The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
DNA Replication Fidelity with 8-Oxodeoxyguanosine Triphosphate
Abstract
Oxidative metabolism is known to generate mutagenic compounds within cells, among which is 8-oxodeoxyguanosine. Here the mutagenic potential of the triphosphate form of this base analog (8-O-dGTP) is investigated during replication in vitro of the lacZ alpha-complementation sequence in M13mp2 DNA. Adding 8-O-dGTP at equimolar concentration with the normal dNTPs to polymerization reactions decreases the fidelity of DNA synthesis by exonuclease-deficient Klenow, T4, and Thermus thermophilus DNA polymerases. Sequence analysis of mutants suggests that 8-O-dGMP is misincorporated opposite template adenines, yielding A-->C transversions. The degree of polymerase selectivity against this error is enzyme-dependent, with rates varying by > 25-fold. To determine if the A.8-O-dGMP mispair is proofread, a direct comparison of the fidelity of proofreading-proficient and proofreading-deficient Klenow and T4 DNA polymerases was made. Although the exonuclease activity of Klenow polymerase did not substantially reduce overall misincorporation of 8-O-dGMP, misincorporation was lower for the proofreading-proficient T4 enzyme as compared to its proofreading-deficient derivative. These data suggest that the A.8-O-dGMP mispair can be proofread. The mutagenic potential of 8-O-dGTP with eukaryotic systems was also examined. Misincorporation of 8-O-dGTP opposite adenine was observed during SV40 origin-dependent replication of double-stranded DNA in HeLa cell extracts. When present during replication at a concentration equal to the four normal dNTPs, 8-O-dGTP was at least 13-fold more mutagenic for A.T-->C.G transversions than was a 100-fold excess of normal dGTP.(ABSTRACT TRUNCATED AT 250 WORDS)
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“MtDNA oxidation, in turn, renders mtDNA more susceptible to mutation because oxidized bases are misread during replication and this leads to nucleotide substitutions [118–120].”
From Brain aging, Alzheimer's disease, and mitochondria (2011) · cites this paper as [120]
Citations
Cites
Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG
Shibutani, S., Takeshita, M., & Grollman, A. P. (1991) · Nature · doi:10.1038/349431a0 · Cited by 2,224
8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G-T and A-C substitutions.
Cheng, K. C., Cahill, D. S., Kasai, H., Nishimura, S., & Loeb, L. A. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)48474-8 · Cited by 1,844
Mitochondrial Genetics: A Paradigm for Aging and Degenerative Diseases?
Wallace, D. C. (1992) · Science · doi:10.1126/science.1533953 · Cited by 1,262
Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents
Kasai, H., & Nishimura, S. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.4.2137 · Cited by 952
MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis
Maki, H., & Sekiguchi, M. (1992) · Nature · doi:10.1038/355273a0 · Cited by 901
FIDELITY MECHANISMS IN DNA REPLICATION
Echols, H., & Goodman, M. F. (1991) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.60.070191.002401 · Cited by 712
Michaels, M. L., & Miller, J. H. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.20.6321-6325.1992 · Cited by 697
8-oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity.
