Stiffness Alterations of Single Cells Induced by UV in the Presence of NanoTiO2
Abstract
Nanocrystalline titanium dioxide (nanoTiO2) has been reported to generate reactive oxygen species (ROS) under UV illumination. In our studies, changes in mechanical properties of human skin fibroblasts, exposed to the oxidative stress induced in the presence of nanoTiO2 and UV light, were studied using atomic force microscopy (AFM). The exposure of cells to the action of ROS was performed at low TiO2 concentration (4 microg/mL) and under illumination with low-intensity UVA (8 and 20 mW/cm2) or UVC (0.1 mW/ cm2). AFM measurements of the cell stiffness were carried out immediately after exposure of cells to the oxidative stress. The data suggest that under illumination with low-intensity UVA nanoTiO2 generates ROS, which, in turn, damage cellular and subcellular structures. This process was detected by AFM as a marked drop in the cellular stiffness of ca. 30-75%, which occurred rapidly, in the time frame of 1 min. The photo-oxidative stress inducing the decrease of cell stiffness was cancelled in the presence of a well-established antioxidant, beta-carotene. The results highlight the sensitivity of AFM to detect early changes in mechanical properties of cells exposed to oxidative stress.
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AFM measurements showing decreased stiffness of phototreated human skin fibroblasts support implication of hydroxyl radicals in UVA activation of nanocrystalline TiO2.
“Support for the implication of • OH in the UVA activation of nanocrystalline TiO 2 was provided by atomic force microscopy (AFM) measurements showing a marked decrease in the stiffness of phototreated human skin fibroblasts (201).”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [201]
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Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup>
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- Authors
- Vileno, B., Lekka, M., Sienkiewicz, A., Jeney, S., Stoessel, G., Lekki, J., Forró, L., & Stachura, Z.
- Affiliations
- Institute of Physics (PL)
- Institute of Physics of Complex Matter, École Polytechnique Fédérale de Lausanne, CH−1015 Lausanne, Switzerland, The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, PL-31−342 Kraków, Poland, and Institute of Physics, Polish Academy of Sciences, Al. Lotników 32/46, PL-02-668 Warsaw, Poland
- Corresponding author
- Lekka, Małgorzata
- Venue
- Environmental Science & Technology
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-06-09
- Volume
- 41
- Issue
- 14
- Pages
- 5149-5153
- ISSN
- 0013-936X (ISSN-L)
- 1520-5851
- OpenAlex
- W2005545629
- Cited by
- 60
- References
- 17
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 4 · 2022: 2 · 2021: 1 · 2019: 4 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 5 · 2014: 5 · 2013: 2 · 2012: 8
- FWCI
- 2.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Environmental Science & Technology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Keywords
- UV irradiation, cell stiffness, dermal fibroblasts, reactive oxygen species, oxidative stress, UVA, UV-C, beta-carotene, antioxidants, nanoparticle toxicity, cell mechanical properties, atomic force microscopy
- MeSH terms
- Humans, Titanium, Ultraviolet Rays, Reactive Oxygen Species, Microscopy, Atomic Force, Metal Nanoparticles
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