The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Evidence for the role of solvated metal hydroxide in the hammerhead cleavage mechanism
Abstract
The log of the rate of the chemical step of hammerhead cleavage in Mg2+ increases linearly with pH between pH 5.7 and 8.9. A slope of approximately 1 indicates that a single deprotonation is required for cleavage. Hammerhead pH-rate profiles with Ca2+, Mn2+, Co2+, and Cd2+ correlate well with the pKa's of these ions in water. This relationship between the pKa's and the pH-rate profile suggests that a metal hydroxide bound to the hammerhead RNA acts as the base in the cleavage mechanism.
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Cites
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Self-citationAutolytic Processing of Dimeric Plant Virus Satellite RNA
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Self-citationSequence requirements of the hammerhead RNA self-cleavage reaction
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Cited by
A general purpose RNA-cleaving DNA enzyme
Santoro, S. W., & Joyce, G. F. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.9.4262 · Cited by 1,541
Three-dimensional structure of a hammerhead ribozyme
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A DNA enzyme with Mg2+-dependent RNA phosphoesterase activity
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General Acid-Base Catalysis in the Mechanism of a Hepatitis Delta Virus Ribozyme
Nakano, S.-I., Chadalavada, D. M., & Bevilacqua, P. C. (2000) · Science · doi:10.1126/science.287.5457.1493 · Cited by 385
Metadata
- Authors
- Dahm, S. C., Derrick, W. B., & Uhlenbeck, O. C.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1993-12-01
- Volume
- 32
- Issue
- 48
- Pages
- 13040-13045
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W1986764455
- Cited by
- 270
- References
- 41
- FWCI
- 6.99
- Type
- journal article
- Language
- en
- Open access
- closed
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- Dahm, S. C., Derrick, W. B., & Uhlenbeck, O. C. (1993). Evidence for the role of solvated metal hydroxide in the hammerhead cleavage mechanism. Biochemistry. https://doi.org/10.1021/bi00211a013
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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