In vitro assessment of alkylglyceryl-functionalized chitosan nanoparticles as permeating vectors for the blood-brain barrier.
Abstract
A series of O-substituted alkylglyceryl chitosans with systematically varied alkyl chain length and degree of grafting has been employed for the formulation of aqueous nanoparticulate systems, which were in turn investigated for their effects on a modeled blood-brain-barrier system of mouse-brain endothelial cells. Barrier function measurements employing electric cell-substrate impedance sensing and analyses of tight junction-specific protein profiles have indicated that the alkylglyceryl-modified chitosan nanoparticles impact upon the integrity of the model blood-brain barrier, whereas confocal microscopy experiments have demonstrated the efficient cellular uptake and the perinuclear localization of these nanoparticles. The application of nanoparticles to the model blood-brain barrier effected an increase in its permeability, as demonstrated by following the transport of the tracer molecule fluorescein isothiocyanate.
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Physiochemical properties of drug-loaded nanocarriers such as hydrophilicity, surface charge, and targeting ligands determine adsorption on brain capillary endothelial cells.
“The physiochemical properties of drug loaded nanocarrier like hydrophilicity, surface charge and targeting ligands of nano carrier is the deciding factor for the adsorption on the brain capillary endothelial cells”
From Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs (2020) · cites this paper as Lien et al., 2012
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- Authors
- Lien, C.-F., Molnár, É., Toman, P., Tsibouklis, J., Pilkington, G. J., Górecki, D. C., & Barbu, E.
- Affiliations
- University of Portsmouth (GB)
- School of Pharmacy and Biomedical Sciences, University of Portsmouth, St. Michael’s Building, White Swan Road, PO1 2DT, United Kingdom
- Corresponding author
- Barbu, Eugen
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-03-29
- Volume
- 13
- Issue
- 4
- Pages
- 1067-1073
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2083668330
- Cited by
- 42
- References
- 35
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 5 · 2023: 1 · 2022: 5 · 2021: 4 · 2020: 6 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 9 · 2012: 2
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomacromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Brain Tumour Research
- Biotechnology and Biological Sciences Research Council (BB/F011865/1)
- Interreg
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- chitosan nanoparticles, blood-brain barrier, blood-brain barrier permeability, endothelial cells, tight junctions, electric cell-substrate impedance sensing, nanoparticle uptake, alkyl chain length, grafting degree, brain drug delivery
- MeSH terms
- Animals, Blood-Brain Barrier, Brain, Cell Survival, Cells, Cultured, Particle Size, Permeability, Surface Properties, Drug Delivery Systems, Fluorescein-5-isothiocyanate, Models, Animal, Endothelial Cells, Chitosan, Mice, Nanoparticles
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