Cited by 63Hydrogels: synthesis, properties, applicationsSupramolecular Self-Assembly in MaterialsAdvanced Materials and Mechanics

Engineering the Dynamic Properties of Protein Networks through Sequence Variation

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Dooling, Lawrence J., Tirrell, David A.·2016·ACS Central Science

Abstract

The dynamic behavior of macromolecular networks dominates the mechanical properties of soft materials and influences biological processes at multiple length scales. In hydrogels prepared from self-assembling artificial proteins, stress relaxation and energy dissipation arise from the transient character of physical network junctions. Here we show that subtle changes in sequence can be used to program the relaxation behavior of end-linked networks of engineered coiled-coil proteins. Single-site substitutions in the coiled-coil domains caused shifts in relaxation time over 5 orders of magnitude as demonstrated by dynamic oscillatory shear rheometry and stress relaxation measurements. Networks with multiple relaxation time scales were also engineered. This work demonstrates how time-dependent mechanical responses of macromolecular materials can be encoded in genetic information.

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  • Recombinant proteins are molecularly well-defined and can be tuned independently for stiffness, viscoelastic behavior, and chemical functionality.

    “Recombinant proteins are molecularly well-defined and can be tuned independently for stiffness, viscoelastic behavior, and chemical functionality203–205.”

    From Towards organoid culture without Matrigel (2021) · cites this paper as [205]

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Authors
Dooling, L. J., & Tirrell, D. A.
Affiliations
California Institute of Technology (US)
Corresponding author
Tirrell, David A.
Venue
ACS Central Science
Published
2016-10-18
Volume
2
Issue
11
Pages
812-819
ISSN
  • 2374-7943 (ISSN-L)
  • 2374-7951
OpenAlex
W2533589637
Cited by
63
References
46
Citations by year
2026: 3 · 2025: 3 · 2024: 9 · 2023: 6 · 2022: 8 · 2021: 5 · 2020: 8 · 2019: 11 · 2018: 7 · 2017: 3
FWCI
3.41
Type
journal article
Language
en
Open access
Locations
  • ACS Central Science (publishedVersion, other-oa)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • CaltechAUTHORS (California Institute of Technology) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Science Foundation (DMR-1506483)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Physical Sciences › Materials Science › Biomaterials › Supramolecular Self-Assembly in Materials
  • Physical Sciences › Engineering › Mechanical Engineering › Advanced Materials and Mechanics
Keywords
coiled-coil proteins, engineered proteins, protein hydrogels, stress relaxation, relaxation time, sequence variation, dynamic mechanical properties, protein self-assembly, soft materials

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