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Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid
Open accessAbstract
Self-cleavage of both plus and minus RNA transcripts of the 247-residue avocado sunblotch viroid (ASBV), prepared from tandem dimeric cDNA clones, occurs specifically at two sites in each transcript to give monomeric plus and minus species. The cleavage reaction occurs both during transcription and on incubation of purified transcripts at pH 8 and 37 degrees C in the presence of magnesium ions to give a 3'-terminal 2',3'-cyclic phosphate and a 5'-terminal hydroxyl group. Although the self-cleavage occurs at different sites in the ASBV molecule for the plus and minus species, very similar secondary structures with high sequence homology can be drawn at each site. The results are considered to provide further evidence that ASBV is replicated in vivo by a rolling circle mechanism involving non-enzymic cleavage of high molecular weight RNA precursors of ASBV.
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Cites
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme
Guerrier-Takada, C., Gardiner, K., Marsh, T., Pace, N., & Altman, S. (1983) · Cell · doi:10.1016/0092-8674(83)90117-4 · Cited by 2,684
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Diener, T. O. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.23.9370 · Cited by 201
Ribozyme Structures and Mechanisms
Doherty, E. A., & Doudna, J. A. (2001) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.30.1.457 · Cited by 195
Viroids: an Ariadne's thread into the RNA labyrinth
Daròs, J., Elena, S. F., & Flores, R. (2006) · The EMBO Reports · doi:10.1038/sj.embor.7400706 · Cited by 106
Metadata
- Authors
- Hutchins, C. J., Rathjen, P. D., Forster, A. C., & Symons, R. H.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1986
- Volume
- 14
- Issue
- 9
- Pages
- 3627-3640
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1992302499
- Cited by
- 585
- References
- 1
- FWCI
- 32.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hutchins, C. J., Rathjen, P. D., Forster, A. C., & Symons, R. H. (1986). Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid. Nucleic Acids Research. https://doi.org/10.1093/nar/14.9.3627
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