Characterization and evaluation of chitosan nanoparticles for dopamine brain delivery
Abstract
The aim of this study was to characterize nanoparticles (NPs) composed of chitosan (CS) and evaluate their potential for brain delivery of the neurotransmitter Dopamine (DA). For this purpose, CS based NPs were incubated with DA at two different concentrations giving rise to nanocarriers denoted as DA/CSNPs (1) and DA/CSNPs (5), respectively. X-ray Photoelectron Spectroscopy (XPS) analysis confirmed that DA was adsorbed onto the external surface of such NPs. The cytotoxic effect of the CSNPs and DA/CSNPs was assessed using the MTT test and it was found that the nanovectors are less cytotoxic than the neurotransmitter DA after 3 h of incubation time. Transport studies across MDCKII-MDR1 cell line showed that DA/CSNPs (5) give rise to a significant transport enhancing effect compared with the control and greater than the corresponding DA/CSNPs (1). Measurement of reactive oxygen species (ROS) suggested a low DA/CSNPs neurotoxicity after 3 h. In vivo brain microdialysis experiments in rat showed that intraperitoneal acute administration of DA/CSNPs (5) (6-12 mg/kg) induced a dose-dependent increase in striatal DA output. Thus, these CS nanoparticles represent an interesting technological platform for DA brain delivery and, hence, may be useful for Parkinson's disease treatment.
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A dose-dependent increase in dopamine level was observed after administration of DA-loaded chitosan NPs.
“A dose-dependent increase in dopamine level was observed after the administration of these NPs.116”
From Targeted therapy in chronic diseases using nanomaterial-based drug delivery vehicles (2019) · cites this paper as [116]
DA-loaded chitosan NPs facilitate transport across the blood-brain barrier and minimize cytotoxicity.
“DA-loaded chitosan NPs facilitate transport across the BBB and minimize the cytotoxicity.”
From Targeted therapy in chronic diseases using nanomaterial-based drug delivery vehicles (2019) · cites this paper as [116]
Cites
The rat brain in stereotaxic coordinates
Palkovits, M. (1983) · Neuropeptides · doi:10.1016/0143-4179(83)90049-5 · Cited by 28,959 · see citing works
The Rat Brain in Stereotaxic Coordinates
Paxinos, G., & Watson, C. (1983) · Elsevier eBooks · doi:10.1016/c2009-0-63235-9 · Cited by 28,585 · see citing works
Hansen, M., Nielsen, S. E., & Berg, K. (1989) · Journal of Immunological Methods · doi:10.1016/0022-1759(89)90397-9 · Cited by 3,526 · see citing works
Therapeutic Nanoparticles for Drug Delivery in Cancer
Cho, K., Wang, X., Nie, S., Chen, Z. (., & Shin, D. M. (2008) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-07-1441 · Cited by 2,919 · see citing works
Chitosan-based formulations for delivery of DNA and siRNA
Mao, S., Sun, W., & Kissel, T. (2009) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2009.08.004 · Cited by 970 · see citing works
Polysaccharide colloidal particles as delivery systems for macromolecules
Janes, K. A., Calvo, P., & Alonso, M. J. (2001) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(00)00123-x · Cited by 900 · see citing works
Chitosan nanoparticles as delivery systems for doxorubicin
Janes, K. A., Fresneau, M. P., Marazuela, A., Fabra, À., & Alonso, M. J. (2001) · Journal of Controlled Release · doi:10.1016/s0168-3659(01)00294-2 · Cited by 721 · see citing works
CNS Delivery Via Adsorptive Transcytosis
Hervé, F., Ghinea, N., & Scherrmann, J.-M. (2008) · The AAPS Journal · doi:10.1208/s12248-008-9055-2 · Cited by 676 · see citing works
Microencapsulated chitosan nanoparticles for lung protein delivery
Grenha, A., Seijo, B., & Remuñán‐López, C. (2005) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2005.04.009 · Cited by 469 · see citing works
Ocular drug delivery by liposome–chitosan nanoparticle complexes (LCS-NP)
Diebold, Y., Jarrı́n, M., Sáez, V., Carvalho, E. L. S., Orea, M. J., Calonge, M., Seijo, B., & Alonso, M. J. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.11.028 · Cited by 273 · see citing works
Park, J. S., Han, T. H., Lee, K. Y., et al. (2006) · Journal of Controlled Release · doi:10.1016/j.jconrel.2006.07.011 · Cited by 251 · see citing works
Hanrott, K., Gudmunsen, L., O’Neill, M. J., & Wonnacott, S. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m511560200 · Cited by 231 · see citing works
Colloidal stability of Pluronic F68-coated PLGA nanoparticles: A variety of stabilisation mechanisms
Santander-Ortega, M. J., Jódar‐Reyes, A. B., Csaba, N., Bastos‐González, D., & Ortega-Vinuesa, J. L. (2006) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2006.07.031 · Cited by 222 · see citing works
Gaillard, P. J., & de Boer, A. G. (2000) · European Journal of Pharmaceutical Sciences · doi:10.1016/s0928-0987(00)00152-4 · Cited by 175 · see citing works
In vivo, in vitro and in silico methods for small molecule transfer across the BBB
