Iodine-Stabilized Cu Nanoparticle Chitosan Composite for Antibacterial Applications
Abstract
We report herein the synthesis of a new composite consisting of Cu nanoparticles (NPs) and chitosan (CS), which has been found to be stable in the presence of molecular iodine and has also high antimicrobial activities. The composite could be obtained when aqueous CuSO(4) was treated with hydrazine in the presence of CS. The spherical Cu NPs present in the composite were of average diameters 8±4 nm. The NPs were unstable in atmospheric conditions leading to the formation of oxides of Cu. On the other hand, when molecular iodine was added to the medium following synthesis the NPs were rather stable. Studies of antibacterial property were carried out on Gram-negative green fluorescent expressing Escherichia coli bacteria & Gram-positive Bacillus cereus bacteria. The minimum inhibitory concentration (MIC) of the iodinated composite on Escherichia coli was found to be 130.8 μg/mL, which contained 21.5 μg/mL Cu NPs. This determined value of MIC for Cu NPs was much lower than those reported in the literature. Zeta potential (ζ) measurements supported an attractive interaction between iodinated CS-Cu NP composite and bacteria which was further supported by electron microscopic images. Electron microscopic and flow cytometric studies revealed that the iodinated CS-Cu NP composite was attached to the bacterial cell wall, which caused irreversible damage to the membrane, eventually leading to cell death. Mechanism of bactericidal action of the iodinated composite is discussed in light of our findings.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Chitosan contains a large number of amino and hydroxyl functional groups and possesses excellent adsorption ability for heavy metals, dyestuffs, and pathogens and can be easily functionalized.
“Chitosan contains a large number of amino (-NH 2 ) and hydroxyl (-OH) functional groups and possesses excellent adsorption ability for a wide variety of environmental pollutants such as heavy metals, 62 dyestuffs, 62 and pathogens 63 and can be easily functionalized.64”
From Sustainable hybrid photocatalysts: titania immobilized on carbon materials derived from renewable and biodegradable resources (2016) · cites this paper as [63]
Cites
Jain, P. K., Huang, X., El‐Sayed, I. H., & El‐Sayed, M. A. (2008) · Accounts of Chemical Research · doi:10.1021/ar7002804 · Cited by 4,235 · see citing works
Chitosan as antimicrobial agent: applications and mode of action.
Rabea, E. I., Badawy, M. E.-T., Stevens, C. V., Smagghe, G., & Steurbaut, W. (2003) · Biomacromolecules · doi:10.1021/bm034130m · Cited by 3,123 · see citing works
Disinfection, Sterilization, and Preservation
Block, S. S. (1977) · Soil Science · doi:10.1097/00010694-197712000-00013 · Cited by 1,963 · see citing works
Strain specificity in antimicrobial activity of silver and copper nanoparticles
Ruparelia, J. P., Chatterjee, A. K., Duttagupta, S. P., & Mukherji, S. (2007) · Acta Biomaterialia · doi:10.1016/j.actbio.2007.11.006 · Cited by 1,888 · see citing works
Gaggelli, E., Kozlowski, H., Valensin, D., & Valensin, G. (2006) · Chemical Reviews · doi:10.1021/cr040410w · Cited by 1,729 · see citing works
Ultraviolet–visible absorption spectra of the colloidal metallic elements
Creighton, J. A., & Eadon, D. G. (1991) · J. Chem. Soc., Faraday Trans. · doi:10.1039/ft9918703881 · Cited by 1,625 · see citing works
Huang, X., Jain, P. K., El‐Sayed, I. H., & El‐Sayed, M. A. (2007) · Nanomedicine · doi:10.2217/17435889.2.5.681 · Cited by 1,510 · see citing works
