The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Detection and Imaging of Zinc Secretion from Pancreatic β-Cells Using a New Fluorescent Zinc Indicator
Abstract
A novel Zn2+-selective visible wavelength fluoroionophore (FluoZin-3, 9) was synthesized. The chelating portion of the molecule resembles known EGTA-based Ca2+-selective fluoroionophores, except that one of the N-acetic acid moieties has been deleted in 9. FluoZin-3 is virtually non-fluorescent in the absence of Zn2+, and exhibits a several hundred-fold fluorescence increase upon saturation with Zn2+( approximately 100 nM), with a Kd = 15 +/- 2 nM. A 1:1 binding stoichiometry of 9:Zn2+ was determined, and the fluorescence of the complex is pH-independent at pH > 6. FluoZin-3 was used to monitor Zn2+ that was co-secreted with insulin from pancreatic beta-cells by exocytosis following stimulation with glucose. The total Zn2+ concentration near the cells reached 600 nM, and Zn2+ was detectable at least 15 mum away from secreting cells. Heterogeneity in secretion among cells was indicated in that some cells in a cluster did not release Zn2+. Also, within secreting cells some regions of the cell membrane gave rise to secretion while others did not, suggesting active zones of secretion on the cell surface.
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Citations
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Cited by
Glucagon-like peptide 1 (GLP-1)
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The New Biology and Pharmacology of Glucagon
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Metadata
- Authors
- Gee, K. R., Zhou, Z.-L., Qian, W.-J., & Kennedy, R.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-01-12
- Volume
- 124
- Issue
- 5
- Pages
- 776-778
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2051485869
- Cited by
- 406
- References
- 21
- FWCI
- 8.75
- Type
- journal article
- Language
- en
- Open access
- closed
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