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Continuous Analysis of the Mechanism of Activated Transbilayer Lipid Movement in Platelets
Abstract
Dithionite reduction of fluorescent (NBD) phospholipids was used as the basis of a continuous assay of transbilayer lipid movement to the cell surface during platelet activation. This assay reveals that virtually all previously internalized phosphatidylserine passes through the external leaflet of the membrane within 90 s after activation with Ca2+ and ionophore or with thrombin and thapsigargin. We demonstrate that this lipid scrambling is reversible, bidirectional, and insensitive to the lipid headgroup. Prolonged activation gradually results in inactivation of the scramblase. The assay also reveals that activation of the scrambling activity is sensitive to the sulfhydryl reagent pyridyldithioethylamine, suggesting the involvement of a protein in the process of activated transbilayer lipid scrambling.
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Citations
Cited by
Pathophysiologic Implications of Membrane Phospholipid Asymmetry in Blood Cells
Zwaal, R. F. A., & Schroit, A. J. (1997) · Blood · doi:10.1182/blood.v89.4.1121 · Cited by 1,340
Self-citationThe Distribution and Function of Phosphatidylserine in Cellular Membranes
Leventis, P. A., & Grinstein, S. (2010) · Annual Review of Biophysics · doi:10.1146/annurev.biophys.093008.131234 · Cited by 988
Formation and function of phosphatidylserine and phosphatidylethanolamine in mammalian cells
Vance, J. E., & Tasseva, G. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2012.08.016 · Cited by 521
The Role of Calcium in the Regulation of Apoptosis
McConkey, D. J., & Orrenius, S. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.7409 · Cited by 462
Getting to the Outer Leaflet: Physiology of Phosphatidylserine Exposure at the Plasma Membrane
Bevers, E. M., & Williamson, P. L. (2016) · Physiological Reviews · doi:10.1152/physrev.00020.2015 · Cited by 447
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Metadata
- Authors
- Williamson, P., Bevers, E. M., Smeets, E. F., Comfurius, P., Schlegel, R. A., & Zwaal, R. F. A.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1995-08-22
- Volume
- 34
- Issue
- 33
- Pages
- 10448-10455
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W1981566975
- Cited by
- 174
- References
- 0
- FWCI
- 3.91
- Type
- journal article
- Language
- en
- Open access
- closed
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- Williamson, P., Bevers, E. M., Smeets, E. F., Comfurius, P., Schlegel, R. A., & Zwaal, R. F. A. (1995). Continuous Analysis of the Mechanism of Activated Transbilayer Lipid Movement in Platelets. Biochemistry. https://doi.org/10.1021/bi00033a017
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