Chitosan disrupts the barrier properties of the outer membrane of Gram-negative bacteria
Helander, I. M., Nurmiaho‐Lassila, E., Ahvenainen, R. T., Rhoades, J., & Roller, S. D. (2001) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(01)00609-2 · Cited by 1,368 · see citing works
Atom-Resolved Imaging of Dynamic Shape Changes in Supported Copper Nanocrystals
Hansen, P. L., Wagner, J. B., Helveg, S., Rostrup-Nielsen, J. R., Clausen, B. S., & Topsøe, H. (2002) · Science · doi:10.1126/science.1069325 · Cited by 1,261 · see citing works
Antibacterial action of chitosan
Sudarshan, N. R., Hoover, D., & Knorr, D. (1992) · Food Biotechnology · doi:10.1080/08905439209549838 · Cited by 1,001 · see citing works
Potential of silver nanoparticle‐coated polyurethane foam as an antibacterial water filter
Jain, P., & Pradeep, T. (2005) · Biotechnology and Bioengineering · doi:10.1002/bit.20368 · Cited by 940 · see citing works
Yoon, K.-Y., Byeon, J. H., Park, J.-H., et al. (2006) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2006.11.007 · Cited by 884 · see citing works
Copper Nanoparticle/Polymer Composites with Antifungal and Bacteriostatic Properties
Cioffi, N., Torsi, L., Ditaranto, N., Tantillo, G. M., Ghibelli, L., Sabbatini, L., Bleve‐Zacheo, T., D'ALESSIO, M., Zambonin, P. G., & Traversa, E. (2005) · Chemistry of Materials · doi:10.1021/cm0505244 · Cited by 834 · see citing works
Borkow, G., & Gabbay, J. (2005) · Current Medicinal Chemistry · doi:10.2174/0929867054637617 · Cited by 727 · see citing works
Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli
Raffi, M., Mehrwan, S., Bhatti, T. M., et al. (2010) · Annals of Microbiology · doi:10.1007/s13213-010-0015-6 · Cited by 689 · see citing works
Synthesis, Characterization, and Properties of Metallic Copper Nanoparticles
Dhas, N. A., Raj, C. P., & Gedanken, A. (1998) · Chemistry of Materials · doi:10.1021/cm9708269 · Cited by 654 · see citing works
Wilson, W. W., Wade, M. M., Holman, S. C., & Champlin, F. R. (2001) · Journal of Microbiological Methods · doi:10.1016/s0167-7012(00)00224-4 · Cited by 629 · see citing works
Antibacterial activity of chitosan tripolyphosphate nanoparticles loaded with various metal ions
Du, W., Niu, S.-S., Xu, Y., Xu, Z., & Fan, C.-L. (2008) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2008.07.039 · Cited by 575 · see citing works
Synthesis of copper metallic clusters using reverse micelles as microreactors
Lisiecki, I., & Pileni, M. P. (1993) · Journal of the American Chemical Society · doi:10.1021/ja00063a006 · Cited by 573 · see citing works
Gogoi, S. K., Gopinath, P., Paul, A., Ramesh, A., Ghosh, S. S., & Chattopadhyay, A. (2006) · Langmuir · doi:10.1021/la060661v · Cited by 446 · see citing works
Self-citationThe antibacterial properties of a novel chitosan–Ag-nanoparticle composite
Sanpui, P., Murugadoss, A., Prasad, P. V. D., Ghosh, S. S., & Chattopadhyay, A. (2008) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2008.03.004 · Cited by 397 · see citing works
Self-citationControl of the Shape and the Size of Copper Metallic Particles
Lisiecki, I., Billoudet, F., & Pileni, M. P. (1996) · The Journal of Physical Chemistry · doi:10.1021/jp9523837 · Cited by 358 · see citing works
Synthesis, Characterization, and Nonlinear Optical Properties of Copper Nanoparticles
Huang, H., Yan, F. Q., Kek, Y. M., Chew, C. H., Xu, G. Q., Ji, W., Oh, P., & Tang, S. H. (1997) · Langmuir · doi:10.1021/la9605495 · Cited by 336 · see citing works
A Novel Method for Preparing Copper Nanorods and Nanowires