Tchou, J. C., Kasai, H., Shibutani, S., Chung, M., Laval, J., Grollman, A. P., & Nishimura, S. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.11.4690 · Cited by 689
Wood, M. L., Dizdaroğlu, M., Gajewski, E., & Essigmann, J. M. (1990) · Biochemistry · doi:10.1021/bi00482a011 · Cited by 684
Michaels, M. L., Cruz, C., Grollman, A. P., & Miller, J. H. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.15.7022 · Cited by 598
Cited by
Sorted by citations
Items 101–133 of 133
Accumulation of Adenine DNA Glycosylase-sensitive Sites in Human Mitochondrial DNA
Miyako, K., Takamatsu, C., Umeda, S., Tajiri, T., Furuichi, M., Nakabeppu, Y., Sekiguchi, M., Hamasaki, N., Takeshige, K., & Kang, D. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.16.12326 · Cited by 13
Inhibitory effect of ethyl oleate hydroperoxide and alcohol in photosensitized oxidative DNA damage
Adam, W., Andler, S., Saha‐Möller, C. R., & Schönberger, A. (1996) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/1011-1344(95)07235-7 · Cited by 12
Specialized mismatch repair function of Glu339 in the Phe-X-Glu motif of yeast Msh6
Holmes, S. F., Scarpinato, K. D., McCulloch, S. D., Schaaper, R. M., & Kunkel, T. A. (2006) · DNA repair · doi:10.1016/j.dnarep.2006.10.023 · Cited by 12
Self-citationGoll, J. G., & Thorp, H. H. (1996) · Inorganica Chimica Acta · doi:10.1016/0020-1693(95)04870-7 · Cited by 11
Kasprzak, K. S., Nakabeppu, Y., Kakuma, T., Sakai, Y., Tsuruya, K., Sekiguchi, M., Ward, J. M., Diwan, B. A., Nagashima, K., & Kasprzak, B. H. (2001) · Experimental and Toxicologic Pathology · doi:10.1078/0940-2993-00201 · Cited by 10
Kai, T. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.2.569 · Cited by 9
Synthesis and Incorporation of Pyrrole Carboxamide Nucleoside Triphosphates by DNA Polymerases
Loakes, D., Guo, M., Brown, D. M., Salisbury, S. A., Smith, C. L., Felix, I. R., Kumar, S., & Nampalli, S. (2000) · Nucleosides Nucleotides & Nucleic Acids · doi:10.1080/15257770008045449 · Cited by 9
Wójcik, A., Grzesiuk, E., Tudek, B., & Janion, C. (1996) · Biochimie · doi:10.1016/0300-9084(96)82637-x · Cited by 9
Huang, J. W., Yennie, C. J., & Delaney, S. (2015) · Chemical Research in Toxicology · doi:10.1021/acs.chemrestox.5b00330 · Cited by 8
Białkowski, K., & Kasprzak, K. S. (2004) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2004.07.018 · Cited by 8
Base excision repair enzyme endonuclease III suppresses mutagenesis caused by 8-hydroxy-dGTP
Suzuki, T., Yamamoto, K., Harashima, H., & Kamiya, H. (2007) · DNA repair · doi:10.1016/j.dnarep.2007.08.003 · Cited by 8
Mourgues, S., Trzcionka, J., Vasseur, J., Pratviel, G., & Meunier, B. (2008) · Biochemistry · doi:10.1021/bi7022199 · Cited by 7
Comparison of mutation spectra induced by gamma-rays and carbon ion beams
Tokuyama, Y., Mori, K., Isobe, M., & Terato, H. (2024) · Journal of Radiation Research · doi:10.1093/jrr/rrae050 · Cited by 6
Iksat, N. N., Madirov, A., Artykbayeva, D., Shevchenko, O., Zhanassova, K., Baikarayev, Z., & Masalimov, Z. K. (2025) · Viruses · doi:10.3390/v17091250 · Cited by 6
Oxidative DNA Damage Induced by Dioxetanes, Photosensitizing Ketones, and Photo-Fenton Reagents
Adam, W., Andler, S., Ballmaier, D., Emmert, S., Epe, B., Grimm, G. N., Mielke, K., Møller, M. R., Rünger, T. M., Saha‐Möller, C. R., et al. (1997) · Recent results in cancer research/Recent Results in Cancer Research · doi:10.1007/978-3-642-60393-8_2 · Cited by 6
Processing of DNA single-strand breaks with oxidatively damaged ends by LIG1
Balu, K. E., Almohdar, D., Lerner, C., Ratcliffe, J., Tang, Q., Parwal, T., Lee, K. M., Prakash, A., & Çağlayan, M. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf1344 · Cited by 5
Novel molecular targets for risk identification: DNA repair enzyme activities
Paz-Elizur, T., Elinger, D., Blumenstein, S., Krupsky, M., Schechtman, E., & Livneh, Z. (2007) · Cancer Biomarkers · doi:10.3233/cbm-2007-3303 · Cited by 5
Studies on Oxidative Mechanisms of Metal-Induced Carcinogenesis
Kasprzak, K. S., Bal, W., Porter, D. W., & Białkowski, K. (1999) · Springer eBooks · doi:10.1007/978-1-4615-4865-2_16 · Cited by 5
Mitochondrial DNA Damage and Repair
Shokolenko, I. N., LeDoux, S. P., & Wilson, G. L. (2007) · Springer eBooks · doi:10.1007/978-0-387-69945-5_15 · Cited by 5
Buchko, G. W., Ni, S., Holbrook, S. R., & Kennedy, M. A. (2004) · Proteins Structure Function and Bioinformatics · doi:10.1002/prot.20082 · Cited by 6
Polymerase Synthesis of Base-Modified DNA
Daďová, J., Cahová, H., & Hocek, M. (2016) · Nucleic acids and molecular biology · doi:10.1007/978-3-319-27111-8_6 · Cited by 3
Bushnell, E. A. C., Llano, J., Leif, A., & James, W. (2011) · InTech eBooks · doi:10.5772/22239 · Cited by 2
Hypokinesia Causes Oxidative Damage and Mitochondrial DNA Deletion in Rat Skeletal Muscle.
