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Cited by 379

The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.

Surface-Adaptive, Antimicrobially Loaded, Micellar Nanocarriers with Enhanced Penetration and Killing Efficiency in Staphylococcal Biofilms

Liu, Yong, Busscher, Henk J., Zhao, Bingran, Li, Yuanfeng, Zhang, Zhenkun, van der Mei, Henny C., Ren, Yijin, Shi, Linqi·2016·ACS Nano
DOI 10.1021/acsnano.6b01370
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Abstract

Biofilms cause persistent bacterial infections and are extremely recalcitrant to antimicrobials, due in part to reduced penetration of antimicrobials into biofilms that allows bacteria residing in the depth of a biofilm to survive antimicrobial treatment. Here, we describe the preparation of surface-adaptive, Triclosan-loaded micellar nanocarriers showing (1) enhanced biofilm penetration and accumulation, (2) electrostatic targeting at acidic pH toward negatively charged bacterial cell surfaces in a biofilm, and (3) antimicrobial release due to degradation of the micelle core by bacterial lipases. First, it was established that mixed-shell-polymeric-micelles (MSPM) consisting of a hydrophilic poly(ethylene glycol) (PEG)-shell and pH-responsive poly(β-amino ester) become positively charged at pH 5.0, while being negatively charged at physiological pH. This is opposite to single-shell-polymeric-micelles (SSPM) possessing only a PEG-shell and remaining negatively charged at pH 5.0. The stealth properties of the PEG-shell combined with its surface-adaptive charge allow MSPMs to penetrate and accumulate in staphylococcal biofilms, as demonstrated for fluorescent Nile red loaded micelles using confocal-laser-scanning-microscopy. SSPMs, not adapting a positive charge at pH 5.0, could not be demonstrated to penetrate and accumulate in a biofilm. Once micellar nanocarriers are bound to a staphylococcal cell surface, bacterial enzymes degrade the MSPM core to release its antimicrobial content and kill bacteria over the depth of a biofilm. This constitutes a highly effective pathway to control blood-accessible staphylococcal biofilms using antimicrobials, bypassing biofilm recalcitrance to antimicrobial penetration.

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Metadata

Authors
Liu, Y., Busscher, H. J., Zhao, B., Li, Y., Zhang, Z., van der Mei, H. C., Ren, Y., & Shi, L.
Venue
ACS Nano
Publisher
American Chemical Society (ACS)
Published
2016-03-24
Volume
10
Issue
4
Pages
4779-4789
ISSN
  • 1936-0851 (ISSN-L)
  • 1936-086X
DOI
10.1021/acsnano.6b01370
OpenAlex
W2309290067
Cited by
379
References
51
FWCI
13.54
Type
journal article
Language
en
Open access
closed

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