Liu, Z., & Bando, Y. (2003) · Advanced Materials · doi:10.1002/adma.200390073 · Cited by 325 · see citing works
Formation and Characteristics of Cu Colloids from CuO Powder by Laser Irradiation in 2-Propanol
Yeh, M.-S., Yang, Y.-S., Lee, Y.-P., Lee, H.-F., Yeh, Y.-H., & Yeh, C. (1999) · The Journal of Physical Chemistry B · doi:10.1021/jp984163+ · Cited by 324 · see citing works
Murugadoss, A., & Chattopadhyay, A. (2007) · Nanotechnology · doi:10.1088/0957-4484/19/01/015603 · Cited by 309 · see citing works
Self-citationSonochemical synthesis of amorphous Cu and nanocrystalline Cu2O embedded in a polyaniline matrix
Kumar, R. V., Mastai, Y., Diamant, Y., & Gedanken, A. (2001) · Journal of Materials Chemistry · doi:10.1039/b005769j · Cited by 292 · see citing works
Chitosan-based heterogeneous catalysts for Suzuki and Heck reactions
Hardy, J. J. E., Hubert, S., Macquarrie, D. J., et al. (2004) · Green Chemistry · doi:10.1039/b312145n · Cited by 258 · see citing works
Silver Products for Medical Indications: Risk-Benefit Assessment
Fung, M. C., & Bowen, D. L. (1996) · Journal of Toxicology Clinical Toxicology · doi:10.3109/15563659609020246 · Cited by 253 · see citing works
Kim, Y. H., Lee, D. K., Cha, H. G., et al. (2006) · The Journal of Physical Chemistry B · doi:10.1021/jp0656779 · Cited by 252 · see citing works
Radiation induced synthesis and characterization of copper nanoparticles
Joshi, S. S., Patil, S. F., Iyer, V. S., et al. (1998) · Nanostructured Materials · doi:10.1016/s0965-9773(98)00153-6 · Cited by 233 · see citing works
Antibacterial activity of copper monodispersed nanoparticles into sepiolite
Esteban-Cubillo, A., Pecharromán, C., Aguilar, E., et al. (2006) · Journal of Materials Science · doi:10.1007/s10853-006-0432-x · Cited by 226 · see citing works
Synthesis of Copper Nanoparticles in Nonionic Water-in-Oil Microemulsions
Qi, L., Ma, J., & Shen, J. (1997) · Journal of Colloid and Interface Science · doi:10.1006/jcis.1996.4647 · Cited by 209 · see citing works
Synthesis, Characterization, and Antifouling Potential of Functionalized Copper Nanoparticles
Anyaogu, K. C., Fedorov, A. V., & Neckers, D. C. (2008) · Langmuir · doi:10.1021/la800102f · Cited by 201 · see citing works
Banerjee, M., Mallick, S., Paul, A., et al. (2010) · Langmuir · doi:10.1021/la9038528 · Cited by 197 · see citing works
Self-citationLi, J., & McLandsborough, L. (1999) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(99)00159-2 · Cited by 189 · see citing works
Wei, Y., Chen, S., Kowalczyk, B., Huda, S., Gray, T. P., & Grzybowski, B. A. (2010) · The Journal of Physical Chemistry C · doi:10.1021/jp1055683 · Cited by 169 · see citing works
Lyday, P. A. (2000) · Ullmann's Encyclopedia of Industrial Chemistry · doi:10.1002/14356007.a14_381 · Cited by 168 · see citing works
Nanoscale Copper Particles Derived from Solvated Cu Atoms in the Activation of Molecular Oxygen
Vitulli, G., Bernini, M., Bertozzi, S., Pitzalis, E., Salvadori, P. A., Coluccia, S., & Martra, G. (2002) · Chemistry of Materials · doi:10.1021/cm011199x · Cited by 167 · see citing works
Synthesis, characterization and antibacterial investigation of silver–copper nanoalloys
Camcı, M. T., Sayar, N., Yuluğ, I. G., & Süzer, Ş. (2011) · Journal of Materials Chemistry · doi:10.1039/c1jm11718a · Cited by 140 · see citing works
ATP7B Mediates Vesicular Sequestration of Copper: Insight Into Biliary Copper Excretion
Cater, M. A., Fontaine, S. L., Shield, K., Deal, Y., & Mercer, J. F. B. (2006) · Gastroenterology · doi:10.1053/j.gastro.2005.10.054 · Cited by 137 · see citing works
