feng, J., Yang, D., Perrett, S., & Yan, X.
- Affiliations
- Chinese Academy of Sciences (CN)
- State Key Laboratory of Digital Medical Engineering (CN)
- Corresponding author
- Perrett, Sarah
- Yan, Xiyun
- Venue
- Nature Nanotechnology
- Published
- 2007-08-26
- Volume
- 2
- Issue
- 9
- Pages
- 577-583
- ISSN
- 1748-3387 (ISSN-L)
- 1748-3395
- OpenAlex
- W2170913114
- Cited by
- 7,483
- References
- 37
- Citations by year
- 2026: 763 · 2025: 898 · 2024: 877 · 2023: 835 · 2022: 757 · 2021: 650 · 2020: 564 · 2019: 467 · 2018: 348 · 2017: 289 · 2016: 229 · 2015: 197 · 2014: 167 · 2013: 132 · 2012: 118
- FWCI
- 17.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Nanotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Chinese Academy of Sciences
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Advanced Nanomaterials in Catalysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Keywords
- Fe3O4 nanoparticles, peroxidase-like activity, enzyme mimics, immunoassay, magnetic nanoparticles, nanoparticle separation, wastewater treatment, organic substrate oxidation
- MeSH terms
- Immunoassay, Peroxidase, Biosensing Techniques, Nanotechnology, Biomimetic Materials, Ferrosoferric Oxide
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