Cited by 152Nanoparticles: synthesis and applicationsAntimicrobial agents and applicationsNanocomposite Films for Food Packaging

Iodine-Stabilized Cu Nanoparticle Chitosan Composite for Antibacterial Applications

Mallick, Sadhucharan, Sharma, Shilpa, Banerjee, Madhuchanda, Ghosh, Siddhartha Sankar, Chattopadhyay, Arun, Paul, Anumita·2012·ACS Applied Materials & Interfaces

Abstract

We report herein the synthesis of a new composite consisting of Cu nanoparticles (NPs) and chitosan (CS), which has been found to be stable in the presence of molecular iodine and has also high antimicrobial activities. The composite could be obtained when aqueous CuSO(4) was treated with hydrazine in the presence of CS. The spherical Cu NPs present in the composite were of average diameters 8±4 nm. The NPs were unstable in atmospheric conditions leading to the formation of oxides of Cu. On the other hand, when molecular iodine was added to the medium following synthesis the NPs were rather stable. Studies of antibacterial property were carried out on Gram-negative green fluorescent expressing Escherichia coli bacteria & Gram-positive Bacillus cereus bacteria. The minimum inhibitory concentration (MIC) of the iodinated composite on Escherichia coli was found to be 130.8 μg/mL, which contained 21.5 μg/mL Cu NPs. This determined value of MIC for Cu NPs was much lower than those reported in the literature. Zeta potential (ζ) measurements supported an attractive interaction between iodinated CS-Cu NP composite and bacteria which was further supported by electron microscopic images. Electron microscopic and flow cytometric studies revealed that the iodinated CS-Cu NP composite was attached to the bacterial cell wall, which caused irreversible damage to the membrane, eventually leading to cell death. Mechanism of bactericidal action of the iodinated composite is discussed in light of our findings.

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Crossref · 10.1021/am201586w

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  • Chitosan contains a large number of amino and hydroxyl functional groups and possesses excellent adsorption ability for heavy metals, dyestuffs, and pathogens and can be easily functionalized.

    “Chitosan contains a large number of amino (-NH 2 ) and hydroxyl (-OH) functional groups and possesses excellent adsorption ability for a wide variety of environmental pollutants such as heavy metals, 62 dyestuffs, 62 and pathogens 63 and can be easily functionalized.64”

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Authors
Mallick, S., Sharma, S., Banerjee, M., Ghosh, S. S., Chattopadhyay, A., & Paul, A.
Affiliations
Indian Institute of Technology Guwahati (IN)
Corresponding author
  • Chattopadhyay, Arun
  • Paul, Anumita
Venue
ACS Applied Materials & Interfaces
Published
2012-02-16
Volume
4
Issue
3
Pages
1313-1323
ISSN
  • 1944-8244 (ISSN-L)
  • 1944-8252
OpenAlex
W2000166788
Cited by
152
References
50
Citations by year
2026: 5 · 2025: 7 · 2024: 7 · 2023: 16 · 2022: 11 · 2021: 11 · 2020: 10 · 2019: 11 · 2018: 9 · 2017: 9 · 2016: 13 · 2015: 16 · 2014: 11 · 2013: 12 · 2012: 4
FWCI
6.33
Type
journal article
Language
en
Open access
closed
Locations
  • ACS Applied Materials & Interfaces (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Council of Scientific and Industrial Research, India
  • Indian Institute of Technology Guwahati
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
  • Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
  • Physical Sciences › Materials Science › Biomaterials › Nanocomposite Films for Food Packaging
Keywords
copper nanoparticles, chitosan, nanoparticle composites, antibacterial activity, Escherichia coli, Bacillus cereus, minimum inhibitory concentration, zeta potential, bacterial cell membrane damage, hydrazine reduction, nanoparticle stability
MeSH terms
Anti-Bacterial Agents, Cell Membrane, Copper, Escherichia coli, Flow Cytometry, Iodine, Microbial Sensitivity Tests, Powders, Spectrophotometry, Ultraviolet, Time Factors, X-Ray Diffraction, Chitosan, Green Fluorescent Proteins, Nanoparticles

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