DNA-based magnetic nanoparticle assembly acts as a magnetic relaxation nanoswitch allowing screening of DNA-cleaving agents
Abstract
Monodisperse magnetic nanoparticles conjugated with complementary oligonucleotide sequences self-assemble into stable magnetic nanoassemblies resulting in a decrease of the spin-spin relaxation times (T2) of neighboring water protons. When these nanoassemblies are treated with a DNA cleaving agent, the nanoparticles become dispersed, switching the T2 of the solution back to original values. These qualities render the developed nanoparticles and their nanoassemblies as magnetic relaxation switches capable of screening for DNA-cleaving compounds by magnetic resonance methods such as MRI and NMR.
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- Authors
- Perez, J. M., O'Loughin, T., Simeone, F. J., Weissleder, R., & Josephson, L.
- Affiliations
- MGH-Center for Molecular Imaging Research, Harvard Medical School, 149 13th Street, Charlestown, Massachusetts 02129
- Harvard University (US)
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-02-28
- Volume
- 124
- Issue
- 12
- Pages
- 2856-2857
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2010081112
- Cited by
- 355
- References
- 8
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 5 · 2022: 8 · 2021: 5 · 2020: 6 · 2019: 13 · 2018: 12 · 2017: 11 · 2016: 17 · 2015: 13 · 2014: 12 · 2013: 15 · 2012: 27
- FWCI
- 16.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the American Chemical Society (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Magnetism in coordination complexes
- Keywords
- magnetic nanoparticles, oligonucleotide conjugation, magnetic relaxation, T2 relaxation time, T2 relaxation, drug screening, nanoparticle dispersion, MRI contrast agents, NMR probes
- MeSH terms
- DNA, Electrophoresis, Oligonucleotides, Viral Proteins, Deoxyribonuclease BamHI, Site-Specific DNA-Methyltransferase (Adenine-Specific), Biosensing Techniques, Nuclear Magnetic Resonance, Biomolecular, Nanotechnology
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