The DOI matches its Crossref record.
Disulfide Cross-Linked Hyaluronan Hydrogels
Abstract
A new disulfide cross-linking strategy was developed to prepare hyaluronic acid (HA) hydrogel from thiol-modified HA. First, dithiobis(propanoic dihydrazide) (DTP) and dithiobis(butyric dihydrazide) (DTB) were synthesized and then coupled to HA with carbodiimide chemistry. Next, disulfide bonds of the initially formed gel were reduced using dithiothreitol (DTT) to give, after exhaustive dialysis, the corresponding thiol-modified macromolecular derivatives HA-DTPH and HA-DTBH. The degree of substitution of HA-DTPH and HA-DTBH could be controlled from 20% to 70% of available glucuronate carboxylic acid groups. The pK(a) values of the HA-thiol derivatives were determined spectrophotometrically to be pK(a) = 8.87 (HA-DTPH) and pK(a) = 9.01 (HA-DTBH). The thiol groups could be oxidized in air to reform disulfide linkages, which resulted in HA-DTPH and HA-DTBH hydrogel films. Further oxidation of these hydrogels with dilute H(2)O(2) created additional cross-links and afforded poorly swellable films. The disulfide cross-linking was reversible, and films could be again reduced to sols with DTT. Release of blue dextran from cross-linked films was used as a model for drug release. The rapid gelation of the HA-DTPH solution under physiological conditions was also achieved, which demonstrated the capacity for in situ cell encapsulation. Thus, L-929 murine fibroblasts were encapsulated in HA-DTPH hydrogel; these cells remained viable and proliferated during 3 days of culture in vitro.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Cell Encapsulation in Biodegradable Hydrogels for Tissue Engineering Applications
Nicodemus, G. D., & Bryant, S. J. (2008) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2007.0332 · Cited by 1,239
Soft Materials by Design: Unconventional Polymer Networks Give Extreme Properties
Zhao, X., Chen, X., Yuk, H., Lin, S., Liu, X., & Parada, G. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01088 · Cited by 1,161
Makris, E. A., Hadidi, P., & Athanasiou, K. A. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.06.037 · Cited by 1,116
Hydrogels in a historical perspective: From simple networks to smart materials
Buwalda, S. J., Boere, K. W. M., Dijkstra, P. J., Feijen, J., Vermonden, T., & Hennink, W. E. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.03.052 · Cited by 994
Schanté, C. E., Zuber, G., Herlin, C., & Vandamme, T. F. (2011) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2011.03.019 · Cited by 708
Tissue Engineering for Cutaneous Wounds
Clark, R. A. F., Ghosh, K., & Tonnesen, M. G. (2007) · Journal of Investigative Dermatology · doi:10.1038/sj.jid.5700715 · Cited by 538
Crosslinked ionic polysaccharides for stimuli-sensitive drug delivery
Alvarez-Lorenzo, C., Blanco-Fernandez, B., Puga, A. M., & Concheiro, A. (2013) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2013.04.016 · Cited by 532
Polymeric protein delivery systems
Lee, K. Y., & Yuk, S. H. (2007) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2007.04.001 · Cited by 425
Cai, S., Liu, Y., Zheng Shu, X., & Prestwich, G. D. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.03.012 · Cited by 422
Self-citationElectrospun three-dimensional hyaluronic acid nanofibrous scaffolds
Ji, Y., Ghosh, K., Shu, X. Z., Li, B., Sokolov, J. C., Prestwich, G. D., Clark, R. A. F., & Rafailovich, M. H. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.02.037 · Cited by 395
Self-citationAn overview of injectable polymeric hydrogels for tissue engineering
Sivashanmugam, A., Arun Kumar, R., Vishnu Priya, M., Nair, S. V., & Jayakumar, R. (2015) · European Polymer Journal · doi:10.1016/j.eurpolymj.2015.05.014 · Cited by 366
Hyaluronic acid hydrogels with controlled degradation properties for oriented bone regeneration
Patterson, J., Siew, R., Herring, S. W., Lin, A. S. P., Guldberg, R., & Stayton, P. S. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.05.047 · Cited by 346
Lee, F., Chung, J. E., & Kurisawa, M. (2008) · Soft Matter · doi:10.1039/b719557e · Cited by 300
Incorporation of heparin into biomaterials
Sakiyama-Elbert, S. E. (2014) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.08.045 · Cited by 222
Chung, C., Lampe, K. J., & Heilshorn, S. C. (2012) · Biomacromolecules · doi:10.1021/bm3015279 · Cited by 132
A mini review on the functional biomaterials based on poly (lactic acid) stereocomplex
Jing, Y., Quan, C., Liu, B., Jiang, Q., & Zhang, C. (2016) · Polymer Reviews · doi:10.1080/15583724.2015.1111380 · Cited by 92
Torres-de la Roche, L. A., Bérard, V., de Wilde, M. S., Devassy, R., Wallwiener, M., & De Wilde, R. L. (2022) · Journal of Clinical Medicine · doi:10.3390/jcm11040931 · Cited by 12
Metadata
- Authors
- Shu, X. Z., Liu, Y., Luo, Y., Roberts, M. C., & Prestwich, G. D.
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-09-27
- Volume
- 3
- Issue
- 6
- Pages
- 1304-1311
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2041778522
- Cited by
- 616
- References
- 0
- FWCI
- 2.63
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