SAKAI, Y., Iwamura, Y., Nagata, H., Yamamoto, N., Ohkoshi, N., & Ueda, S. (1997) · Journal of Physical Therapy Science · doi:10.1589/jpts.9.103 · Cited by 3
Recognition and Excision of Bases From Oxidatively Damaged DNA by Fpg, Oggl, and MutY Proteins
Grollman, A. P., & Zharkov, D. O. (1999) · Springer eBooks · doi:10.1007/978-1-4615-4865-2_12 · Cited by 3
Base Excision Repair of DNA: Glycosylases
Королев, В. Г. (2005) · Russian Journal of Genetics · doi:10.1007/s11177-005-0131-8 · Cited by 2
Friedberg, E. C., Walker, G. C., Siede, W., Wood, R. D., Schultz, R. A., & Ellenberger, T. E. (2014) · ASM Press eBooks · doi:10.1128/9781555816704.ch2 · Cited by 1
Oxidative Damage of Genome DNA: Repair Mechanisms
Stavridis, J. C. (2007) · Springer eBooks · doi:10.1007/978-1-4020-6704-4_3
Roberts, J. D., & Kunke, T. A. (1996)
THEORETICAL AND EXPERIMENTAL STUDY OF MUTATIONS INDUCED BY 8-OXOGUANINE
Vasyunina, E. A., & Ras, S. (2004)
A Comparative Study for the Incorporation of 8-oxo-dATP in DNA by Human DNA Polymerases
Kruchinin, A. A., Kamzeeva, P. N., Baranov, M. S., Belova, Y. G., Boldinova, E. O., Baranovskiy, A. G., Tahirov, T. H., Aralov, A. V., & Makarova, А. V. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27062537
Fidelity of DNA ligase I is sensitive to physiological Mg2+ level
Beier, D. H., Lee, E., Seong, I. S., Wheeler, V. C., & O’Brien, P. (2026) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2026.111407
Ciblage thérapeutique de la réponse au stress cellulaire dans l'ostéosarcome
Moukengué, B. (2019) · theses.fr (ABES) · doi:10.70675/26cb2736z4fbdz42d2zabcbzca5db0a782e1
Metadata
- Authors
- Pavlov, Y. I., Minnick, D. T., Izuta, S., & Kunkel, T. A.
- Affiliations
- National Institutes of Health (US)
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1994-04-19
- Volume
- 33
- Issue
- 15
- Pages
- 4695-4701
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W1965072222
- Cited by
- 132
- References
- 43
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 5 · 2015: 1 · 2014: 1 · 2013: 1
- FWCI
- 3.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- 8-hydroxy-2'-deoxyguanosine, DNA replication fidelity, oxidative DNA damage, mutagenesis, misincorporation, DNA polymerase proofreading, exonuclease activity, Klenow fragment, T4 DNA polymerase, HeLa cell extracts, base analogs
- MeSH terms
- Base Composition, Deoxyguanine Nucleotides, DNA, DNA Polymerase I, DNA-Directed DNA Polymerase, DNA Replication, Escherichia coli, Glycosylation, Transfection, Mutagenesis, Thermus thermophilus, Bacteriophage T4
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
