Cited by 238Advanced Drug Delivery SystemsNanoparticle-Based Drug DeliveryBarrier Structure and Function Studies

Characterization and evaluation of chitosan nanoparticles for dopamine brain delivery

Trapani, Adriana, De Giglio, Elvira, Cafagna, Damiana, Denora, Nunzio, Agrimi, Gennaro, Cassano, Tommaso, Gaetani, Silvana, Cuomo, Vincenzo +1 more·2011·International Journal of Pharmaceutics

Abstract

The aim of this study was to characterize nanoparticles (NPs) composed of chitosan (CS) and evaluate their potential for brain delivery of the neurotransmitter Dopamine (DA). For this purpose, CS based NPs were incubated with DA at two different concentrations giving rise to nanocarriers denoted as DA/CSNPs (1) and DA/CSNPs (5), respectively. X-ray Photoelectron Spectroscopy (XPS) analysis confirmed that DA was adsorbed onto the external surface of such NPs. The cytotoxic effect of the CSNPs and DA/CSNPs was assessed using the MTT test and it was found that the nanovectors are less cytotoxic than the neurotransmitter DA after 3 h of incubation time. Transport studies across MDCKII-MDR1 cell line showed that DA/CSNPs (5) give rise to a significant transport enhancing effect compared with the control and greater than the corresponding DA/CSNPs (1). Measurement of reactive oxygen species (ROS) suggested a low DA/CSNPs neurotoxicity after 3 h. In vivo brain microdialysis experiments in rat showed that intraperitoneal acute administration of DA/CSNPs (5) (6-12 mg/kg) induced a dose-dependent increase in striatal DA output. Thus, these CS nanoparticles represent an interesting technological platform for DA brain delivery and, hence, may be useful for Parkinson's disease treatment.

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Crossref · 10.1016/j.ijpharm.2011.07.036

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Authors
Trapani, A., De Giglio, E., Cafagna, D., Denora, N., Agrimi, G., Cassano, T., Gaetani, S., Cuomo, V., & Trapani, G.
Affiliations
  • University of Bari Aldo Moro (IT)
  • University of Foggia (IT)
  • Sapienza University of Rome (IT)
Corresponding author
Trapani, Giuseppe
Venue
International Journal of Pharmaceutics
Publisher
Elsevier BV
Published
2011-10
Volume
419
Issue
1-2
Pages
296-307
ISSN
0378-5173 (ISSN-L)
OpenAlex
W2022832566
Cited by
238
References
38
Citations by year
2026: 14 · 2025: 15 · 2024: 26 · 2023: 11 · 2022: 22 · 2021: 17 · 2020: 21 · 2019: 14 · 2018: 10 · 2017: 22 · 2016: 13 · 2015: 18 · 2014: 10 · 2013: 15 · 2012: 9
FWCI
6.87
Type
journal article
Language
en
Open access
closed
Locations
  • International Journal of Pharmaceutics (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Research Information System University of Ferrara (University of Ferrara) (submittedVersion)
  • IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
  • CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion)
Funders
  • Regione Puglia
  • Università degli Studi di Cagliari
  • Ministero dell’Istruzione, dell’Università e della Ricerca
Topics
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Keywords
chitosan nanoparticles, dopamine, brain drug delivery, nanocarriers, cytotoxicity, reactive oxygen species, neurotoxicity, striatal dopamine, Parkinson's disease, intraperitoneal administration
MeSH terms
Animals, Biological Transport, Brain, Cell Line, Dogs, Dopamine, Dose-Response Relationship, Drug, Drug Carriers, Male, Parkinson Disease, Tissue Distribution, Dopamine Agents, Drug Delivery Systems, Rats, Wistar, Reactive Oxygen Species, Microdialysis, Chitosan, Rats, Nanoparticles, Photoelectron Spectroscopy

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