The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis
Abstract
We describe the use of polyacrylamide gel electrophoresis to estimate chain lengths of double- and single-stranded DNA molecules in the size range 20-1000 base pairs (or nucleotides). Double-stranded DNA molecules of known length produced either by organic synthesis or by restriction endonuclease digestion of viral DNAs were used as standards. The relative electrophoretic mobilities of these standards were examined on both nondenaturing (aqueous) polyacrylamide gels and on denaturing gels containing 7 M urea or 98% formamide. Electrophoretic mobility of DNA is a linear function of the log of molecular weight if appropriate conditions are used, although exceptions are noted. Chain lengths can be conveniently estimated by using as standards bacteriophage gamma DNA restriction fragments or commercially available tracking dyes.
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Citations
Cites
Nucleotide sequence of the rightward operator of phage lambda.
Maniatis, T., Jeffrey, A., & Kleid, D. G. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.3.1184 · Cited by 2,030
Self-citationSharp, P. A., Sugden, B., & Sambrook, J. (1973) · Biochemistry · doi:10.1021/bi00740a018 · Cited by 1,551
Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.
Richardson, C. C. (1965) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.54.1.158 · Cited by 607
Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.
Pribnow, D. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.3.784 · Cited by 606
The Nucleotide Sequence of the lac Operator
Gilbert, W., & Maxam, A. (1973) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.70.12.3581 · Cited by 485
CLARK, R. J., & FELSENFELD, G. (1971) · Nature New Biology · doi:10.1038/newbio229101a0 · Cited by 459
Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae
Danna, K., & Nathans, D. (1971) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.68.12.2913 · Cited by 397
Full length and discrete partial reverse transcripts of globin and chorion mRNAs
Efstratiadis, A., Maniatis, T., Kafatos, F. C., Jeffrey, A., & Vournakis, J. N. (1975) · Cell · doi:10.1016/0092-8674(75)90157-9 · Cited by 218
Self-citationCIII. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast
Khorana, H. G., Agarwal, K. L., Büchi, H., Caruthers, M. H., Gupta, N. K., Klbppe, K., Kumar, A., Ohtsuka, E., RajBhandary, U. L., van de Sande, J. H., et al. (1972) · Journal of Molecular Biology · doi:10.1016/0022-2836(72)90146-5 · Cited by 200
Weber, H., & Khorana, H. G. (1972) · Journal of Molecular Biology · doi:10.1016/0022-2836(72)90147-7 · Cited by 174
Sequence of a represser-binding site in the DNA of bacteriophage λ
Maniatis, T., Ptashne, M., BARRELL, B. G., & Donelson, J. E. (1974) · Nature · doi:10.1038/250394a0 · Cited by 136
Self-citationvan de Sande, J. H., Loewen, P. C., & Khorana, H. G. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)44775-3 · Cited by 77
Fractionation of Native and Denatured Deoxyribonucleic Acid on Agarose Columns
Prunell, A., & Bernardi, G. (1973) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)43947-1 · Cited by 66
Maniatis, T., & Ptashne, M. (1973) · Nature · doi:10.1038/246133a0 · Cited by 61
Self-citationElectrophoretic properties of low molecular weight DNA fragments in agarose-acrylamide gels
Dingman, C. W., Kakefuda, T., & Fisher, M. P. (1972) · Analytical Biochemistry · doi:10.1016/0003-2697(72)90062-0 · Cited by 9
Cited by
[57] Sequencing end-labeled DNA with base-specific chemical cleavages
Maxam, A. M., & Gilbert, W. (1980) · Methods in Enzymology · doi:10.1016/s0076-6879(80)65059-9 · Cited by 14,315
Purification of Mouse Immunoglobulin Heavy‐Chain Messenger RNAs from Total Myeloma Tumor RNA
AUFFRAY, C., & ROUGEON, F. (1980) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1980.tb06030.x · Cited by 2,627
Casadaban, M. J., Chou, J., & Cohen, S. N. (1980) · Journal of Bacteriology · doi:10.1128/jb.143.2.971-980.1980 · Cited by 1,045
A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance
Jorgensen, R. A., Rothstein, S. J., & Reznikoff, W. S. (1979) · Molecular and General Genetics MGG · doi:10.1007/bf00267254 · Cited by 571
Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.
Paterson, B. M., Roberts, B. E., & Kuff, E. L. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.10.4370 · Cited by 536
Metadata
- Authors
- Maniatis, T., Jeffrey, A., & Van deSande, H.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1975-08-01
- Volume
- 14
- Issue
- 17
- Pages
- 3787-3794
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2152193561
- Cited by
- 1,121
- References
- 15
- FWCI
- 39.40
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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