The Microstructure of Copper Zinc Oxide Catalysts: Bridging the Materials Gap
Ressler, T., Kniep, B. L., Kasatkin, I., & Schlögl, R. (2005) · Angewandte Chemie International Edition · doi:10.1002/anie.200462942 · Cited by 131 · see citing works
Carbon-halogen bond dissociation on copper surfaces: effect of alkyl chain length
Lin, J. L., & Bent, B. E. (1992) · The Journal of Physical Chemistry · doi:10.1021/j100200a059 · Cited by 128 · see citing works
Quasi‐Homogeneous Methanol Synthesis Over Highly Active Copper Nanoparticles
Vukojević, S., Trapp, O., Grunwaldt, J., et al. (2005) · Angewandte Chemie International Edition · doi:10.1002/anie.200503169 · Cited by 124 · see citing works
Casella, I. G., Cataldi, T. R. I., Guerrieri, A., et al. (1996) · Analytica Chimica Acta · doi:10.1016/s0003-2670(96)00351-0 · Cited by 111 · see citing works
Du, W., Xu, Y., Xu, Z., et al. (2008) · Nanotechnology · doi:10.1088/0957-4484/19/8/085707 · Cited by 105 · see citing works
Influence of I− anions on the formation and stabilization of copper nanoparticles
Kapoor, S., Joshi, R. K., & Mukherjee, T. (2002) · Chemical Physics Letters · doi:10.1016/s0009-2614(02)00159-8 · Cited by 56 · see citing works
Andryushechkin, B. V., Eltsov, K. N., & Shevlyuga, V. M. (2004) · Surface Science · doi:10.1016/j.susc.2004.05.045 · Cited by 26 · see citing works
Jenks, C. J., Paul, A., Smoliar, L. A., et al. (1994) · The Journal of Physical Chemistry · doi:10.1021/j100053a036 · Cited by 25 · see citing works
Self-citationRehse, K., Scheffler, H., & Reitner, N. (1999) · Archiv der Pharmazie · doi:10.1002/(sici)1521-4184(19995)332:5 · Cited by 16 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Colmenares, J. C., Varma, R. S., & Lisowski, P. (2016) · Green Chemistry · doi:10.1039/c6gc02477g · Cited by 197 · see citing works
Synthesis of core–shell bioaffinity chitosan–TiO2 composite and its environmental applications
Xiao, G., Su, H., & Tan, T. (2015) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2014.10.047 · Cited by 85 · see citing works
- Authors
- Mallick, S., Sharma, S., Banerjee, M., Ghosh, S. S., Chattopadhyay, A., & Paul, A.
- Affiliations
- Indian Institute of Technology Guwahati (IN)
- Corresponding author
- Chattopadhyay, Arun
- Paul, Anumita
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-02-16
- Volume
- 4
- Issue
- 3
- Pages
- 1313-1323
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2000166788
- Cited by
- 152
- References
- 50
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 7 · 2023: 16 · 2022: 11 · 2021: 11 · 2020: 10 · 2019: 11 · 2018: 9 · 2017: 9 · 2016: 13 · 2015: 16 · 2014: 11 · 2013: 12 · 2012: 4
- FWCI
- 6.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Council of Scientific and Industrial Research, India
- Indian Institute of Technology Guwahati
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
- Physical Sciences › Materials Science › Biomaterials › Nanocomposite Films for Food Packaging
- Keywords
- copper nanoparticles, chitosan, nanoparticle composites, antibacterial activity, Escherichia coli, Bacillus cereus, minimum inhibitory concentration, zeta potential, bacterial cell membrane damage, hydrazine reduction, nanoparticle stability
- MeSH terms
- Anti-Bacterial Agents, Cell Membrane, Copper, Escherichia coli, Flow Cytometry, Iodine, Microbial Sensitivity Tests, Powders, Spectrophotometry, Ultraviolet, Time Factors, X-Ray Diffraction, Chitosan, Green Fluorescent Proteins, Nanoparticles
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review