The DOI matches its Crossref record.
Purification of a Human SRCAP Complex That Remodels Chromatin by Incorporating the Histone Variant H2A.Z into Nucleosomes
Abstract
The Snf-2-related CREB-binding protein activator protein (SRCAP) serves as a coactivator for a number of transcription factors known to interact with CBP. Swr1, the closest Saccharomyces cerevisiae ortholog of SRCAP, is a component of the chromatin remodeling complex SWR-C, which catalyzes exchange of the histone variant H2A.Z into nucleosomes. In this report, we use a combination of conventional chromatography and anti-SRCAP immunoaffinity chromatography to purify a native human SRCAP complex with a polypeptide composition similar to that of SWR-C, and we show for the first time that this SRCAP-containing complex supports ATP-dependent exchange of histone dimers containing H2B and H2A.Z into mononucleosomes reconstituted with recombinant H2A, H2B, H3, and H4. Our findings, together with previous evidence implicating H2A.Z in transcriptional regulation, suggest that SRCAP's coactivator function may depend on its ability to promote incorporation of H2A.Z into chromatin.
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Citations
Cited by
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Clapier, C. R., Iwasa, J., Cairns, B. R., & Peterson, C. L. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.26 · Cited by 1,305
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Ho, L., & Crabtree, G. R. (2010) · Nature · doi:10.1038/nature08911 · Cited by 1,114
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Hargreaves, D. C., & Crabtree, G. R. (2011) · Cell Research · doi:10.1038/cr.2011.32 · Cited by 923
Histones: annotating chromatin
Campos, E. I., & Reinberg, D. (2009) · Annual Review of Genetics · doi:10.1146/annurev.genet.032608.103928 · Cited by 852
Luk, E., Ranjan, A., FitzGerald, P. C., Mizuguchi, G., Huang, Y., Wei, D., & Wu, C. (2010) · Cell · doi:10.1016/j.cell.2010.10.019 · Cited by 469
The roles of histone variants in fine-tuning chromatin organization and function
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Interaction of the Glucocorticoid Receptor with the Chromatin Landscape
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ANP32E is a histone chaperone that removes H2A.Z from chromatin
Obri, A., Ouararhni, K., Papin, C., Diebold, M.-L., Padmanabhan, K., Marek, M., Stoll, I., Roy, L., Reilly, P. T., Mak, T. W., et al. (2014) · Nature · doi:10.1038/nature12922 · Cited by 272
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Gévry, N., Chan, H. M., Laflamme, L., Livingston, D. M., & Gaudreau, L. (2007) · Genes & Development · doi:10.1101/gad.1545707 · Cited by 266
Probabilistic assembly of human protein interaction networks from label-free quantitative proteomics
Sardiu, M. E., Cai, Y., Jin, J., Swanson, S. K., Conaway, R. C., Conaway, J. W., Florens, L., & Washburn, M. P. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0706983105 · Cited by 226
Self-citationMossink, B., Negwer, M., Schubert, D., & Nadif Kasri, N. (2021) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-020-03714-5 · Cited by 114
H2A.Z: a molecular rheostat for transcriptional control
Subramanian, V., Fields, P. A., & Boyer, L. A. (2015) · F1000Prime Reports · doi:10.12703/p7-01 · Cited by 102
Hainer, S. J., & Fazzio, T. G. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.08.071 · Cited by 33
Metadata
- Authors
- Ruhl, D. D., Jin, J., Cai, Y., Swanson, S., Florens, L., Washburn, M. P., Conaway, R. C., Conaway, J. W., & Chrivia, J. C.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-04-07
- Volume
- 45
- Issue
- 17
- Pages
- 5671-5677
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2088641994
- Cited by
- 266
- References
- 0
- FWCI
- 5.28
- Type
- journal article
- Language
- en
- Open access
- closed
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