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Blebbistatin, a Myosin II Inhibitor, Is Photoinactivated by Blue Light
Abstract
Blebbistatin is a small molecule inhibitor discovered in a screen for inhibitors of nonmuscle myosin IIA. Blebbistatin inhibits the actin-activated MgATPase activity and in vitro motility of class II myosins. In cells, it has been shown to inhibit contraction of the cytokinetic ring. Blebbistatin has some photochemical properties that may affect its behavior in cells. In particular, we have found that exposure to light at wavelengths below 488 nm rapidly inactivates the inhibitory action of blebbistatin using the in vitro motility of myosin as an assay. In addition, the inhibition of cytokinetic ring contraction can be reversed by exposure of the cells to blue light. This property may be useful in locally reversing the action of blebbistatin treatment in a cell. However, caution should be exercised as free radicals may be produced upon irradiation of blebbistatin that could result in cell damage.
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Citations
Cited by
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Hotulainen, P., & Lappalainen, P. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200511093 · Cited by 934
Teo, B. K. K., Wong, S. T., Lim, C. K., Kung, T. Y. S., Yap, C. H., Ramagopal, Y., Romer, L. H., & Yim, E. K. F. (2013) · ACS Nano · doi:10.1021/nn304966z · Cited by 419
The Role of Myosin II in Glioma Invasion of the Brain
Beadle, C., Assanah, M. C., Monzo, P., Vallee, R., Rosenfeld, S. S., & Canoll, P. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e08-03-0319 · Cited by 310
Myosin IIb regulates actin dynamics during synaptic plasticity and memory formation
Rex, C. S., Gavin, C. F., Rubio, M. D., Kramar, E. A., Chen, L. Y., Jia, Y., Huganir, R. L., Muzyczka, N., Gall, C. M., Miller, C. A., et al. (2010) · Neuron · doi:10.1016/j.neuron.2010.07.016 · Cited by 213
Hammer, J. A., Wang, J. C., Saeed, M., & Pedrosa, A. T. (2019) · Annual Review of Immunology · doi:10.1146/annurev-immunol-042718-041341 · Cited by 96
Metadata
- Authors
- Sakamoto, T., Limouze, J., Combs, C. A., Straight, A. F., & Sellers, J. R.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-12-16
- Volume
- 44
- Issue
- 2
- Pages
- 584-588
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2035373939
- Cited by
- 167
- References
- 0
- FWCI
- 1.19
- Type
- journal article
- Language
- en
- Open access
- closed
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- Sakamoto, T., Limouze, J., Combs, C. A., Straight, A. F., & Sellers, J. R. (2005). Blebbistatin, a Myosin II Inhibitor, Is Photoinactivated by Blue Light. Biochemistry. https://doi.org/10.1021/bi0483357
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