Mensch, J., Oyarzábal, J., Mackie, C. E., & Augustijns, P. (2009) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.21745 · Cited by 160 · see citing works
Trapani, A., Lopedota, A. A., Franco, M., et al. (2010) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2010.01.010 · Cited by 153 · see citing works
Self-citationA new drug nanocarrier consisting of chitosan and hydoxypropylcyclodextrin
Maestrelli, F., García-Fuentes, M., Mura, P., et al. (2006) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2005.12.006 · Cited by 132 · see citing works
Hollow Chitosan/Poly(acrylic acid) Nanospheres as Drug Carriers
Hu, Y., Ding, Y., Ding, D., Sun, M., Zhang, L., Jiang, X., & Yang, C. (2007) · Biomacromolecules · doi:10.1021/bm0608176 · Cited by 131 · see citing works
The performance of nanocarriers for transmucosal drug delivery
Csaba, N., García-Fuentes, M., & Alonso, M. J. (2006) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.3.4.463 · Cited by 126 · see citing works
Jellinger, K. A., Linert, L., Kienzl, E., Herlinger, E., & Youdim, M. B. H. (1995) · PubMed · Cited by 121 · see citing works
Enochs, W. S., Sarna, T., Zecca, L., Riley, P. A., & Swartz, H. M. (1994) · Journal of Neural Transmission - Parkinsons Disease and Dementia Section · doi:10.1007/bf02260963 · Cited by 116 · see citing works
Novel drug nanocarriers combining hydrophilic cyclodextrins and chitosan
Trapani, A., García-Fuentes, M., & Alonso, M. J. (2008) · Nanotechnology · doi:10.1088/0957-4484/19/18/185101 · Cited by 86 · see citing works
Self-citationPillay, S., Pillay, V., Choonara, Y. E., Naidoo, D., Khan, R. A., du Toit, L. C., Ndesendo, V. M. K., Modi, G., Danckwerts, M. P., & Iyuke, S. E. (2009) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2009.08.021 · Cited by 80 · see citing works
Lopedota, A. A., Trapani, A., Cutrignelli, A., Chiarantini, L., Pantucci, E., Curci, R., Manuali, E., & Trapani, G. (2009) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2009.02.013 · Cited by 80 · see citing works
Self-citationA facile method for preparing highly conductive and reflective surface-silvered polyimide films
Liao, Y., Cao, B., Wang, W., et al. (2009) · Applied Surface Science · doi:10.1016/j.apsusc.2009.05.038 · Cited by 78 · see citing works
Formulation andin vitro-in vivoevaluation of piribedil solid lipid micro- and nanoparticles
Demirel, Y. Y. M. (2001) · Journal of Microencapsulation · doi:10.1080/02652040010018119 · Cited by 71 · see citing works
Pinnen, F., Cacciatore, I., Cornacchia, C., Sozio, P., Iannitelli, A., Costa, M., Pecci, L., Nasuti, C., Cantalamessa, F., & Stefano, A. D. (2007) · Journal of Medicinal Chemistry · doi:10.1021/jm070037v · Cited by 67 · see citing works
Synthesis and properties of chitosan-N-dextran graft copolymers
Janciauskaite, U., Rakutyte, V., Miškinis, J., et al. (2008) · Reactive and Functional Polymers · doi:10.1016/j.reactfunctpolym.2007.12.001 · Cited by 60 · see citing works
Liao, J., Lin, S., & Wu, Y.-T. (2004) · Biomacromolecules · doi:10.1021/bm0494951 · Cited by 60 · see citing works
Analytical characterization of chitosan nanoparticles for peptide drug delivery applications
Ieva, E., Trapani, A., Cioffi, N., et al. (2008) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-008-2463-4 · Cited by 58 · see citing works
Self-citationChitosan citrate as multifunctional polymer for vaginal delivery
Bonferoni, M. C., Sandri, G., Rossi, S., et al. (2007) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2007.11.004 · Cited by 57 · see citing works
Supramolecular devices to improve the treatment of brain diseases
Paolino, D., Cosco, D., Molinaro, R., Celia, C., & Fresta, M. (2011) · Drug Discovery Today · doi:10.1016/j.drudis.2011.02.006 · Cited by 53 · see citing works
Novel L-Dopa and Dopamine Prodrugs Containing a 2-Phenyl-imidazopyridine Moiety
Denora, N., Laquintana, V., Lopedota, A. A., et al. (2007) · Pharmaceutical Research · doi:10.1007/s11095-007-9255-y · Cited by 46 · see citing works
Self-citationMonoaminergic Changes in Locus Coeruleus and Dorsal Raphe Nucleus Following Noradrenaline Depletion
Cassano, T., Gaetani, S., Morgese, M. G., et al. (2009) · Neurochemical Research · doi:10.1007/s11064-009-9928-5 · Cited by 43 · see citing works
Self-citationPuttonen, K. A., Lehtonen, Š., Raasmaja, A., et al. (2006) · Toxicology in Vitro · doi:10.1016/j.tiv.2006.07.001 · Cited by 33 · see citing works
Pharmacoscintigraphic Evaluation of Polysorbate80-Coated Chitosan Nanoparticles for Brain Targeting
Soni, S., Babbar, A. K., Sharma, R. K., et al. (2005) · American Journal of Drug Delivery · doi:10.2165/00137696-200503030-00004 · Cited by 20 · see citing works
Advances in the treatment of Parkinson's disease
Pahwa, R., & Koller, W. C. (1998) · Drugs of today · doi:10.1358/dot.1998.34.2.485183 · Cited by 15 · see citing works
Advances in the treatment of Parkinson disease.
