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Surface Modification To Reduce Nonspecific Binding of Quantum Dots in Live Cell Assays
Abstract
Nonspecific binding is a frequently encountered problem with fluorescent labeling of tissue cultures when labeled with quantum dots. In these studies various cell lines were examined for nonspecific binding. Evidence suggests that nonspecific binding is related to cell type and may be significantly reduced by functionalizing quantum dots with poly(ethylene glycol) ligands (PEG). The length of PEG required to give a significant reduction in nonspecific binding may be as short as 12-14 ethylene glycol units.
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Dabbousi, B. O., Rodriguez-Viejo, J., Mikulec, F. V., Heine, J. R., Mattoussi, H., Ober, R., Jensen, K. F., & Bawendi, M. G. (1997) · The Journal of Physical Chemistry B · doi:10.1021/jp971091y · Cited by 4,229
Perspectives on the Physical Chemistry of Semiconductor Nanocrystals
Alivisatos, A. P. (1996) · The Journal of Physical Chemistry · doi:10.1021/jp9535506 · Cited by 3,595
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Application of semiconductor quantum dots in bioimaging and biosensing
Martynenko, I. V., Litvin, A. P., Purcell-Milton, F., Baranov, A. V., Fedorov, A. V., & Gun'ko, Y. K. (2017) · Journal of Materials Chemistry B · doi:10.1039/c7tb01425b · Cited by 382
Liu, W., Greytak, A. B., Lee, J., Wong, C. R., Park, J., Marshall, L. F., Jiang, W., Curtin, P. N., Ting, A. Y., Nocera, D. G., et al. (2010) · Journal of the American Chemical Society · doi:10.1021/ja908137d · Cited by 278
Biological interactions of quantum dot nanoparticles in skin and in human epidermal keratinocytes
ZHANG, L., YU, W., COLVIN, V, & MONTEIRORIVIERE, N. (2008) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2007.12.022 · Cited by 247
Metadata
- Authors
- Bentzen, E. L., Tomlinson, I. D., Mason, J., Gresch, P., Warnement, M. R., Wright, D., Sanders-Bush, E., Blakely, R., & Rosenthal, S. J.
- Venue
- Bioconjugate Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-11-01
- Volume
- 16
- Issue
- 6
- Pages
- 1488-1494
- ISSN
- 1043-1802 (ISSN-L)
- 1520-4812
- OpenAlex
- W2069311839
- Cited by
- 277
- References
- 2
- FWCI
- 9.47
- Type
- journal article
- Language
- en
- Open access
- closed
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- Bentzen, E. L., Tomlinson, I. D., Mason, J., Gresch, P., Warnement, M. R., Wright, D., Sanders-Bush, E., Blakely, R., & Rosenthal, S. J. (2005). Surface Modification To Reduce Nonspecific Binding of Quantum Dots in Live Cell Assays. Bioconjugate Chemistry. https://doi.org/10.1021/bc0502006
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