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Cited by 25Tissue Engineering and Regenerative MedicineTracheal and airway disorders3D Printing in Biomedical Research

Rapid Preparation Method for Preparing Tracheal Decellularized Scaffolds: Vacuum Assistance and Optimization of DNase I

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Wang, Zhihao, Sun, Fei, Lu, Yi, Zhang, Boyou, Zhang, Guozhong, Shi, Hongcan·2021·ACS Omega
DOI 10.1021/acsomega.0c06247PMC PMC8153783
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Abstract

Decellularized scaffolds are an effective way for tracheal tissue engineering to perform alternative treatments. However, clinically used decellularized tracheal scaffolds have a long preparation cycle. The purpose of this study is to improve the efficiency of decellularization by vacuum assistance and optimizing the concentration of DNase I in the decellularization process and to quickly obtain tracheal decellularized scaffolds. The trachea of New Zealand white rabbits was decellularized with 2, 4, 6, and 8 KU/mL DNase I under vacuum. The performance of the decellularized tracheal scaffold was evaluated through histological analysis, immunohistochemical staining, DNA residue, extracellular matrix composition, scanning electron microscopy, mechanical properties, cell compatibility, and in vivo experiments. Histological analysis and immunohistochemical staining showed that compared with the native trachea, the hierarchical structure of the decellularized trachea remained unchanged after decellularization, nonchondrocytes were effectively removed, and the antigenicity of the scaffold was significantly weakened. Deoxyribonucleic acid (DNA) quantitative analysis showed that the amount of residual DNA in the 6-KU group was significantly decreased. Scanning electron microscopy and mechanical tests showed that small gaps appeared in the basement membrane of the 6-KU group, and the mechanical properties decreased. The CCK-8 test results and in vivo experiments showed that the 6-KU group's acellular scaffold had good cell compatibility and new blood vessels were visible on the surface. Taken together, the 6-KU group could quickly prepare rabbit tracheal scaffolds with good decellularization effects in only 2 days, which significantly shortened the preparation cycle reducing the required cost.

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Cited by

Sorted by citations

Authors
Wang, Z., Sun, F., Lu, Y., Zhang, B., Zhang, G., & Shi, H.
Affiliations
  • Yangzhou University (CN)
  • Department of Thoracic Surgery, School of Clinical Medicine, Yangzhou University, and Center for Translational Medicine Research, Yangzhou University, Yangzhou 225000, China
Corresponding author
Shi, Hongcan
ORCID
Venue
ACS Omega
Publisher
American Chemical Society (ACS)
Published
2021-04-16
Volume
6
Issue
16
Pages
10637-10644
ISSN
2470-1343 (ISSN-L)
DOI
10.1021/acsomega.0c06247
PMC
PMC8153783
OpenAlex
W3156238296
Cited by
25
References
3
Citations by year
2026: 3 · 2025: 1 · 2024: 7 · 2023: 7 · 2022: 6 · 2021: 1
FWCI
4.93
Type
journal article
Language
en
Open access
Licence
Locations
  • ACS Omega (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (81770018, 82070020)
  • Jiangsu Graduate Research Innovation Program (XKYCX19_153)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Tracheal and airway disorders
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
tracheal tissue engineering, extracellular matrix, scaffold mechanical properties, cell compatibility, vascularization, rabbit trachea

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