信夫, 柳. (1993) · Nihon Naika Gakkai Zasshi · doi:10.2169/naika.82.1109 · Cited by 1 · see citing works
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Targeted therapy in chronic diseases using nanomaterial-based drug delivery vehicles
Singh, A. P., Biswas, A., Shukla, A., & Maiti, P. (2019) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-019-0068-3 · Cited by 645 · see citing works
Drug delivery to the central nervous system by polymeric nanoparticles: What do we know?
Kreuter, J. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2013.08.008 · Cited by 554 · see citing works
Recent Advancements of Nanomedicine in Neurodegenerative Disorders Theranostics
Mukherjee, S., Madamsetty, V. S., Bhattacharya, D., Roy Chowdhury, S., Paul, M. K., & Mukherjee, A. (2020) · Advanced Functional Materials · doi:10.1002/adfm.202003054 · Cited by 152 · see citing works
Leyva-Gómez, G., Cortés, H., Magaña, J. J., Leyva-García, N., Quintanar-Guerrero, D., & Florán, B. (2015) · Drug Discovery Today · doi:10.1016/j.drudis.2015.02.009 · Cited by 102 · see citing works
Lien, C.-F., Molnár, É., Toman, P., Tsibouklis, J., Pilkington, G. J., Górecki, D. C., & Barbu, E. (2012) · Biomacromolecules · doi:10.1021/bm201790s · Cited by 42 · see citing works
- Authors
- Trapani, A., De Giglio, E., Cafagna, D., Denora, N., Agrimi, G., Cassano, T., Gaetani, S., Cuomo, V., & Trapani, G.
- Affiliations
- University of Bari Aldo Moro (IT)
- University of Foggia (IT)
- Sapienza University of Rome (IT)
- Corresponding author
- Trapani, Giuseppe
- Venue
- International Journal of Pharmaceutics
- Publisher
- Elsevier BV
- Published
- 2011-10
- Volume
- 419
- Issue
- 1-2
- Pages
- 296-307
- ISSN
- 0378-5173 (ISSN-L)
- OpenAlex
- W2022832566
- Cited by
- 238
- References
- 38
- Citations by year
- 2026: 14 · 2025: 15 · 2024: 26 · 2023: 11 · 2022: 22 · 2021: 17 · 2020: 21 · 2019: 14 · 2018: 10 · 2017: 22 · 2016: 13 · 2015: 18 · 2014: 10 · 2013: 15 · 2012: 9
- FWCI
- 6.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- International Journal of Pharmaceutics (publishedVersion)
- PubMed (publishedVersion)
- Institutional Research Information System University of Ferrara (University of Ferrara) (submittedVersion)
- IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
- CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion)
- Funders
- Regione Puglia
- Università degli Studi di Cagliari
- Ministero dell’Istruzione, dell’Università e della Ricerca
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- chitosan nanoparticles, dopamine, brain drug delivery, nanocarriers, cytotoxicity, reactive oxygen species, neurotoxicity, striatal dopamine, Parkinson's disease, intraperitoneal administration
- MeSH terms
- Animals, Biological Transport, Brain, Cell Line, Dogs, Dopamine, Dose-Response Relationship, Drug, Drug Carriers, Male, Parkinson Disease, Tissue Distribution, Dopamine Agents, Drug Delivery Systems, Rats, Wistar, Reactive Oxygen Species, Microdialysis, Chitosan, Rats, Nanoparticles, Photoelectron Spectroscopy
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