The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Synthesis of Uniform Protein−Polymer Conjugates
Abstract
We have developed a novel technique to synthesize near-uniform protein-polymer conjugates by initiating atom transfer radical polymerization of monomethoxy poly(ethylene glycol)-methacrylate from 2-bromoisobutyramide derivatives of chymotrypsin (a protein-initiator). Polymerization initiated from the monosubstituted protein-initiator resulted in the conjugate containing a single, near-monodisperse polymer chain per protein molecule with polydispersity index 1.05. Increasing the number of conjugated 2-bromoisobutyramide initiators per molecule of protein increased the molecular weights and polydispersity indices of the final protein-polymer conjugates. The generic nature of this technique was demonstrated by initiating polymerization of nonionic, cationic, and anionic monomers from the protein-initiator. Protein-polymer conjugates synthesized by this novel technique retained 50-86% of the original enzyme activity. The technique described herein should be useful in synthesizing well-defined protein-polymer conjugates exhibiting a wide range of physical and chemical properties.
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Cites
Atom Transfer Radical Polymerization
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Self-citationEffect of pegylation on pharmaceuticals
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Design and Chemical Synthesis of a Homogeneous Polymer-Modified Erythropoiesis Protein
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Streptavidin as a Macroinitiator for Polymerization: In Situ Protein−Polymer Conjugate Formation
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Site-specific PEGylation of a lysine-deficient TNF-α with full bioactivity
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Cited by
Controlled/living radical polymerization: Features, developments, and perspectives
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Self-citationAtom Transfer Radical Polymerization (ATRP): Current Status and Future Perspectives
Matyjaszewski, K. (2012) · Macromolecules · doi:10.1021/ma3001719 · Cited by 2,606
Self-citationOuchi, M., Terashima, T., & Sawamoto, M. (2009) · Chemical Reviews · doi:10.1021/cr900234b · Cited by 1,257
Lutz, J. (2008) · Journal of Polymer Science Part A: Polymer Chemistry · doi:10.1002/pola.22706 · Cited by 1,245
Macromolecular Engineering by Atom Transfer Radical Polymerization
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Metadata
- Authors
- Lele, B. S., Murata, H., Matyjaszewski, K., & Russell, A. J.
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-10-04
- Volume
- 6
- Issue
- 6
- Pages
- 3380-3387
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2004434237
- Cited by
- 318
- References
- 12
- FWCI
- 7.74
- Type
- journal article
- Language
- en
- Open access
- closed
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