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Cited by 687

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Molecular cloning of three distinct forms of the Na+,K+-ATPase .alpha.-subunit from rat brain

Shull, Gary E., Greeb, Jeannette, Lingrel, Jerry B.·1986·Biochemistry

Abstract

Rat brain and kidney cDNA libraries were constructed and screened with a cDNA insert corresponding to the mRNA for the sheep kidney Na+,K+-ATPase catalytic subunit. The alpha-subunit cDNAs isolated from the kidney library were derived from a single class of messenger RNA, and the brain cDNAs were derived from three classes of messenger RNA. The most abundant brain cDNA, which spans 5.1 kilobases, encodes the alpha(+) form of the enzyme. The second most abundant brain cDNA, which spans 3.65 kilobases, is identical with that of the kidney form and therefore encodes the alpha isoform. The third class of cDNA, which spans 3.55 kilobases, was present at low abundance and encodes an isoform of the alpha-subunit, designated alpha III, which has not been identified previously. The complete nucleotide sequence and deduced amino acid sequence for each of the brain and kidney cDNAs have been determined. In addition, we have identified a lysine-rich sequence that may function as a movable, ion-selective gate during cation binding and occlusion and have also identified several amino acid sequence variations that appear to explain some of the well-known species and tissue differences in cardiac glycoside sensitivity.

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Metadata

Authors
Shull, G. E., Greeb, J., & Lingrel, J. B.
Venue
Biochemistry
Published
1986-12-16
Volume
25
Issue
25
Pages
8125-8132
ISSN
  • 0006-2960 (ISSN-L)
  • 1520-4995
OpenAlex
W1984829055
Cited by
687
References
26
FWCI
16.85
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Molecular cloning of three distinct forms of the Na+,K+-ATPase .alpha.-subunit from rat brain. https://app.cochat.ai/papers/10.1021/bi00373a001
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Shull, G. E., Greeb, J., & Lingrel, J. B. (1986). Molecular cloning of three distinct forms of the Na+,K+-ATPase .alpha.-subunit from rat brain. Biochemistry. https://doi.org/10.1021/bi00373a001

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